LOCUS       sequence-333640-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAR-AD1.A6 part (C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pC-03
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(282..299)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(392..980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(472..491)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1151..1942,1943..2011))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1774..1793
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2012..2116)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2417..2439
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2431..2448
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2432..2448
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2459..2478
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2459..2477
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcaagg ttgaactgta tacattcccc gctgctccaa catttataca actaattaaa
       61 acaattcact gtaaaaactt ttcctagacg ctttgagacc agtaaaggag aggagctgtt
      121 tacaggcgtt gtacccatct tggttgagtt ggtacctgga gcacaagact ggcctcatgg
      181 gccttccgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaacat
      241 ggtcatagct gtttccttgc gtattgggcg ctctccgctt cctcgctcac tgactcgctg
      301 cgctcggtcg ttcgggtaaa gcctggggtg cctaatgagc aaaaggccag caaaaggcca
      361 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
      421 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
      481 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
      541 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
      601 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
      661 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
      721 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
      781 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
      841 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
      901 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
      961 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     1021 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     1081 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     1141 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     1201 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     1261 catctggccc cagtgctgca atgataccgc gagaaccacg ctcaccggct ccagatttat
     1321 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     1381 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     1441 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     1501 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     1561 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     1621 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     1681 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     1741 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     1801 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     1861 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     1921 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     1981 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     2041 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     2101 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     2161 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     2221 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtggccgcta
     2281 cagggcgctc ccattcgcca ttcaggctgc gcaactgttg ggaagggcgt ttcggtgcgg
     2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgacgta
     2461 atacgactca ctatagggcg aattggcgga aggccgtcaa ggccacgtgt cttgtccaga
     2521 gctc
//
