LOCUS       sequence-333641-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAR-DC part (C).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pC-04
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     174..196
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     188..205
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     189..205
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     216..235
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        216..234
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(563..580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(673..1261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(753..772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1432..2223,2224..2292))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2055..2074
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2293..2397)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caaaagaata gaccgagata gggttgagtg gccgctacag ggcgctccca ttcgccattc
       61 aggctgcgca actgttggga agggcgtttc ggtgcgggcc tcttcgctat tacgccagct
      121 ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
      181 acgacgttgt aaaacgacgg ccagtgagcg cgacgtaata cgactcacta tagggcgaat
      241 tggcggaagg ccgtcaaggc cacgtgtctt gtccagagct cggtctcaag gtaacaaaat
      301 aaagcaataa ggaatcgctc acccaaagga tctgctttga gaccagtaaa ggagaggagc
      361 tgtttacagg cgttgtaccc atcttggttg agttggatgg ggacgttaat ggtcataagt
      421 tttcggttag cggtacctgg agcacaagac tggcctcatg ggccttccgc tcactgcccg
      481 ctttccagtc gggaaacctg tcgtgccagc tgcattaaca tggtcatagc tgtttccttg
      541 cgtattgggc gctctccgct tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa
      601 agcctggggt gcctaatgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1081 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1561 aatgataccg cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc
     1621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     1681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2401 atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta
     2461 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     2521 aaat
//
