LOCUS       sequence-333607-       19919 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in rice plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19919)
  AUTHORS   Lin Q, Jin S, Zong Y, Yu H, Zhu Z, Liu G, Kou L, Wang Y, Qiu JL, Li 
            J, Gao C
  TITLE     High-efficiency prime editing with optimized, paired pegRNAs in 
            plants.
  JOURNAL   Nat Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 
            10.1038/s41587-021-00868-w.
  PUBMED    33767395
REFERENCE   2  (bases 1 to 19919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: 
            10.1038/s41587-021-00868-w."
COMMENT     Sequence Label: pH-nCas9-PPE-V2
FEATURES             Location/Qualifiers
     source          1..19919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        331..710
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             990..1781
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1933..2008
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2312..4304
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             4315..4335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(4336..6849,6850..6852,6853..8436)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8349..8357
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8443..8463
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10615..10635
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11294..11310)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11294..11310)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11307..11329)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11318..11334
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11342..11348,11349..11366,11367..11372))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11387..11408
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        11599..12275
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12212..12232
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12342..13367
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13408..13582
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    13660..13684
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14109..14903
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14990..15578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15479..15498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15732..15749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15764..15904
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15830..15849)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15990..16012
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16248..16442
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(16508..17581)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18010..18639)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgcctta taaagtaatt catccaggtc accaagttct
      301 aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac
      361 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
      421 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
      481 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
      541 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
      601 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
      661 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa
      721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      961 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1801 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1861 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1921 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1981 acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc
     2041 gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc
     2101 agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc
     2161 gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc
     2221 cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg
     2281 atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
     2341 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     2401 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     2461 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     2521 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2581 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2641 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2701 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2761 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2821 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2881 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2941 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     3001 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     3061 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     3121 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     3181 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     3241 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     3301 cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatggt
