LOCUS       sequence-333653-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target AD1.A5 part (G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pG-02
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      420..1008
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     909..928
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1101..1118
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(1446..1465)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1447..1465)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1476..1493)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1476..1492)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1485..1507)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        1808..1912
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1913..1981,1982..2524,1..249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2131..2150)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
       61 gctggctggt ttattgctga taaatctgga gccggtgagc gtggttctcg cggtatcatt
      121 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
      181 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
      241 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      301 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      361 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      421 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      481 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      541 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      601 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      661 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      721 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
      781 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
      841 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
      901 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
      961 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1021 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcattaggc accccaggct
     1081 ttacccgaac gaccgagcgc agcgagtcag tgagcgagga agcggagagc gcccaatacg
     1141 caaggaaaca gctatgacca tgttaatgca gctggcacga caggtttccc gactggaaag
     1201 cgggcagtga gcggaaggcc catgaggcca gtcttgtgct ccaggtaccc tcctctcctt
     1261 tactggtctc aagtaagttt ttacagtgaa ttgttttaat tagttgtata aatgttggag
     1321 cagcggggaa tgtatacagt tcatgtatat attccccgct ttttttttgg atctaggagg
     1381 aaggatctga tgtgagaccg agctctggac aagacacgtg gccttgacgg ccttccgcca
     1441 attcgcccta tagtgagtcg tattacgtcg cgctcactgg ccgtcgtttt acaacgtcgt
     1501 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     1561 agctggcgta atagcgaaga ggcccgcacc gaaacgccct tcccaacagt tgcgcagcct
     1621 gaatggcgaa tgggagcgcc ctgtagcggc cactcaaccc tatctcggtc tattcttttg
     1681 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1741 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1801 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1861 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     1921 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     1981 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2041 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2101 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2161 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2221 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     2281 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     2341 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     2401 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     2461 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     2521 acaa
//
