LOCUS       sequence-333651-        2961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP coding region part (F).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pF-01
FEATURES             Location/Qualifiers
     source          1..2961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..70)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(53..69)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(62..84)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        183..287
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(288..356,357..1148)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(506..525)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     protein_bind    1248..1272
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     rep_origin      1389..1977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1878..1897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2130..2152
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2149..2165
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2149..2165
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aggtcaggcg gaatggcact tccttattcc agatgcgtgc ggattatgca tgactggccg
       61 tcgttttaca acgtcgtgac tgggaaaacc ctggcgcttg tggtggatca cagttattgg
      121 agacttcctt ggccgtacga tgggttttgt ttcaggtatc tggcactttt cggggaaatg
      181 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      241 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      301 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      361 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      421 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      481 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      541 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      601 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      661 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      721 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      781 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
      841 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
      901 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
      961 ctggctggtt tattgctgat aaatctggag ccggtgagcg tggctctcgc ggtatcattg
     1021 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1081 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1141 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1201 tttaatttaa aaggatctag gtgaagatcc ttgaattgcc attatcaaca agtttgtaca
     1261 aaaaagcagg ctccgaattg gtatcacgag gacgatttga agccccctac ccatttacgc
     1321 tgacttgacg ggacggcggc tttgttgaat agagcgtcag accccgtaga aaagatcaaa
     1381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1441 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1501 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2041 tttccagcga catatgccat acgccgaact gaactccacc taatgtcttg gagattgtcg
     2101 gatagcgcgg aataggattg tgccctctga gcggataaca atttcacaca ggaaacagct
     2161 atgaccatga cacatgcatc caccatcgca gacttatcat cactgagcct ccacctagcc
     2221 tggtctcaga tggtgagtaa aggagaggag ctgtttacag gcgttgtacc catcttggtt
     2281 gagttggatg gggacgttaa tggtcataag ttttcggtta gcggcgaggg cgaaggcgac
     2341 gctacttacg gcaaattgac gcttaagttc atttgtacta caggaaaact tccagtcccc
     2401 tggccaactc tggttacaac acttacatac ggggtccaat gtttctcgcg ttacccagat
     2461 cacatgaagc agcacgactt ctttaaatct gcaatgccag aaggctatgt gcaagagcgc
     2521 acaatcttct tcaaggatga cgggaactat aagacacgcg ccgaggtcaa gtttgagggg
     2581 gatacgttag tgaatcgcat tgaattgaaa ggcattgact ttaaagaaga tggcaacatt
     2641 ttgggacata aactggagta taactataat tcgcacaacg tgtatatcat ggctgacaag
     2701 cagaagaacg ggattaaggt aaatttcaag attcgtcaca acattgagga tggcagcgtg
     2761 caattggctg accattatca acaaaacaca cccattggcg acggcccggt tttacttcct
     2821 gacaatcact acctgagtac acaatccaaa ttatcgaagg acccaaatga gaaacgtgat
     2881 cacatggtcc ttttggagtt cgtaaccgcg gcggggatta ctttaggcat ggatgagttg
     2941 tataagtgag gcggcgagac c
//
