LOCUS       sequence-333601-        8202 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human immunoglobulin epsilon antibody expression vector (lambda 
            light chain) without variable regions. Enables Colony-PCR screening 
            of false-positive (PCR vector template) colonies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Dodev TS, Karagiannis P, Gilbert AE, Josephs DH, Bowen H, James LK, 
            Bax HJ, Beavil R, Pang MO, Gould HJ, Karagiannis SN, Beavil AJ
  TITLE     A tool kit for rapid cloning and expression of recombinant 
            antibodies.
  JOURNAL   Sci Rep. 2014 Jul 30;4:5885. doi: 10.1038/srep05885.
  PUBMED    25073855
REFERENCE   2  (bases 1 to 8202)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-07-30"; volume: "4:5885. doi"; pages: " 
            10.1038/srep05885"
COMMENT     Sequence Label: pVITRO1-dV-IgE/lambda
FEATURES             Location/Qualifiers
     source          1..8202
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        256..1562
                     /label=mEF-1-alpha promoter
                     /note="strong constitutive promoter for mouse elongation 
                     factor EF-1-alpha"
     intron          583..1530
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     polyA_signal    2943..3064
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2954..2973)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3008..3027
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3252..3840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3741..3760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        3920..4223
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4338..5643
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    4680..5611
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     misc_feature    6049..6493
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        6526..6572
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        6564..6582
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6592..7617
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    7629..8201
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagtgg agccgagagt aattcataca aaaggaggga tcgccttcgc aaggggagag
      301 cccagggacc gtccctaaat tctcacagac ccaaatccct gtagccgccc cacgacagcg
      361 cgaggagcat gcgctcaggg ctgagcgcgg ggagagcaga gcacacaagc tcatagaccc
      421 tggtcgtggg ggggaggacc ggggagctgg cgcggggcaa actgggaaag cggtgtcgtg
      481 tgctggctcc gccctcttcc cgagggtggg ggagaacggt atataagtgc ggcagtcgcc
      541 ttggacgttc tttttcgcaa cgggtttgcc gtcagaacgc aggtgagggg cgggtgtggc
      601 ttccgcgggc cgccgagctg gaggtcctgc tccgagcggg ccgggccccg ctgtcgtcgg
      661 cggggattag ctgcgagcat tcccgcttcg agttgcgggc ggcgcgggag gcagagtgcg
      721 aggcctagcg gcaaccccgt agcctcgcct cgtgtccggc ttgaggccta gcgtggtgtc
      781 cgcgccgccg ccgcgtgcta ctccggccgc actctggtct tttttttttt tgttgttgtt
      841 gccctgctgc cttcgattgc cgttcagcaa taggggctaa caaagggagg gtgcggggct
      901 tgctcgcccg gagcccggag aggtcatggt tggggaggaa tggagggaca ggagtggcgg
      961 ctggggcccg cccgccttcg gagcacatgt ccgacgccac ctggatgggg cgaggcctgg
     1021 ggtttttccc gaagcaacca ggctggggtt agcgtgccga ggccatgtgg ccccagcacc
     1081 cggcacgatc tggcttggcg gcgccgcgtt gccctgcctc cctaactagg gtgaggccat
     1141 cccgtccggc accagttgcg tgcgtggaaa gatggccgct cccgggccct gttgcaagga
     1201 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     1261 aagagggcct ggtccctcac cggctgctgc ttcctgtgac cccgtggtcc tatcggccgc
     1321 aatagtcacc tcgggctttt gagcacggct agtcgcggcg gggggagggg atgtaatggc
     1381 gttggagttt gttcacattt ggtgggtgga gactagtcag gccagcctgg cgctggaagt
     1441 catttttgga atttgtcccc ttgagttttg agcggagcta attctcgggc ttcttagcgg
     1501 ttcaaaggta tcttttaaac ccttttttag gtgttgtgaa aaccaccgct aattcaaagc
     1561 aaatggactg gacctggagg atcctcttct tggtggcggc cgccacaggc gcgcactccg
     1621 aattcgatat cctcgaggct agcacacaga gcccatccgt cttccccttg acccgctgct