     3361 tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc
     3421 cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa
     3481 cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat
     3541 cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt
     3601 caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt
     3661 gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct gtttcaaact
     3721 acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg
     3781 aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt
     3841 tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg
     3901 ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt
     3961 tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg
     4021 atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac
     4081 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat
     4141 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc
     4201 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt
     4261 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcagccctag gatgcctaag
     4321 aaaaagagaa aagtggacaa gaagtactcg atcggcctcg atattgggac taactctgtt
     4381 ggctgggccg tgatcaccga cgagtacaag gtgccctcaa agaagttcaa ggtcctgggc
     4441 aacaccgatc ggcattccat caagaagaat ctcattggcg ctctcctgtt cgacagcggc
     4501 gagacggctg aggctacgcg gctcaagcgc accgcccgca ggcggtacac gcgcaggaag
     4561 aatcgcatct gctacctgca ggagattttc tccaacgaga tggcgaaggt tgacgattct
     4621 ttcttccaca ggctggagga gtcattcctc gtggaggagg ataagaagca cgagcggcat
     4681 ccaatcttcg gcaacattgt cgacgaggtt gcctaccacg agaagtaccc tacgatctac
     4741 catctgcgga agaagctcgt ggactccaca gataaggcgg acctccgcct gatctacctc
     4801 gctctggccc acatgattaa gttcaggggc catttcctga tcgaggggga tctcaacccg
     4861 gacaatagcg atgttgacaa gctgttcatc cagctcgtgc agacgtacaa ccagctcttc
     4921 gaggagaacc ccattaatgc gtcaggcgtc gacgcgaagg ctatcctgtc cgctaggctc
     4981 tcgaagtctc ggcgcctcga gaacctgatc gcccagctgc cgggcgagaa gaagaacggc
     5041 ctgttcggga atctcattgc gctcagcctg gggctcacgc ccaacttcaa gtcgaatttc
     5101 gatctcgctg aggacgccaa gctgcagctc tccaaggaca catacgacga tgacctggat
     5161 aacctcctgg cccagatcgg cgatcagtac gcggacctgt tcctcgctgc caagaatctg
     5221 tcggacgcca tcctcctgtc tgatattctc agggtgaaca ccgagattac gaaggctccg
     5281 ctctcagcct ccatgatcaa gcgctacgac gagcaccatc aggatctgac cctcctgaag
     5341 gcgctggtca ggcagcagct ccccgagaag tacaaggaga tcttcttcga tcagtcgaag
     5401 aacggctacg ctgggtacat tgacggcggg gcctctcagg aggagttcta caagttcatc
     5461 aagccgattc tggagaagat ggacggcacg gaggagctgc tggtgaagct caatcgcgag
     5521 gacctcctga ggaagcagcg gacattcgat aacggcagca tcccacacca gattcatctc
     5581 ggggagctgc acgctatcct gaggaggcag gaggacttct accctttcct caaggataac
     5641 cgcgagaaga tcgagaagat tctgactttc aggatcccgt actacgtcgg cccactcgct
     5701 aggggcaact cccgcttcgc ttggatgacc cgcaagtcag aggagacgat cacgccgtgg
     5761 aacttcgagg aggtggtcga caagggcgct agcgctcagt cgttcatcga gaggatgacg
     5821 aatttcgaca agaacctgcc aaatgagaag gtgctcccta agcactcgct cctgtacgag
     5881 tacttcacag tctacaacga gctgactaag gtgaagtatg tgaccgaggg catgaggaag
     5941 ccggctttcc tgtctgggga gcagaagaag gccatcgtgg acctcctgtt caagaccaac
     6001 cggaaggtca cggttaagca gctcaaggag gactacttca agaagattga gtgcttcgat
     6061 tcggtcgaga tctctggcgt tgaggaccgc ttcaacgcct ccctggggac ctaccacgat
     6121 ctcctgaaga tcattaagga taaggacttc ctggacaacg aggagaatga ggatatcctc
     6181 gaggacattg tgctgacact cactctgttc gaggaccggg agatgatcga ggagcgcctg
     6241 aagacttacg cccatctctt cgatgacaag gtcatgaagc agctcaagag gaggaggtac
     6301 accggctggg ggaggctgag caggaagctc atcaacggca ttcgggacaa gcagtccggg
     6361 aagacgatcc tcgacttcct gaagagcgat ggcttcgcga accgcaattt catgcagctg
     6421 attcacgatg acagcctcac attcaaggag gatatccaga aggctcaggt gagcggccag
     6481 ggggactcgc tgcacgagca tatcgcgaac ctcgctggct cgccagctat caagaagggg
     6541 attctgcaga ccgtgaaggt tgtggacgag ctggtgaagg tcatgggcag gcacaagcct
     6601 gagaacatcg tcattgagat ggcccgggag aatcagacca cgcagaaggg ccagaagaac
     6661 tcacgcgaga ggatgaagag gatcgaggag ggcattaagg agctggggtc ccagatcctc
     6721 aaggagcacc cggtggagaa cacgcagctg cagaatgaga agctctacct gtactacctc
     6781 cagaatggcc gcgatatgta tgtggaccag gagctggata ttaacaggct cagcgattac
     6841 gacgtcgatg ccatcgttcc acagtcattc ctgaaggatg actccattga caacaaggtc
     6901 ctcaccaggt cggacaagaa ccggggcaag tctgataatg ttccttcaga ggaggtcgtt
     6961 aagaagatga agaactactg gcgccagctc ctgaatgcca agctgatcac gcagcggaag
     7021 ttcgataacc tcacaaaggc tgagaggggc gggctctctg agctggacaa ggcgggcttc
     7081 atcaagaggc agctggtcga gacacggcag atcactaagc acgttgcgca gattctcgac
     7141 tcacggatga acactaagta cgatgagaat gacaagctga tccgcgaggt gaaggtcatc
     7201 accctgaagt caaagctcgt ctccgacttc aggaaggatt tccagttcta caaggttcgg
