LOCUS       sequence-333637-       10556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hTERT cDNA and p16 shRNA in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Smith JL, Lee LC, Read A, Li Q, Yu B, Lee CS, Luo J
  TITLE     One-step immortalization of primary human airway epithelial cells 
            capable of oncogenic transformation.
  JOURNAL   Cell Biosci. 2016 Nov 11;6:57. doi: 10.1186/s13578-016-0122-6. 
            eCollection 2016.
  PUBMED    27891214
REFERENCE   2  (bases 1 to 10556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Biosci."; date: "2016-11-11"; volume: "6:57. doi"; pages: " 
            10.1186/s13578-016-0122-6. eCollection 2016"
COMMENT     Sequence Label: MSCVpic2neo-hTERT-p16 shRNA
FEATURES             Location/Qualifiers
     source          1..10556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     ncRNA           1443..1564
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1592..1606
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           1634..1760
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        1827..2038
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2063..2089
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2093..5491
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRDC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     misc_feature    5508..6081
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5531..6083
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5675..5692)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5902..5921
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6099..6902
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6162..6181)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6772..6791
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             6949..7463
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7625..7641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7649..7655,7656..7673,7674..7679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7694..7715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7832..7849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8003..8591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8083..8102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8762..9553,9554..9622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9385..9404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9623..9727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9795..9813
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9851..9873)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9973..9992
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10186..10208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10336..10355
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(10555..10556,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ctagggataa cagggtaatt
     1441 gtttgaatga ggcttcagta ctttacagaa tcgttgcctg cacatcttgg aaacacttgc
     1501 tgggattact tcttcaggtt aacccaacag aaggctcgag aaggtatatt gctgttgaca
     1561 gtgagcgact cgggaaactt agatcatcat agtgaagcca cagatgtatg atgatctaag
     1621 tttcccgagg tgcctactgc ctcggaattc aaggggctac tttaggagca attatcttgt
     1681 ttactaaaac tgaatacctt gctatctctt tgatacattt ttacaaagct gaattaaaat
     1741 ggtataaatt aaatcacttt tttcaattgg aagactaatg cgtttaaaca cgcggcgacg
     1801 cgtcgatggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     1861 gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg
     1921 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     1981 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt
     2041 gtcgtgacgc gggccggcca tgtacccata cgatgttcca gattacgctg gtatgccgcg
     2101 cgctccccgc tgccgagccg tgcgctccct gctgcgcagc cactaccgcg aggtgctgcc
     2161 gctggccacg ttcgtgcggc gcctggggcc ccagggctgg cggctggtgc agcgcgggga
     2221 cccggcggct ttccgcgcgc tggtggccca gtgcctggtg tgcgtgccct gggacgcacg
     2281 gccgcccccc gccgccccct ccttccgcca ggtgtcctgc ctgaaggagc tggtggcccg
     2341 agtgctgcag aggctgtgcg agcgcggcgc gaagaacgtg ctggccttcg gcttcgcgct
     2401 gctggacggg gcccgcgggg gcccccccga ggccttcacc accagcgtgc gcagctacct
     2461 gcccaacacg gtgaccgacg cactgcgggg gagcggggcg tgggggctgc tgctgcgccg
     2521 cgtgggcgac gacgtgctgg ttcacctgct ggcacgctgc gcgctctttg tgctggtggc
     2581 tcccagctgc gcctaccagg tgtgcgggcc gccgctgtac cagctcggcg ctgccactca
     2641 ggcccggccc ccgccacacg ctagtggacc ccgaaggcgt ctgggatgcg aacgggcctg
     2701 gaaccatagc gtcagggagg ccggggtccc cctgggcctg ccagccccgg gtgcgaggag
     2761 gcgcgggggc agtgccagcc gaagtctgcc gttgcccaag aggcccaggc gtggcgctgc
     2821 ccctgagccg gagcggacgc ccgttgggca ggggtcctgg gcccacccgg gcaggacgcg
     2881 tggaccgagt gaccgtggtt tctgtgtggt gtcacctgcc agacccgccg aagaagccac
     2941 ctctttggag ggtgcgctct ctggcacgcg ccactcccac ccatccgtgg gccgccagca
     3001 ccacgcgggc cccccatcca catcgcggcc accacgtccc tgggacacgc cttgtccccc
     3061 ggtgtacgcc gagaccaagc acttcctcta ctcctcaggc gacaaggagc agctgcggcc
     3121 ctccttccta ctcagctctc tgaggcccag cctgactggc gctcggaggc tcgtggagac
     3181 catctttctg ggttccaggc cctggatgcc agggactccc cgcaggttgc cccgcctgcc
     3241 ccagcgctac tggcaaatgc ggcccctgtt tctggagctg cttgggaacc acgcgcagtg
     3301 cccctacggg gtgctcctca agacgcactg cccgctgcga gctgcggtca ccccagcagc
     3361 cggtgtctgt gcccgggaga agccccaggg ctctgtggcg gcccccgagg aggaggacac
     3421 agacccccgt cgcctggtgc agctgctccg ccagcacagc agcccctggc aggtgtacgg
     3481 cttcgtgcgg gcctgcctgc gccggctggt gcccccaggc ctctggggct ccaggcacaa
     3541 cgaacgccgc ttcctcagga acaccaagaa gttcatctcc ctggggaagc atgccaagct
     3601 ctcgctgcag gagctgacgt ggaagatgag cgtgcgggac tgcgcttggc tgcgcaggag
     3661 cccaggggtt ggctgtgttc cggccgcaga gcaccgtctg cgtgaggaga tcctggccaa
     3721 gttcctgcac tggctgatga gtgtgtacgt cgtcgagctg ctcaggtctt tcttttatgt
     3781 cacggagacc acgtttcaaa agaacaggct ctttttctac cggaagagtg tctggagcaa
