LOCUS       sequence-333633-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAR-AD1.A5 part (C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pC-02
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     482..501
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     674..691
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1019..1038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1020..1038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1049..1066)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1049..1065)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1058..1080)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1381..1485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1486..1554,1555..2346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1704..1723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2517..2524,1..581)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
       61 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
      121 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
      181 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
      241 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
      301 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
      361 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
      421 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
      481 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
      541 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
      601 ttttacggtt cctggccttt tgctggcctt ttgctcatta ggcaccccag gctttacccg
      661 aacgaccgag cgcagcgagt cagtgagcga ggaagcggag agcgcccaat acgcaaggaa
      721 acagctatga ccatgttaat gcagctggca cgacaggttt cccgactgga aagcgggcag
      781 tgagcggaag gcccatgagg ccagtcttgt gctccaggta ccgtccccat ccaactcaac
      841 caagatgggt acaacgcctg taaacagctc ctctccttta ctggtctcaa agcagttttt
      901 acagtgaatt gttttaatta gttgtataaa tgttggagca gcggggaatg tatacagttc
      961 aaccttgaga ccgagctctg gacaagacac gtggccttga cggccttccg ccaattcgcc
     1021 ctatagtgag tcgtattacg tcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     1081 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     1141 gtaatagcga agaggcccgc accgaaacgc ccttcccaac agttgcgcag cctgaatggc
     1201 gaatgggagc gccctgtagc ggccactcaa ccctatctcg gtctattctt ttgatttata
     1261 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     1321 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     1381 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     1441 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     1501 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     1561 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     1621 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     1681 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     1741 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     1801 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     1861 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     1921 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     1981 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     2041 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     2101 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     2161 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gttctcgcgg tatcattgca
     2221 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     2281 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     2341 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     2401 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     2461 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     2521 gatc
//