     7261 gagatcaaca attaccacca tgcccatgac gcgtacctga acgcggtggt cggcacagct
     7321 ctgatcaaga agtacccaaa gctcgagagc gagttcgtgt acggggacta caaggtttac
     7381 gatgtgagga agatgatcgc caagtcggag caggagattg gcaaggctac cgccaagtac
     7441 ttcttctact ctaacattat gaatttcttc aagacagaga tcactctggc caatggcgag
     7501 atccggaagc gccccctcat cgagacgaac ggcgagacgg gggagatcgt gtgggacaag
     7561 ggcagggatt tcgcgaccgt caggaaggtt ctctccatgc cacaagtgaa tatcgtcaag
     7621 aagacagagg tccagactgg cgggttctct aaggagtcaa ttctgcctaa gcggaacagc
     7681 gacaagctca tcgcccgcaa gaaggactgg gatccgaaga agtacggcgg gttcgacagc
     7741 cccactgtgg cctactcggt cctggttgtg gcgaaggttg agaagggcaa gtccaagaag
     7801 ctcaagagcg tgaaggagct gctggggatc acgattatgg agcgctccag cttcgagaag
     7861 aacccgatcg atttcctgga ggcgaagggc tacaaggagg tgaagaagga cctgatcatt
     7921 aagctcccca agtactcact cttcgagctg gagaacggca ggaagcggat gctggcttcc
     7981 gctggcgagc tgcagaaggg gaacgagctg gctctgccgt ccaagtatgt gaacttcctc
     8041 tacctggcct cccactacga gaagctcaag ggcagccccg aggacaacga gcagaagcag
     8101 ctgttcgtcg agcagcacaa gcattacctc gacgagatca ttgagcagat ttccgagttc
     8161 tccaagcgcg tgatcctggc cgacgcgaat ctggataagg tcctctccgc gtacaacaag
     8221 caccgcgaca agccaatcag ggagcaggct gagaatatca ttcatctctt caccctgacg
     8281 aacctcggcg cccctgctgc tttcaagtac ttcgacacaa ctatcgatcg caagaggtac
     8341 acaagcacta aggaggtcct ggacgcgacc ctcatccacc agtcgattac cggcctctac
     8401 gagacgcgca tcgacctgtc tcagctcggg ggcgacgaat tcccaaagaa gaagcggaag
     8461 gtggagctca gcggaggatc ttccggagga tctagcggct ccgagacacc aggaacatcc
     8521 gaaagcgcta caccagaatc tagcggaggc tcttccggag gatctaggcc taccctcaac
     8581 atcgaggatg agtatcgcct ccacgaaacc tccaaagaac cggacgtgtc cctcggcagc
     8641 acatggctca gcgacttccc acaagcgtgg gccgaaaccg gcggcatggg cctcgccgtc
     8701 cgccaagccc cactcattat cccgctgaag gcgacctcca caccggtgtc catcaagcag
     8761 tacccgatga gccaagaggc gaggctcggg attaagccgc acattcagcg cctcctcgat
     8821 caaggcattc tcgtgccgtg ccaatccccg tggaatacac cactcctccc ggtcaaaaag
     8881 ccgggcacca acgactatcg cccggtccaa gatctccgcg aggtcaacaa gcgcgtggaa
     8941 gacatccacc cgaccgtccc gaacccgtat aatctgctct ccgggctccc accatcccac
     9001 cagtggtata cagtgctgga cctcaaagac gccttcttct gtctccgcct ccacccaaca
     9061 agccagccgc tcttcgcctt cgagtggcgc gacccggaga tgggcatctc cggccaactg
     9121 acatggacac gcctcccgca aggcttcaag aacagcccga cactcttcaa cgaggcgctc
     9181 catagggacc tcgcggattt tcgcatccag catccggacc tcatcctcct ccagtatgtg
     9241 gatgatctcc tcctcgccgc gacctccgag ctggattgtc aacaaggcac acgcgcgctc
     9301 ctccaaacac tcgggaacct cggctatcgc gcgtccgcga aaaaggccca aatctgccag
     9361 aagcaagtga agtacctcgg gtatctgctc aaggaaggcc aacgctggct caccgaagcg
     9421 cgcaaagaaa cagtgatggg gcaaccgaca ccgaaaacac cacgccagct gcgcgagttt
     9481 ctcggcaaag ccggcttctg tcgcctcttc atcccgggct ttgccgagat ggccgcgcca
     9541 ctctacccac tcaccaagcc gggcacactg tttaactggg ggccggatca gcagaaagcc
     9601 taccaagaga tcaaacaagc gctcctcacc gccccagcgc tcgggctccc agatctcaca
     9661 aagccgttcg agctgttcgt cgatgagaag caaggctacg cgaagggcgt gctcacacag
     9721 aagctcggcc cgtggaggag gccagtggcc tatctctcca aaaaactcga tccagtggcc
     9781 gccggctggc caccgtgtct gcgcatggtc gccgcgattg ccgtgctcac aaaggatgcc
     9841 ggcaaactca caatgggcca gccgctggtg atcctcgcgc cacatgccgt ggaagccctc
     9901 gtcaaacagc cgccggatag gtggctctcc aatgcgcgca tgacccatta ccaagcgctc
     9961 ctcctcgaca ccgatcgcgt ccagttcggc ccagtggtcg ccctcaatcc ggcgacactg
    10021 ctgccactcc cagaggaggg cctccaacac aactgtctgg atattctcgc ggaagcgcat
    10081 ggcacaaggc cagacctcac agatcaaccg ctcccggatg cggatcacac atggtatacc
    10141 gacggctcct ctctgctcca agagggccaa aggaaagccg gcgccgcggt gaccacagaa
    10201 acagaagtga tctgggccaa ggccctccca gccggcacat ccgcgcaaag ggcggaactc
    10261 atcgcgctca cacaagccct caagatggcc gagggcaaga agctcaacgt ctacacagac
    10321 tcccgctatg ccttcgccac cgcccacatt cacggcgaaa tctataggag gcgcggctgg
    10381 ctcacaagcg aggggaagga gatcaagaac aaggatgaga tcctcgcgct gctcaaggcc
    10441 ctctttctcc cgaagcgcct ctccatcatc cactgtccgg gccaccaaaa ggggcactcc
    10501 gcggaagcga ggggcaatag gatggccgat caagccgcgc gcaaagccgc gattaccgaa
    10561 accccagaca catccaccct cctcatcgaa aactcctccc caagcggcgg cagcccgaag
    10621 aagaaaagga aggtgtgaga gctcagagct ttcgttcgta tcatcggttt cgacaacgtt
    10681 cgtcaagttc aatgcatcag tttcattgcg cacacaccag aatcctactg agtttgagta
    10741 ttatggcatt gggaaaactg tttttcttgt accatttgtt gtgcttgtaa tttactgtgt
    10801 tttttattcg gttttcgcta tcgaactgtg aaatggaaat ggatggagaa gagttaatga
    10861 atgatatggt ccttttgttc attctcaaat taatattatt tgttttttct cttatttgtt
    10921 gtgtgttgaa tttgaaatta taagagatat gcaaacattt tgttttgagt aaaaatgtgt
    10981 caaatcgtgg cctctaatga ccgaagttaa tatgaggagt aaaacacttg tagttgtacc
    11041 attatgctta ttcactaggc aacaaatata ttttcagacc tagaaaagct gcaaatgtta
    11101 ctgaatacaa gtatgtcctc ttgtgtttta gacatttatg aactttcctt tatgtaattt
    11161 tccagaatcc ttgtcagatt ctaatcattg ctttataatt atagttatac tcatggattt
