LOCUS       sequence-333605-       11314 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in wheat plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11314)
  AUTHORS   Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li 
            J, Gao C
  TITLE     High-efficiency prime editing with optimized, paired pegRNAs in 
            plants.
  JOURNAL   Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 
            10.1038/s41587-021-00868-w.
  PUBMED    33767395
REFERENCE   2  (bases 1 to 11314)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 
            10.1038/s41587-021-00868-w."
COMMENT     Sequence Label: pSpG-PPE
FEATURES             Location/Qualifiers
     source          1..11314
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2021..2041
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2048..3763
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             6149..6196
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8336..8356
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8414..8590
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9144..9160)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9144..9160)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9157..9179)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9168..9184
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9192..9198,9199..9216,9217..9222))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9237..9258
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9375..9392)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9546..10134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9626..9645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10305..11096,11097..11165))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10928..10947
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11166..11270)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg ccaaagaaga agaggaaggt
     2041 tcttaaggac aagaagtact cgatcggcct cgacatcggg acgaactcag ttggctgggc
     2101 cgtgatcacc gacgagtaca aggtgccctc taagaagttc aaggtcctgg ggaacaccga
     2161 ccgccattcc atcaagaaga acctcatcgg cgctctcctg ttcgacagcg gggagaccgc
     2221 tgaggctacg aggctcaaga gaaccgctag gcgccggtac acgagaagga agaacaggat
     2281 ctgctacctc caagagattt tctccaacga gatggccaag gttgacgatt cattcttcca
     2341 ccgcctggag gagtctttcc tcgtggagga ggataagaag cacgagcggc atcccatctt
     2401 cggcaacatc gtggacgagg ttgcctacca cgagaagtac cctacgatct accatctgcg
     2461 gaagaagctc gtggactcca ccgataaggc ggacctcaga ctgatctacc tcgctctggc
     2521 ccacatgatc aagttccgcg gccatttcct gatcgagggg gatctcaacc cagacaacag
     2581 cgatgttgac aagctgttca tccaactcgt gcagacctac aaccaactct tcgaggagaa
     2641 cccgatcaac gcctctggcg tggacgcgaa ggctatcctg tccgcgaggc tctcgaagtc
     2701 caggaggctg gagaacctga tcgctcagct cccaggcgag aagaagaacg gcctgttcgg
     2761 gaacctcatc gctctcagcc tggggctcac cccgaacttc aagtcgaact tcgatctcgc
     2821 tgaggacgcc aagctgcaac tctccaagga cacctacgac gatgacctcg ataacctcct
     2881 ggcccagatc ggcgatcaat acgcggacct gttcctcgct gccaagaacc tgtcggacgc
     2941 catcctcctg tcagatatcc tccgcgtgaa caccgagatc acgaaggctc cactctctgc
     3001 ctccatgatc aagcgctacg acgagcacca tcaggatctg accctcctga aggcgctggt
     3061 ccgccaacag ctcccggaga agtacaagga gattttcttc gatcagtcga agaacggcta
     3121 cgctgggtac atcgacggcg gggcctcaca agaggagttc tacaagttca tcaagccaat
     3181 cctggagaag atggacggca cggaggagct cctggtgaag ctcaacaggg aggacctcct
     3241 gcggaagcag agaaccttcg ataacggcag catcccccac caaatccatc tcggggagct
     3301 gcacgccatc ctgagaaggc aagaggactt ctaccctttc ctcaaggata accgggagaa
     3361 gatcgagaag atcctgacct tcagaatccc atactacgtc ggccctctcg cgcgggggaa
     3421 ctcaagattc gcttggatga cccgcaagtc tgaggagacc atcacgccgt ggaacttcga
     3481 ggaggtggtg gacaagggcg ctagcgctca gtcgttcatc gagaggatga ccaacttcga
     3541 caagaacctg cccaacgaga aggtgctccc taagcactcg ctcctgtacg agtacttcac
     3601 cgtctacaac gagctcacga aggtgaagta cgtcaccgag ggcatgcgca agccagcgtt
     3661 cctgtccggg gagcagaaga aggctatcgt ggacctcctg ttcaagacca accggaaggt
     3721 cacggttaag caactcaagg aggactactt caagaagatc gagtgcttcg attcggtcga
     3781 gatcagcggc gttgaggacc gcttcaacgc cagcctcggg acctaccacg atctcctgaa
     3841 gatcatcaag gataaggact tcctggacaa cgaggagaac gaggatatcc tggaggacat
     3901 cgtgctgacc ctcacgctgt tcgaggacag ggagatgatc gaggagcgcc tgaagacgta
