LOCUS       sequence-333623-        9949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS vector to express sfcherry2(1-10) in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Kamiyama R, Banzai K, Liu P, Marar A, Tamura R, Jiang F, Fitch MA, 
            Xie J, Kamiyama D
  TITLE     Cell-type-specific, multicolor labeling of endogenous proteins with 
            split fluorescent protein tags in Drosophila.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Jun 8;118(23). pii: 2024690118. doi: 
            10.1073/pnas.2024690118.
  PUBMED    34074768
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-06-8"; volume: "118(23). pii: 
            2024690118. doi"; pages: " 10.1073/pnas.2024690118"
COMMENT     Sequence Label: pACUH-sfcherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..9949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2434..2503
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(3046..3076,3077..3278)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    3359..3453
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    3477..3571
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        3590..3828
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3775..3796
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     primer_bind     complement(4001..4019)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     intron          4578..4643
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4773..4793
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5065..5199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5102..5121)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5156..5175
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5226..5244)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5704..5775,6170..6443,6518..7172,7234..7549,7770..7901,
                     7972..8586)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9364..9918,9919..9949)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tcgacatgcc cgccgtgacc gtcgagaacc cgctgacgct
     2281 gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg tgaccaccgc
     2341 gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg ggatcaacta
     2401 ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt acgcgcccgg
     2461 ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga cctacatcgt
     2521 cgatatgcgg tgtgaaatac cgcaccgaat cgcgcggaac taacgacagt cgctccaagg
     2581 tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg gagacagcga aagagcaact
     2641 acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg cgcgatttga aaagtatgta
     2701 tataaaaaat atatcccggt gttttatgta gcgataaacg agtttttgat gtaaggtatg
     2761 caggtgtgta agtcttttgg ttagaagaca aatccaaagt ctacttgtgg ggatgttcga
     2821 aggggaaata cttgtattct ataggtcata tcttgttttt attggcacaa atataattac
     2881 attagctttt tgagggggca ataaacagta aacacgatgg taataatggt aaaaaaaaaa
     2941 acaagcagtt atttcggata tatgtcggct actccttgcg tcgggcccga agtcttagag
     3001 ccagatatgc gagcacccgg aagctcacga tgagaatggc cagaccatga tgaaataaca
     3061 taaggtggtc ccgtcggcaa gagacatcca cttaacgtat gcttgcaata agtgcgagtg
     3121 aaaggaatag tattctgagt gtcgtattga gtctgagtga gacagcgata tgattgttga
     3181 ttaaccctta gcatgtccgt ggggtttgaa ttaactcata atattaatta gacgaaatta
     3241 tttttaaagt tttattttta ataatttgcg agtacgcaaa gcttggctgc atccaacgcg
     3301 ttgggagctc tccggatcca agctggccgc ggctcgatcc gcttgcatgc ctgcaggtcg
     3361 gagtactgtc ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag
     3421 tactgtcctc cgagcggagt actgtcctcc gagcggaagc ttgcatgcct gcaggtcgga
     3481 gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta
     3541 ctgtcctccg agcggagtac tgtcctccga gcggagactc tagcgagcgc cggagtataa
     3601 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg
     3661 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa
