LOCUS       sequence-33357-e         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Gallo CM, Munro E, Rasoloson D, Merritt C, Seydoux G
  TITLE     Processing bodies and germ granules are distinct RNA granules that 
            interact in C. elegans embryos.
  JOURNAL   Dev Biol. 2008 Nov 1. 323(1):76-87.
  PUBMED    18692039
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2008 Nov 1. 323(1):76-87."
COMMENT     Sequence Label: pCG111
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..77
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             90..134
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    141..165
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(477..501)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactatacaa atgcccgggg gatcggtgga gctccaccgg tggcggccgc tctagagaga
       61 atctttattt tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc
      121 agcacatgga cagcggatca acaagtttgt acaaaaaagc aggcttaatg gccaccgaaa
      181 agaaagaagt gacactgtct gctacagtcg aagagccact cgatttgctc cgtctcagtt
      241 tggatgagag agtttatgtc aaaatgagaa acgacagaga gttgcgcggt cgtctcagag
      301 ctttcgatca acatttgaac atggttttat cggaggttga agagacaatc accacccgtg
      361 aagtcgacga agacacattc gaagagatct acaaacaaac aaaacgtgtt gttccaatgc
      421 ttttcgttcg tggtgattct gtcattctcg tgtctccacc gattcgtgca tcctaaaccc
      481 agctttcttg tacaaagtgg ttgatttgtt ttgtatattt atccactcac cccctctctt
      541 tgtcctgtga atgaacttgt gccaaaaaag ccaaaaataa tttacttttt ttaaagacat
      601 attaaaactt gaaaacttgg aacaatacgg tgcgaaagcg aaacagagac agaaaaatga
      661 acaaagagat ccgtgaaaag tgaacgatga tttaggcttc ttgttgggcg gcgtggatga
      721 gagtgatgtt gtctcc
//
