LOCUS       sequence-333655-        2524 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Target DC (G).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Greco FV, Pandi A, Erb TJ, Grierson CS, Gorochowski TE
  TITLE     Harnessing the central dogma for stringent multi-level control of 
            gene expression.
  JOURNAL   Nat Commun. 2021 Mar 19;12(1):1738. doi: 10.1038/s41467-021-21995-7.
  PUBMED    33741937
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-19"; volume: "12(1):1738. doi"; pages: " 
            10.1038/s41467-021-21995-7"
COMMENT     Sequence Label: pG-04
FEATURES             Location/Qualifiers
     source          1..2524
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(303..320)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(413..1001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(493..512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1172..1963,1964..2032))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1795..1814
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2033..2137)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2438..2460
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2452..2469
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2453..2469
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2480..2499
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2480..2498
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cacgtgtctt gtccagagct cggtctcaca tcaaaaaatc gactccttaa tctcaatttc
       61 gtttaaggaa tcgctcaccc atacttgaga ccagtaaagg agaggagctg tttacaggcg
      121 ttgtacccat cttggttgag ttggatgggg acgttaatgg tcataagttt tggtacctgg
      181 agcacaagac tggcctcatg ggccttccgc tcactgcccg ctttccagtc gggaaacctg
      241 tcgtgccagc tgcattaaca tggtcatagc tgtttccttg cgtattgggc gctctccgct
      301 tcctcgctca ctgactcgct gcgctcggtc gttcgggtaa agcctggggt gcctaatgag
      361 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      421 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      481 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      541 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      601 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      661 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      721 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      781 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      841 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagta accttcggaa
      901 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      961 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1021 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1081 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1141 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1201 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1261 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagaaccac
     1321 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1381 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1441 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1501 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1561 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1621 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1681 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1741 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1801 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1861 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1921 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1981 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2041 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2101 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2161 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     2221 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     2281 gatagggttg agtggccgct acagggcgct cccattcgcc attcaggctg cgcaactgtt
     2341 gggaagggcg tttcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     2401 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     2461 acggccagtg agcgcgacgt aatacgactc actatagggc gaattggcgg aaggccgtca
     2521 aggc
//
