LOCUS       sequence-333647-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Stop codon part (E).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pE-01
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      611..1199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1100..1119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1292..1309
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1637..1656)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1638..1656)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1667..1684)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1667..1683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1676..1698)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1999..2103
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2104..2172,2173..2524,1..440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2322..2341)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
       61 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
      121 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
      181 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
      241 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggttctc
      301 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
      361 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
      421 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
      481 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
      541 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
      601 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      661 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
      721 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
      781 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
      841 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
      901 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
      961 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1021 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1081 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1141 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1201 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcattagg
     1261 caccccaggc tttacccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggagag
     1321 cgcccaatac gcaaggaaac agctatgacc atgttaatgc agctggcacg acaggtttcc
     1381 cgactggaaa gcgggcagtg agcggaaggc ccatgaggcc agtcttgtgc tccaggtacc
     1441 cctcgccgct aaccgaaaac ttatgaccat taacgtcccc atccaactca accaagatgg
     1501 gtacaacgcc tgtaaacagc tcctctcctt tactggtctc ggcggctaaa cgattcctag
     1561 accgacgtac gatacaacta gcgtgagacc gagctctgga caagacacgt ggccttgacg
     1621 gccttccgcc aattcgccct atagtgagtc gtattacgtc gcgctcactg gccgtcgttt
     1681 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1741 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgaaacgccc ttcccaacag
     1801 ttgcgcagcc tgaatggcga atgggagcgc cctgtagcgg ccactcaacc ctatctcggt
     1861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     1981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     2041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     2101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     2161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     2221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     2281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     2341 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     2401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     2461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     2521 atcg
//