     3841 gttgcaaagc attggaatca gacagcactt gaagagggtg cagctgcggg agctgtcgga
     3901 agcagaggtc aggcagcatc gggaagccag gcccgccctg ctgacgtcca gactccgctt
     3961 catccccaag cctgacgggc tgcggccgat tgtgaacatg gactacgtcg tgggagccag
     4021 aacgttccgc agagaaaaga gggccgagcg tctcacctcg agggtgaagg cactgttcag
     4081 cgtgctcaac tacgagcggg cgcggcgccc cggcctcctg ggcgcctctg tgctgggcct
     4141 ggacgatatc cacagggcct ggcgcacctt cgtgctgcgt gtgcgggccc aggacccgcc
     4201 gcctgagctg tactttgtca aggtggatgt gacgggcgcg tacgacacca tcccccagga
     4261 caggctcacg gaggtcatcg ccagcatcat caaaccccag aacacgtact gcgtgcgtcg
     4321 gtatgccgtg gtccagaagg ccgcccatgg gcacgtccgc aaggccttca agagccacgt
     4381 ctctaccttg acagacctcc agccgtacat gcgacagttc gtggctcacc tgcaggagac
     4441 cagcccgctg agggatgccg tcgtcatcga gcagagctcc tccctgaatg aggccagcag
     4501 tggcctcttc gacgtcttcc tacgcttcat gtgccaccac gccgtgcgca tcaggggcaa
     4561 gtcctacgtc cagtgccagg ggatcccgca gggctccatc ctctccacgc tgctctgcag
     4621 cctgtgctac ggcgacatgg agaacaagct gtttgcgggg attcggcggg acgggctgct
     4681 cctgcgtttg gtggatgatt tcttgttggt gacacctcac ctcacccacg cgaaaacctt
     4741 cctcaggacc ctggtccgag gtgtccctga gtatggctgc gtggtgaact tgcggaagac
     4801 agtggtgaac ttccctgtag aagacgaggc cctgggtggc acggcttttg ttcagatgcc
     4861 ggcccacggc ctattcccct ggtgcggcct gctgctggat acccggaccc tggaggtgca
     4921 gagcgactac tccagctatg cccggacctc catcagagcc agtctcacct tcaaccgcgg
     4981 cttcaaggct gggaggaaca tgcgtcgcaa actctttggg gtcttgcggc tgaagtgtca
     5041 cagcctgttt ctggatttgc aggtgaacag cctccagacg gtgtgcacca acatctacaa
     5101 gatcctcctg ctgcaggcgt acaggtttca cgcatgtgtg ctgcagctcc catttcatca
     5161 gcaagtttgg aagaacccca catttttcct gcgcgtcatc tctgacacgg cctccctctg
     5221 ctactccatc ctgaaagcca agaacgcagg gatgtcgctg ggggccaagg gcgccgccgg
     5281 ccctctgccc tccgaggccg tgcagtggct gtgccaccaa gcattcctgc tcaagctgac
     5341 tcgacaccgt gtcacctacg tgccactcct ggggtcactc aggacagccc agacgcagct
     5401 gagtcggaag ctcccgggga cgacgctgac tgccctggag gccgcagcca acccggcact
     5461 gccctcagac ttcaagacca tcctggactg agcggccgcc ctgcaggccc ctctccctcc
     5521 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5581 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5641 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5701 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5761 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     5821 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     5881 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     5941 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtacac atgctttaca
     6001 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     6061 ctttgaaaaa cacgatgata atatggccac attaattaat gggatcggcc attgaacaag
     6121 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     6181 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     6241 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     6301 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     6361 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     6421 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     6481 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     6541 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     6601 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     6661 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     6721 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     6781 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     6841 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     6901 gaatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     6961 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     7021 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     7081 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     7141 ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     7201 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     7261 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
     7321 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
     7381 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     7441 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
     7501 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
     7561 actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt
     7621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9061 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9121 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9181 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9241 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9301 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9361 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9421 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9481 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9541 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9601 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9721 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     9781 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     9841 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     9901 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     9961 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    10021 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
    10081 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
    10141 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
    10201 gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
    10261 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
    10321 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
    10381 ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag
    10441 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
    10501 gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaa
//