    11221 gtagttgagt atgaaaatat tttttaatgc attttatgac ttgccaattg attgacaacg
    11281 aattcgtaat catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
    11341 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
    11401 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    11461 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggc tagagcagct
    11521 tgccaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
    11581 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
    11641 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
    11701 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
    11761 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
    11821 cacgtcttca aagcaagtgg attgatgtga acatggtgga gcacgacact ctcgtctact
    11881 ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact tttcaacaaa
    11941 gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac ttcatcaaaa
    12001 ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa ggaaaggcta
    12061 tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca
    12121 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct
    12181 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat
    12241 aaggaagttc atttcatttg gagaggacac gctgaaatca ccagtctctc tctacaaatc
    12301 tatctctctc gagctttcgc agatccgggg ggcaatgaga tatgaaaaag cctgaactca
    12361 ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc gacctgatgc
    12421 agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg
    12481 tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt tatcggcact
    12541 ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggagttt agcgagagcc
    12601 tgacctattg catctcccgc cgttcacagg gtgtcacgtt gcaagacctg cctgaaaccg
    12661 aactgcccgc tgttctacaa ccggtcgcgg aggctatgga tgcgatcgct gcggccgatc
    12721 ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa tacactacat
    12781 ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa actgtgatgg
    12841 acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg
    12901 actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg
    12961 acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg gattcccaat
    13021 acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag cagcagacgc
    13081 gctacttcga gcggaggcat ccggagcttg caggatcgcc acgactccgg gcgtatatgc
    13141 tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc gatgatgcag
    13201 cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact gtcgggcgta
    13261 cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg
    13321 atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa gaaatagagt agatgccgac
    13381 cgggatctgt cgatcgacaa gctcgagttt ctccataata atgtgtgagt agttcccaga
    13441 taagggaatt agggttccta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta
    13501 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa
    13561 atccagtact aaaatccaga tcccccgaat taattcggcg ttaattcagt acattaaaaa
    13621 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
    13681 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
    13741 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
    13801 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
    13861 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
    13921 caccgcggtt tcaaaatcgg ctccgtcgat actatgttat acgccaactt tgaaaacaac
    13981 tttgaaaaag ctgttttctg gtatttaagg ttttagaatg caaggaacag tgaattggag
    14041 ttcgtcttgt tataattagc ttcttggggt atctttaaat actgtagaaa agaggaagga
    14101 aataataaat ggctaaaatg agaatatcac cggaattgaa aaaactgatc gaaaaatacc
    14161 gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg gtgggagaaa
    14221 atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc tatgatgtgg
    14281 aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca aaggtcctgc
    14341 actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat ggcgtccttt
    14401 gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt
    14461 gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat agcttagaca
    14521 gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg gattgcgaaa
    14581 actgggaaga agacactcca tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg
    14641 aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc aacatctttg
    14701 tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg gcggacaagt
    14761 ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa gaacagtatg
    14821 tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata aaatattata
    14881 ttttactgga tgaattgttt tagtacctag aatgcatgac caaaatccct taacgtgagt
    14941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    15001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    15061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    15121 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    15181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    15241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    15301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    15361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    15421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    15481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    15541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
    15601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
    15661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
    15721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
    15781 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
    15841 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
    15901 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
    15961 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
    16021 gtcatcaccg aaacgcgcga ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg
    16081 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
    16141 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
    16201 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
    16261 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
    16321 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
    16381 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
    16441 caggaaacag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
    16501 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
    16561 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
    16621 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
    16681 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
    16741 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
    16801 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
    16861 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
    16921 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
    16981 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
    17041 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
    17101 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
    17161 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
    17221 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
    17281 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
    17341 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
    17401 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
    17461 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
    17521 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
    17581 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
    17641 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
    17701 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
    17761 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
    17821 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
    17881 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
    17941 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
    18001 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
    18061 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
    18121 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
    18181 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
    18241 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
    18301 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
    18361 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
    18421 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
    18481 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
    18541 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
    18601 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
    18661 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
    18721 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
    18781 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
    18841 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
    18901 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
    18961 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
    19021 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
    19081 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
    19141 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
    19201 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
    19261 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
    19321 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
    19381 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
    19441 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
    19501 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
    19561 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
    19621 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
    19681 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
    19741 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
    19801 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
    19861 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaa
//