     3961 cgcccatctc ttcgatgaca aggtcatgaa gcaactcaag cgccggagat acaccggctg
     4021 ggggaggctg tcccgcaagc tcatcaacgg catccgggac aagcagtccg ggaagaccat
     4081 cctcgacttc ctcaagagcg atggcttcgc caacaggaac ttcatgcaac tgatccacga
     4141 tgacagcctc accttcaagg aggatatcca aaaggctcaa gtgagcggcc agggggactc
     4201 gctgcacgag catatcgcga acctcgctgg ctcccccgcg atcaagaagg gcatcctcca
     4261 gaccgtgaag gttgtggacg agctcgtgaa ggtcatgggc cggcacaagc ctgagaacat
     4321 cgtcatcgag atggccagag agaaccaaac cacgcagaag gggcaaaaga actctaggga
     4381 gcgcatgaag cgcatcgagg agggcatcaa ggagctgggg tcccaaatcc tcaaggagca
     4441 cccagtggag aacacccaac tgcagaacga gaagctctac ctgtactacc tccagaacgg
     4501 cagggatatg tacgtggacc aagagctgga tatcaaccgc ctcagcgatt acgacgtcga
     4561 tgccatcgtt ccccagtctt tcctgaagga tgactccatc gacaacaagg tcctcaccag
     4621 gtcggacaag aaccgcggca agtcagataa cgttccatct gaggaggtcg ttaagaagat
     4681 gaagaactac tggaggcagc tcctgaacgc caagctgatc acgcaaagga agttcgacaa
     4741 cctcaccaag gctgagagag gcgggctctc agagctggac aaggccggct tcatcaagcg
     4801 gcagctggtc gagaccagac aaatcacgaa gcacgttgcg caaatcctcg actctcggat
     4861 gaacacgaag tacgatgaga acgacaagct gatcagggag gttaaggtga tcaccctgaa
     4921 gtctaagctc gtctccgact tcaggaagga tttccagttc tacaaggttc gcgagatcaa
     4981 caactaccac catgcccatg acgcttacct caacgctgtg gtcggcaccg ctctgatcaa
     5041 gaagtaccca aagctggagt ccgagttcgt gtacggggac tacaaggttt acgatgtgcg
     5101 caagatgatc gccaagtcgg agcaagagat cggcaaggct accgccaagt acttcttcta
     5161 ctcaaacatc atgaacttct tcaagaccga gatcacgctg gccaacggcg agatccggaa
     5221 gagaccgctc atcgagacca acggcgagac gggggagatc gtgtgggaca agggcaggga
     5281 tttcgcgacc gtccgcaagg ttctctccat gccccaggtg aacatcgtca agaagaccga
     5341 ggtccaaacg ggcgggttct caaaggagtc tatcctgcct aagcggaaca gcgacaagct
     5401 catcgccaga aagaaggact gggacccaaa gaagtacggc gggttcctct ggcctaccgt
     5461 ggcctactcg gtcctggttg tggcgaaggt tgagaagggc aagtccaaga agctcaagag
     5521 cgtgaaggag ctcctgggga tcaccatcat ggagaggtcc agcttcgaga agaacccaat
     5581 cgacttcctg gaggccaagg gctacaagga ggtgaagaag gacctgatca tcaagctccc
     5641 gaagtactct ctcttcgagc tggagaacgg caggaagaga atgctggctt ccgctaagca
     5701 gctccagaag gggaacgagc tcgcgctgcc aagcaagtac gtgaacttcc tctacctggc
     5761 ttcccactac gagaagctca agggcagccc ggaggacaac gagcaaaagc agctgttcgt
     5821 cgagcagcac aagcattacc tcgacgagat catcgagcaa atctccgagt tcagcaagcg
     5881 cgtgatcctc gccgacgcga acctggataa ggtcctctcc gcctacaaca agcaccggga
     5941 caagcccatc agagagcaag cggagaacat catccatctc ttcaccctga cgaacctcgg
     6001 cgctcctgct gctttcaagt acttcgacac cacgatcgat cggaagcaat acaggtccac
     6061 gaaggaggtc ctggacgcga ccctcatcca ccagtcgatc accggcctgt acgagacgag
     6121 gatcgacctc tcacaactcg gcggggataa gagacccgca gcaaccaaga aggcagggca
     6181 agcaaagaag aagaagacgc gtagcggagg atcttccgga ggatctagcg gctccgagac
     6241 accaggaaca tccgaaagcg ctacaccaga atctagcgga ggctcttccg gaggatctag
     6301 gcctaccctc aacatcgagg atgagtatcg cctccacgaa acctccaaag aaccggacgt
     6361 gtccctcggc agcacatggc tcagcgactt cccacaagcg tgggccgaaa ccggcggcat
     6421 gggcctcgcc gtccgccaag ccccactcat tatcccgctg aaggcgacct ccacaccggt
     6481 gtccatcaag cagtacccga tgagccaaga ggcgaggctc gggattaagc cgcacattca
     6541 gcgcctcctc gatcaaggca ttctcgtgcc gtgccaatcc ccgtggaata caccactcct
     6601 cccggtcaaa aagccgggca ccaacgacta tcgcccggtc caagatctcc gcgaggtcaa
     6661 caagcgcgtg gaagacatcc acccgaccgt cccgaacccg tataatctgc tctccgggct
     6721 cccaccatcc caccagtggt atacagtgct ggacctcaaa gacgccttct tctgtctccg
     6781 cctccaccca acaagccagc cgctcttcgc cttcgagtgg cgcgacccgg agatgggcat
     6841 ctccggccaa ctgacatgga cacgcctccc gcaaggcttc aagaacagcc cgacactctt
     6901 caacgaggcg ctccataggg acctcgcgga ttttcgcatc cagcatccgg acctcatcct
     6961 cctccagtat gtggatgatc tcctcctcgc cgcgacctcc gagctggatt gtcaacaagg
     7021 cacacgcgcg ctcctccaaa cactcgggaa cctcggctat cgcgcgtccg cgaaaaaggc
     7081 ccaaatctgc cagaagcaag tgaagtacct cgggtatctg ctcaaggaag gccaacgctg
     7141 gctcaccgaa gcgcgcaaag aaacagtgat ggggcaaccg acaccgaaaa caccacgcca
     7201 gctgcgcgag tttctcggca aagccggctt ctgtcgcctc ttcatcccgg gctttgccga
     7261 gatggccgcg ccactctacc cactcaccaa gccgggcaca ctgtttaact gggggccgga
     7321 tcagcagaaa gcctaccaag agatcaaaca agcgctcctc accgccccag cgctcgggct