     1681 gcaaaaacat tccctccaat gccacctccg tgactctggg ctgcctggcc acgggctact
     1741 tcccggagcc ggtgatggtg acctgggaca caggctccct caacgggaca actatgacct
     1801 taccagccac caccctcacg ctctctggtc actatgccac catcagcttg ctgaccgtct
     1861 cgggtgcgtg ggccaagcag atgttcacct gccgtgtggc acacactcca tcgtccacag
     1921 actgggtcga caacaaaacc ttcagcgtct gctccaggga cttcaccccg cccaccgtga
     1981 agatcttaca gtcgtcctgc gacggcggcg ggcacttccc cccgaccatc cagctcctgt
     2041 gcctcgtctc tgggtacacc ccagggacta tcaacatcac ctggctggag gacgggcagg
     2101 tcatggacgt ggacttgtcc accgcctcta ccacgcagga gggtgagctg gcctccacac
     2161 aaagcgagct caccctcagc cagaagcact ggctgtcaga ccgcacctac acctgccagg
     2221 tcacctatca aggtcacacc tttgaggaca gcaccaagaa gtgtgcagat tccaacccga
     2281 gaggggtgag cgcctaccta agccggccca gcccgttcga cctgttcatc cgcaagtcgc
     2341 ccacgatcac ctgtctggtg gtggacctgg cacccagcaa ggggaccgtg aacctgacct
     2401 ggtcccgggc cagtgggaag cctgtgaacc actccaccag aaaggaggag aagcagcgca
     2461 atggcacgtt aaccgtcacg tccaccctgc cggtgggcac ccgagactgg atcgaggggg
     2521 agacctacca gtgcagggtg acccaccccc acctgcccag ggccctcatg cggtccacga
     2581 ccaagaccag cggcccgcgt gctgccccgg aagtctatgc gtttgcgacg ccggagtggc
     2641 cggggagccg ggacaagcgc accctcgcct gcctgatcca gaacttcatg cctgaggaca
     2701 tctcggtgca gtggctgcac aacgaggtgc agctcccgga cgcccggcac agcacgacgc
     2761 agccccgcaa gaccaagggc tccggcttct tcgtcttcag ccgcctggag gtgaccaggg
     2821 ccgaatggga gcagaaagat gagttcatct gccgtgcagt ccatgaggca gcgagcccct
     2881 cacagaccgt ccagcgagcg gtgtctgtaa atcccggtaa atgattagct ggccagacat
     2941 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     3001 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     3061 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
     3121 tttttaaagc aagtaaaacc tctacaaatg tggtatggaa atgttaatta actagccatg
     3181 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3241 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3301 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3361 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3421 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3481 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3541 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3601 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3661 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3721 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3781 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3841 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3901 ttcttaatta acctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     3961 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     4021 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     4081 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     4141 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     4201 tattagtcat cgctattacc atgatgatgc ggttttggca gtacatcaat gggcgtggat
     4261 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     4321 tttgactagt ggagccgaga gtaattcata caaaaggagg gatcgccttc gcaaggggag
     4381 agcccaggga ccgtccctaa attctcacag acccaaatcc ctgtagccgc cccacgacag
     4441 cgcgaggagc atgcgcccag ggctgagcgc gggtagatca gagcacacaa gctcacagtc
     4501 cccggcggtg gggggagggg cgcgctgagc gggggccagg gagctggcgc ggggcaaact
     4561 gggaaagtgg tgtcgtgtgc tggctccgcc ctcttcccga gggtggggga gaacggtata
     4621 taagtgcggt agtcgccttg gacgttcttt ttcgcaacgg gtttgccgtc agaacgcagg
     4681 tgagtggcgg gtgtggcttc cgcgggcccc ggagctggag ccctgctctg agcgggccgg
     4741 gctgatatgc gagtgtcgtc cgcagggttt agctgtgagc attcccactt cgagtggcgg
     4801 gcggtgcggg ggtgagagtg cgaggcctag cggcaacccc gtagcctcgc ctcgtgtccg
     4861 gcttgaggcc tagcgtggtg tccgccgccg cgtgccactc cggccgcact atgcgttttt
     4921 tgtccttgct gccctcgatt gccttccagc agcatgggct aacaaaggga gggtgtgggg
     4981 ctcactctta aggagcccat gaagcttacg ttggatagga atggaagggc aggaggggcg
