LOCUS       sequence-333657-       13625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing the coding sequence of the
            Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding 
            site mutated under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13625)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 13625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_EVDm1
FEATURES             Location/Qualifiers
     source          1..13625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        612..956
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     900..920
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1057..1081
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5300..5324)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      5337..5346
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        5744..5923
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             5965..6516
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      6536..6788
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6881..6905
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7426..8217
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8463..9051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8952..8971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9205..9222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9237..9377
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9303..9322)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9463..9485
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9721..9915
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9981..11054)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11483..12112)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13443..13467
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    13536..13556
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        13559..13577
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     13559..13576
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 actagagcca agctgatctc ctttgccccg gagatcacca tggacgactt tctctatctc
       61 tacgatctag gaagaaagtt cgacggagaa ggtgacgata ccatgttcac caccgataat
      121 gagaagatta gcctcttcaa tttcagaaag aatgctgacc cacagatggt tagagaggcc
      181 tacgcggcag gtctcatcaa gacgatctac ccgagtaata atctccagga gatcaaatac
      241 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
      301 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
      361 cttcataaac caaggcaagt aatagagatt ggagtctcta agaaagtagt tcctactgaa
      421 tcaaaggcca tggagtcaaa aattcagatc gaggatctaa cagaactcgc cgtgaagact
      481 ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat cttcgtcaac
      541 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
      601 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
      661 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
      721 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cccctgccga cagtggtccc
      781 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
      841 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
      901 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggactccg
      961 gtatttttac aacaattacc acaacaaaac aaacaacaaa caacattaca atttactatt
     1021 ctagtcgacc tgcaggcggc cgcactagtg atatcagcaa gtttgtacaa aaaagcaggc
     1081 ttcatggaga cttctcaaaa gatgattact acggtgatac ttcaaggggg aaactaccta
     1141 acttggtcaa gaacaacaaa aacggtgttg tgtggtcgtg ggctttggag ccatgtaatc
     1201 tcatctaaag ctccaaaaga agccaaagaa gaagaagaaa cggagactat atctcccgag
     1261 gaagaaaaat ggttccaaga ggatcaagct gtccttgctc ttcttcaaaa ctctcttgaa
     1321 acctctattc tagagggata ctcttactgt gagacagcaa aagagttgtg ggatacactt
     1381 aaaaacgtgt atgggaacga atcgaatttg acccgcgtgt ttgaagtgaa gaaggcgatt
     1441 aacgaactaa gtcaagaaga cttagagttt actaaacact ttggtaagtt caggtcctta
     1501 tggtctgaac ttgagagctt gaggccggga acgcttgacc cgaagattct ccacgaaagg
     1561 cgtgagcaag acaaggtgtt tggactcctt ctcaccttaa atccagggta caatgaccta
     1621 attaaacact tgcttaggtc ggagaagtta ccaagtcttg atgaggtgtg ttctaagatt
     1681 cagaaagagc aaggatcaac cgggctattt ggtggaaaaa gtgaactcat cacggctaac
     1741 aaaggagaag tagtggcgaa caaaggtgtg tacaagaatg aggatagaaa actgttgact
     1801 tgtgatcatt gtaagaagaa gggccacacc aaagacaaat gttggttgct acatccacac
     1861 ctcaagcccg ccaagttcaa agactcaaga gctcatctct ctcaagaaac tcatgaggag
     1921 caaagtcaag ccgggtcatc gaaaggagag acatccacat cattcggaga ctatgtgagg
     1981 aagtcggact tggaagcact catcaagtcc attgcttcac tcaaagaatc caacctcact
     2041 ttctcatcac aaacatctag tagttcaatt gtgatagact cgggtgcttc tcatcatatg
     2101 attagtaact ctaatcttct tgataacata gaaccagctc taggacatgt tattattgct
     2161 aatggtgaca aagttccaat tgaagggata ggaaacttga aattgtttaa caaagattca
     2221 aaagctttct ttatgcctaa gttcacatct aacttattat ccgtgaaaag gaccactaga
     2281 gacttaaatt gttatgcgat ttttggccct aatgatgtgt attttcagga tattgagacg
     2341 ggaaaagtta ttggagaagg tggttcaaaa ggagaactct atgttcttga agacttgtca
     2401 ccaaactctt ctagctgttt ttcctctaag tcacatttag gcatctcttt taatactttg
     2461 tggcatgcta ggctaggtca tcctcatact agagctctta aactcatgtt accaaatatt
     2521 tcttttgatc atacaagttg tgaggcttgt attcttggta aacattgcaa atcggttttt
     2581 cctaaatctc ttactatcta tgagaaatgt tttgatctag ttcattcgga tgtttggacc
     2641 tcaccatgtg tttctagaga caacaacaaa tattttgtca catttattga tgagaaatca
     2701 aagtacactt ggataacact attaccctca aaagatagag tgtttgaagc tttcactaac
     2761 tttgaaacct atgtcactaa tcaatttaat gcaaagatta aagtgtttag gaccgataat
     2821 ggaggagaat acacaagcca aaagtttcga gatcatctag ccaaacgtgg aataatacac
     2881 caaacaagtt gcccgtacac accacaacaa aatggagtgg cggagagaaa gaaccgacat
     2941 ctcatggagg tggcgcggtc aatgatgttt catacaagtg ttccaaagag attttggggt
     3001 gatgcggtct taaccgcgtg ctaccttatc aatcgaacac caactaaagt cttaagtgac
     3061 ttatctccct tcgaggtact taacaatatt aaaccgttca ttgatcactt acgagtgttt
     3121 ggttgtgtat gttttgtttt gatacccgga gaacaaagaa gcaagctaga tgcgaagagc
     3181 actaagtgta tgtttcttgg ctactcaact actcaaaaag gatataaatg ctttgatcca
     3241 accaagaacc gcacatttat atcccgagat gtcaagtttc ttgaaaacca agactacaac
     3301 aacaagaagg attgggagaa cctaaaagat cttactcatt ccacctccga tcgtgttgca