     3721 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact
     3781 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagggaattg
     3841 ggaattcgtt aacagatctg cggccgcatg gaggaggaca acatggccat catcaaggag
     3901 ttcatgagat tcaaggtgca catggagggc agcgtgaacg gccacgagtt cgagatcgag
     3961 ggcgagggcg agggccaccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     4021 ggcggccccc tgcccttcgc ctgggacatc ctgagccccc agttcatgta cggcagcaag
     4081 gcctacgtga agcaccccgc cgacatcccc gactacctga agctgagctt ccccgagggc
     4141 ttcacctggg agagagtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     4201 agcagcctgc aggacggcca gttcatctac aaggtgaagc tgctgggcat caacttcccc
     4261 agcgacggcc ccgtgatgca gaagaagacc atgggctggg aggccagcac cgagagaatg
     4321 taccccgagg acggcgccct gaagggcgag atcaaccaga gactgaagct gaaggacggc
     4381 ggccactacg acgccgaggt gaagaccacc tacaaggcca agaagcccgt gcagctgccc
     4441 ggcgcctaca acgtggacat caagctggac atcaccagcc acaacgagga ctgatctaga
     4501 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     4561 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     4621 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     4681 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     4741 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     4801 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     4861 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     4921 ttatggaaaa atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     4981 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5041 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5101 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5161 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc actagaaggc ctaattctag
     5221 tatgtatgta agttaataaa accctttttt ggagaatgta gatttaaaaa aacatatttt
     5281 ttttttattt tttactgcac tggacatcat tgaacttatc tgatcagttt taaatttact
     5341 tcgatccaag ggtatttgaa gtaccaggtt ctttcgatta cctctcactc aaaatgacat
     5401 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
     5461 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacctag cgtcaatgtc
     5521 cgccttcagt tgcactttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     5581 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     5641 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     5701 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     5761 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     5821 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     5881 aaaccgcaat cgcacaaaca aatagtgaca cgaaacagat tattctggta gctgtgctcg
     5941 ctatataaga caatttttaa gatcatatca tgatcaagac atctaaaggc attcattttc
     6001 gactacattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     6061 acaaaaaata aataaaagta taaacctact tcgtaggata cttcgttttg ttcggggtta
     6121 gatgagcata acgcttgtag ttgatatttg agatccccta tcattgcagg gtgacagcgg
     6181 agcggcttcg cagagctgca ttaaccaggg cttcgggcag gccaaaaact acggcacgct
     6241 cctgccaccc agtccgccgg aggactccgg ttcagggagc ggccaactag ccgagaacct
     6301 cacctatgcc tggcacaata tggacatctt tggggcggtc aatcagccgg gctccggatg
     6361 gcggcagctg gtcaaccgga cacgcggact attctgcaac gagcgacaca taccggcgcc
     6421 caggaaacat ttgctcaaga acggtgagtt tctattcgca gtcggctgat ctgtgtgaaa
     6481 tcttaataaa gggtccaatt accaatttga aactcagttt gcggcgtggc ctatccgggc
     6541 gaacttttgg ccgtgatggg cagttccggt gccggaaaga cgaccctgct gaatgccctt
     6601 gcctttcgat cgccgcaggg catccaagta tcgccatccg ggatgcgact gctcaatggc
     6661 caacctgtgg acgccaagga gatgcaggcc aggtgcgcct atgtccagca ggatgacctc
     6721 tttatcggct ccctaacggc cagggaacac ctgattttcc aggccatggt gcggatgcca
     6781 cgacatctga cctatcggca gcgagtggcc cgcgtggatc aggtgatcca ggagctttcg
     6841 ctcagcaaat gtcagcacac gatcatcggt gtgcccggca gggtgaaagg tctgtccggc
     6901 ggagaaagga agcgtctggc attcgcctcc gaggcactaa ccgatccgcc gcttctgatc