     5041 actggggccc gcccgccttc ggagcacatg tccgacgcca cctggatggg gcgaggcctg
     5101 tggctttccg aagcaatcgg gcgtgagttt agcctacctg ggccatgtgg ccctagcact
     5161 gggcacggtc tggcctggcg gtgccgcgtt cccttgcctc ccaacaaggg tgaggccgtc
     5221 ccgcccggca ccagttgctt gcgcggaaag atggccgctc ccggggccct gttgcaagga
     5281 gctcaaaatg gaggacgcgg cagcccggtg gagcgggcgg gtgagtcacc cacacaaagg
     5341 aagagggcct tgcccctcgc cggccgctgc ttcctgtgac cccgtggtct atcggccgca
     5401 tagtcacctc gggcttctct tgagcaccgc tcgtcgcggc ggggggaggg gatctaatgg
     5461 cgttggagtt tgttcacatt tggtgggtgg agactagtca ggccagcctg gcgctggaag
     5521 tcattcttgg aatttgcccc tttgagtttg gagcgaggct aattctcaag cctcttagcg
     5581 gttcaaaggt attttctaaa cccgtttcca ggtgttgtga aagccaccgc taattcaaag
     5641 caatatggcc tggatgatgc ttctcctcgg actccttgct tatggatccg gagtcgactc
     5701 tgaattcgat atcctcgagc taggtcagcc caaggcggcg ccctcggtca ctctgttccc
     5761 gccctcctct gaggagcttc aagccaacaa ggccacactg gtgtgtctca taagtgactt
     5821 ctacccggga gccgtgacag tggcctggaa ggcagatagc agccccgtca aggcgggagt
     5881 ggagaccacc acaccctcca aacaaagcaa caacaagtac gcggccagca gctatctgag
     5941 cctgacgcct gagcagtgga agtcccacag aagctacagc tgccaggtca cgcatgaagg
     6001 gagcaccgtg gagaagacag tggcccctac agaatgttca tagctaggag caggtttccc
     6061 caatgacaca aaacgtgcaa cttgaaactc cgcctggtct ttccaggtct agaggggtaa
     6121 cactttgtac tgcgtttggc tccacgctcg atccactggc gagtgttagt aacagcactg
     6181 ttgcttcgta gcggagcatg acggccgtgg gaactcctcc ttggtaacaa ggacccacgg
     6241 ggccaaaagc cacgcccaca cgggcccgtc atgtgtgcaa ccccagcacg gcgactttac
     6301 tgcgaaaccc actttaaagt gacattgaaa ctggtaccca cacactggtg acaggctaag
     6361 gatgcccttc aggtaccccg aggtaacacg cgacactcgg gatctgagaa ggggactggg
     6421 gcttctataa aagcgctcgg tttaaaaagc ttctatgcct gaataggtga ccggaggtcg
     6481 gcacctttcc tttgcaatta ctgaccctat gaatacactg actgtttgac aattaatcat
     6541 cggcatagta tatcggcata gtataatacg actcactata ggagggccac catgaagaaa
     6601 cctgaactga cagcaacttc tgttgagaag tttctcattg aaaaatttga ttctgtttct
     6661 gatctcatgc agctgtctga aggtgaagaa agcagagcct tttcttttga tgttggagga
     6721 agaggttatg ttctgagggt caattcttgt gctgatggtt tttacaaaga cagatatgtt
     6781 tacagacact ttgcctctgc tgctctgcca attccagaag ttctggacat tggagaattt
     6841 tctgaatctc tcacctactg catcagcaga agagcacaag gagtcactct ccaggatctc
     6901 cctgaaactg agctgccagc tgttctgcaa cctgttgctg aagcaatgga tgccattgca
     6961 gcagctgatc tgagccaaac ctctggattt ggtccttttg gtccccaagg cattggtcag
     7021 tacaccactt ggagggattt catttgtgcc attgctgatc ctcatgtcta tcactggcag
     7081 actgtgatgg atgacacagt ttctgcttct gttgctcagg cactggatga actcatgctg
     7141 tgggcagaag attgtcctga agtcagacac ctggtccatg ctgattttgg aagcaacaat
     7201 gttctgacag acaatggcag aatcactgca gtcattgact ggtctgaagc catgtttgga
     7261 gattctcaat atgaggttgc caacattttt ttttggagac cttggctggc ttgcatggaa
     7321 caacaaacaa gatattttga aagaagacac ccagaactgg ctggttcccc cagactgaga
     7381 gcctacatgc tcagaattgg cctggaccaa ctgtatcaat ctctggttga tggaaacttt
     7441 gatgatgctg cttgggcaca aggaagatgt gatgccattg tgaggtctgg tgctggaact
     7501 gttggaagaa ctcaaattgc aagaaggtct gctgctgttt ggactgatgg atgtgttgaa
     7561 gttctggctg actctggaaa caggagaccc tccacaagac ccagagccaa ggaatgaata
     7621 ttagctagat tatccctaat acctgccacc ccactcttaa tcagtggtgg aagaacggtc
     7681 tcagaactgt ttgtttcaat tggccattta agtttagtag taaaagactg gttaatgata
     7741 acaatgcatc gtaaaacctt cagaaggaaa ggagaatgtt ttgtggacca ctttggtttt
     7801 cttttttgcg tgtggcagtt ttaagttatt agtttttaaa atcagtactt tttaatggaa
     7861 acaacttgac caaaaatttg tcacagaatt ttgagaccca ttaaaaaagt taaatgagaa
     7921 acctgtgtgt tcctttggtc aacaccgaga catttaggtg aaagacatct aattctggtt
     7981 ttacgaatct ggaaacttct tgaaaatgta attcttgagt taacacttct gggtggagaa
     8041 tagggttgtt ttccccccac ataattggaa ggggaaggaa tatcatttaa agctatggga
     8101 gggttgcttt gattacaaca ctggagagaa atgcagcatg ttgctgattg cctgtcacta
     8161 aaacaggcca aaaactgagt ccttgggttg catagaaagc tg
//