     3361 actctaaagt ttctattgga tcatttgggc aatgactcaa cttcgacaac tcaacatcaa
     3421 ccggagatga cacaagatca agaagacttg aatcaagaga atgaggaggt gtccctacaa
     3481 catcaagaaa atcttacaca cgtgcaagaa gatcctccaa acacacaaga acactctgaa
     3541 catgttcaag aaatccaaga tgactcaagt gaagatgaag aacgtacaca agttcttcca
     3601 cctccaccac ctcttagaag gagtacacgc attcgacgca agaaggaatt cttcaactct
     3661 aatgcggtag ctcatccttt ccaagcgaca tgttctcttg cccttgtacc tctagatcat
     3721 caagcgtttc tctccaagat ttctgagcat cggattcctc aaacatatga agaggcaatg
     3781 gaagtcaagg aatggaggga tgcgatagcc gatgagataa acgctatgaa acgaaatcat
     3841 acttgggatg aagatgactt acctaaagga aagaaaacag tgagctcaag gtgggtattc
     3901 acaatcaagt ataagagtaa tggagatatc gagagatata aaactcgctt ggtggctcgg
     3961 gggttcacgc aaacatatgg ctccgactac atggagactt ttgctccggt ggctaagcta
     4021 cacacggtga gagtggttct tgccttagca acaaatttat catggggact atggcaaatg
     4081 gatgtcaaga atgcatttct ccaaggcgaa cttgaagatg atgtgtatat gacgcctcca
     4141 cccggtctag aagataccat tccatgtgat aaagtgttga gactacgcaa agctatctat
     4201 ggtcttaaac aatctccacg tgcatggtac cacaagctca gccgaactct taaagatcat
     4261 ggattcaaga agtccgagtc cgatcacact ttgtttacat tacaaagtcc tcaaggtatt
     4321 gtcgttgttc tcatctatgt tgatgatctt atcatcaccg gagacaacaa agacggtata
     4381 gactctacca agacttttct taaatcttgt tttgatatta aagatcttgg tgaactcaaa
     4441 tacttcctag gaattgaagt gtgtcgctct aatgctggac tctttctatc acaacgcaaa
     4501 tacactcttg acttgttgaa tgagacaggt tttatggacg ctaaaccggc tcgcactcca
     4561 cttgaagatg gctacaaggt caatcgaaag ggggagaaag aagatgagaa gtttggcgat
     4621 gcacctctct atcgcaaact tgtaggtaag ttgatctatt tgacaaacac aagacctgac
     4681 atttgttttg cggtaaatca ggttagtcaa cacatgaaag tcccgatggt gtatcattgg
     4741 aacatggtgg agagaatact aaggtatctc aaaggcagct ccggacaagg catatggatg
     4801 ggaaagaact caagcaccga gatagtggga tattgcgatg cggactatgc gggtgataga
     4861 ggagatagaa gatctacaac tggttattgt acgttcattg gaggtaacct tgcaacttgg
     4921 aagacaaaga aacagaaggt ggtctcatgt tcgagtgcag aatcggagta tagagcaatg
     4981 agaaagctca ccaacgaact cacttggctt aaagctcttc ttaaagatgt tggcattgaa
     5041 caacacatgc ctatcaccat gcattgtgac aacaaggcag caatatacat agcctcaaat
     5101 tcggtgtttc atgagagaac caagcatatt gaagtcgatt gtcataaagt ccgagagaag
     5161 atcatcgaag gagtcacact accgtgttat acaagaagtg aagatcagtt ggcggatatc
     5221 ttcaccaaag ccgcaagtct caaagtttgc aacttcatac atggcaaatt aggacttgtg
     5281 gatctatctc actcttgaaa cccagctttc ttgtacaaag tggtgatatc ccgcggccat
     5341 ggcggccggg agcatgcgac gtcgatctaa ctgactagcc gcggccatgc tagagtccgc
     5401 aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca gaataatgtg
     5461 tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat gtgttgagca
     5521 tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta
     5581 attcctaaaa ccaaaatcca gtgacctgca ggcatgcgac gtcgggccct ctagaggatc
     5641 cccgggtacc gcgaattatc gatcatgagc ggagaattaa gggagtcacg ttatgacccc
     5701 cgccgatgac gcgggacaag ccgttttacg tttggaactg acagaaccgc aacgttgaag
     5761 gagccactga gccgcgggtt tctggagttt aatgagctaa gcacatacgt cagaaaccat
     5821 tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta gcaaatattt cttgtcaaaa
     5881 atgctccact gacgttccat aaattcccct cggtatccaa ttagagtctc atattcactc
     5941 tcaactcgat cgaggggatc taccatgagc ccagaacgac gcccggccga catccgccgt
     6001 gccaccgagg cggacatgcc ggcggtctgc accatcgtca accactacat cgagacaagc
     6061 acggtcaact tccgtaccga gccgcaggaa ccgcaggagt ggacggacga cctcgtccgt
     6121 ctgcgggagc gctatccctg gctcgtcgcc gaggtggacg gcgaggtcgc cggcatcgcc
     6181 tacgcgggtc cctggaaggc acgcaacgcc tacgactgga cggccgagtc gaccgtgtac
     6241 gtctcccccc gccaccagcg gacgggactg ggctccacgc tctacaccca cctgctgaag
     6301 tccctggagg cacagggctt caagagcgtg gtcgctgtca tcgggctgcc caacgacccg
     6361 agcgtgcgca tgcacgaggc gctcggatat gccccccgcg gcatgctgcg ggcggccggc
     6421 ttcaagcacg ggaactggca tgacgtgggt ttctggcagc tggacttcag cctgccggtg
     6481 ccgccccgtc cggtcctgcc cgtcaccgaa atctgatgac ccctagagtc aagcagatcg
     6541 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     6601 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     6661 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     6721 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     6781 actagatcga ccggcatgca agctgataat tcaattcggc gttaattcag tacattaaaa
     6841 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
     6901 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
     6961 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
     7021 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
     7081 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
     7141 tcaattcggg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag
     7201 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc
     7261 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg
     7321 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     7381 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     7441 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     7501 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     7561 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     7621 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     7681 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     7741 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     7801 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     7861 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     7921 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     7981 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     8041 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     8101 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     8161 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     8221 ctagctagaa attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     8281 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     8341 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc
     8401 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     8461 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     8521 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     8581 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     8641 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     8701 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     8761 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     8821 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     8881 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     8941 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     9001 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     9061 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     9121 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     9181 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     9241 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     9301 agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg
     9361 actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     9421 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     9481 agaggttttc accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg
     9541 cggtcgagtg gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca
     9601 gccatttttg agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag
     9661 cgcagcgacc gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt
     9721 tatgcacagg ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa
     9781 aaatcgcctt ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac
     9841 ggctttgggt tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg
     9901 tacgtgctat ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc
     9961 cctagcatct gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg
    10021 tagcgcatga ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc
    10081 aggtcatttg acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg
    10141 ctgccactgc gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg
    10201 cggcgtgcca ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg
    10261 tgaaccgtca gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct
    10321 agctcgatct cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta
    10381 atcaaggctt caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc
    10441 tgcatggcaa cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc
    10501 tttccgccat cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg
    10561 cggccgggct tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa
    10621 accgccatca gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa
    10681 acctctacgt gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc
    10741 ttcgacagac ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca
    10801 tagagcatcg gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac
    10861 ggcgcaccgg ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc
    10921 cacgattcac cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc
    10981 ttcaacttct ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt
    11041 ttgcgaccgc tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc
    11101 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca
    11161 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc
    11221 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc
    11281 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc
    11341 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt
    11401 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc
    11461 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct
    11521 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg
    11581 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc
    11641 cggccagcgc ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga
    11701 caaaggccgc ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca
    11761 ggtcggcggt ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg
    11821 ctgccttgat cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga
    11881 agtcgcgccg gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa
    11941 cggttagcgg ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg
    12001 aatcgactaa cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga
    12061 tggtcgtctt gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc
    12121 cttgcgtatt tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct
    12181 gttttactca aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa
    12241 gctggccggc caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg
    12301 cacggttgag acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc
    12361 gatctcttcg gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt
    12421 tcctcttggc gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca
    12481 cggaaggcac cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg
    12541 cggtacaggg tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct
    12601 tcctggtcga tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg
    12661 ccaaacttca cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt
    12721 agggaacgct cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc
    12781 gtggtggtgt cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg
    12841 cgctcggcaa tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact
    12901 gtcacaacgt cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc
    12961 ctggtgccgg tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc
    13021 cgttggttgg tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg
    13081 gcggcgctct tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg
    13141 cgactaaaac acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta
    13201 acttaggact tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg
    13261 actgcactat agcagcggag gggttggatc aaagtacttt aaagtacttt aaagtacttt
    13321 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    13381 gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt
    13441 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg
    13501 acaatctgat ccaagctcaa gctaagcttg agctccaact ttgtatagaa aagttgtcat
    13561 ttaggtgaca ctatagaata ctcaagctat gcatccaacg cgttgggagc tctcccatat
    13621 ggtcg
//