     6961 tgcgatgagc ccacctccgg actggactca tttaccgccc acagcgtcgt ccaggtgctg
     7021 aagaagctgt cgcagaaggg caagaccgtc atcctgacca ttcatcagcc gtcttccgag
     7081 ctgtttgagc tctttgacaa gatccttctg atggccgagg gcagggtagc tttcttgggc
     7141 actcccagcg aagccgtcga cttcttttcc tagtgagttc gatgtgttta ttaagggtat
     7201 ctagcattac attacatctc aactcctatc cagcgtgggt gcccagtgtc ctaccaacta
     7261 caatccggcg gacttttacg tacaggtgtt ggccgttgtg cccggacggg agatcgagtc
     7321 ccgtgatcgg atcgccaaga tatgcgacaa ttttgctatt agcaaagtag cccgggatat
     7381 ggagcagttg ttggccacca aaaatttgga gaagccactg gagcagccgg agaatgggta
     7441 cacctacaag gccacctggt tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc
     7501 ggtgctcaag gaaccactcc tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc
     7561 cagtggaaac aaatgatata acgcttacaa ttcttggaaa caaattcgct agattttagt
     7621 tagaattgcc tgattccaca cccttcttag tttttttcaa tgagatgtat agtttatagt
     7681 tttgcagaaa ataaataaat ttcatttaac tcgcgaacat gttgaagata tgaatattaa
     7741 tgagatgcga gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt
     7801 tgggccaaca actcacgcaa gtgggcgtga tgaatatcaa cggagccatc ttcctcttcc
     7861 tgaccaacat gacctttcaa aacgtctttg ccacgataaa tgtaagtctt gtttagaata
     7921 catttgcata ttaataattt actaactttc taatgaatcg attcgattta ggtgttcacc
     7981 tcagagctgc cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac
     8041 tttctgggca aaacgattgc cgaattaccg ctttttctca cagtgccact ggtcttcacg
     8101 gcgattgcct atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg
     8161 gcgctggtca ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc
     8221 agctcctcga cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc
     8281 tttggcggct tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac
     8341 ctctcatggt tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag
     8401 ccgggcgaaa ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc
     8461 ctggagacgc ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt
     8521 ctcatcgtga gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag
     8581 gagtagccga catatatccg aaataactgc ttgttttttt ttttaccatt attaccatcg
     8641 tgtttactgt ttattgcccc ctcaaaaagc taatgtaatt atatttgtgc caataaaaac
     8701 aagatatgac ctatagaata caagtatttc cccttcgaac atccccacaa gtagactttg
     8761 gatttgtctt ctaaccaaaa gacttacaca cctgcatacc ttacatcaaa aactcgttta
     8821 tcgctacata aaacaccggg atatattttt tatatacata cttttcaaat cgcgcgccct
     8881 cttcataatt cacctccacc acaccacgtt tcgtagttgc tctttcgctg tctcccaccc
     8941 gctctccgca acacattcac cttttgttcg acgaccttgg agcgactgtc gttagttccg
     9001 cgcgattcgg ttcgctcaaa tggttccgag tggttcattt cgtctcaata gaaattagta
     9061 ataaatattt gtatgtacaa tttatttgct ccaatatatt tgtatatatt tccctcacag
     9121 ctatatttat tctaatttaa tattatgact ttttaaggta attttttgtg acctgttcgg
     9181 agtgattagc gttacaattt gaactgaaag tgacatccag tgtttgttcc ttgtgtagat
     9241 gcatctcaaa aaaatggtgg gcataatagt gttgtttata tatatcaaaa ataacaacta
     9301 taataataag aatacattta atttagaaaa tgcttggatt tcactggaac tagaattaat
     9361 tcggctgctg ctctaaacga cgcatttcgt actccaaagt acgaattttt tccctcaagc
     9421 tcttattttc attaaacaat gaacaggacc taacgcacag tcacgttatt gtttacataa
     9481 atgatttttt ttactattca aacttactct gtttgtgtac tcccactggt atagccttct
     9541 tttatctttt ctggttcagg ctctatcact ttactaggta cggcatctgc gttgagtcgc
     9601 ctccttttaa atgtctgacc ttttgcaggt gcagccttcc actgcgaatc attaaagtgg
     9661 gtatcacaaa tttgggagtt ttcaccaagg ctgcacccaa ggctctgctc ccacaatttt
     9721 ctcttaatag cacacttcgg cacgtgaatt aattttactc cagtcacagc tttgcagcaa
     9781 aatttgcaat atttcatttt tttttattcc acgtaagggt taatgttttc aaaaaaaaat
     9841 tcgtccgcac acaacctttc ctctcaacaa gcaaacgtgc actgaattta agtgtatact
     9901 tcggtaagct tcggctatcg acgggaccac cttatgttat ttcatcatg
//