     7381 cccagatctc acaaagccgt tcgagctgtt cgtcgatgag aagcaaggct acgcgaaggg
     7441 cgtgctcaca cagaagctcg gcccgtggag gaggccagtg gcctatctct ccaaaaaact
     7501 cgatccagtg gccgccggct ggccaccgtg tctgcgcatg gtcgccgcga ttgccgtgct
     7561 cacaaaggat gccggcaaac tcacaatggg ccagccgctg gtgatcctcg cgccacatgc
     7621 cgtggaagcc ctcgtcaaac agccgccgga taggtggctc tccaatgcgc gcatgaccca
     7681 ttaccaagcg ctcctcctcg acaccgatcg cgtccagttc ggcccagtgg tcgccctcaa
     7741 tccggcgaca ctgctgccac tcccagagga gggcctccaa cacaactgtc tggatattct
     7801 cgcggaagcg catggcacaa ggccagacct cacagatcaa ccgctcccgg atgcggatca
     7861 cacatggtat accgacggct cctctctgct ccaagagggc caaaggaaag ccggcgccgc
     7921 ggtgaccaca gaaacagaag tgatctgggc caaggccctc ccagccggca catccgcgca
     7981 aagggcggaa ctcatcgcgc tcacacaagc cctcaagatg gccgagggca agaagctcaa
     8041 cgtctacaca gactcccgct atgccttcgc caccgcccac attcacggcg aaatctatag
     8101 gaggcgcggc tggctcacaa gcgaggggaa ggagatcaag aacaaggatg agatcctcgc
     8161 gctgctcaag gccctctttc tcccgaagcg cctctccatc atccactgtc cgggccacca
     8221 aaaggggcac tccgcggaag cgaggggcaa taggatggcc gatcaagccg cgcgcaaagc
     8281 cgcgattacc gaaaccccag acacatccac cctcctcatc gaaaactcct ccccaccgaa
     8341 gaagaaaagg aaggtgtgag aattcggtac gctgaaatca ccagtctctc tctacaaatc
     8401 tatctctctc tattttctcc ataaataatg tgtgagtagt ttcccgataa gggaaattag
     8461 ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt
     8521 atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa
     8581 aatccagatc tcctaaagtc cctatagatc tttgtcgtga atataaacca gacacgagac
     8641 gactaaacct ggagcccaga cgccgttcga agctagaagt accgcttagg caggaggccg
     8701 ttagggaaaa gatgctaagg cagggttggt tacgttgact cccccgtagg tttggtttaa
     8761 atatgatgaa gtggacggaa ggaaggagga agacaaggaa ggataaggtt gcaggccctg
     8821 tgcaaggtaa gaagatggaa atttgataga ggtacgctac tatacttata ctatacgcta
     8881 agggaatgct tgtatttata ccctataccc cctaataacc ccttatcaat ttaagaaata
     8941 atccgcataa gcccccgctt aaaaattggt atcagagcca tgaataggtc tatgaccaaa
     9001 actcaagagg ataaaacctc accaaaatac gaaagagttc ttaactctaa agataaaaga
     9061 tctttcaaga tcaaaactag ttccctcaca ccggtgacgg ggatcgcatg cgatatctcg
     9121 agatctagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9181 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9241 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9301 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9361 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9421 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9481 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9541 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9601 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9661 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9721 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9781 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9841 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9901 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9961 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10021 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10081 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10141 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10201 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10261 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10321 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10381 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10441 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10501 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10561 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10621 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10681 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10741 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10801 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10861 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10921 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10981 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11041 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11101 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11161 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11221 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11281 cgaaaagtgc cacctgccag tgccaagcta attc
//
