LOCUS       sequence-333670-        1359 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vitro transcription for rescue experiments.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Lee YR, Kim SH, Ben-Mahmoud A, Kim OH, Choi TI, Lee KH, Ku B, Eum J,
            Kee Y, Lee S, Cha J, Won D, Lee ST, Choi JR, Lee JS, Kim HD, Kim HG,
            Bonkowsky JL, Kang HC, Kim CH
  TITLE     Eif2b3 mutants recapitulate phenotypes of vanishing white matter 
            disease and validate novel disease alleles in zebrafish.
  JOURNAL   Hum Mol Genet. 2021 Apr 27;30(5):331-342. doi: 10.1093/hmg/ddab033.
  PUBMED    33517449
REFERENCE   2  (bases 1 to 1359)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Hum Mol Genet"; date: "2021-04-27- 27"; volume: "30"; issue: "5"; 
            pages: "331-342"
COMMENT     Sequence Label: EIF2B3-R225Q
FEATURES             Location/Qualifiers
     source          1..1359
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             251..262
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggaatttc aagcagtagt gatggcagta ggtggaggat ctcggatgac agacctaact
       61 tccagcattc ccaaacctct gcttccagtt gggaacaaac ctttaatttg gtacccattg
      121 aacctgcttg agcgtgttgg atttgaagaa gtcattgtgg ttacaaccag ggatgttcaa
      181 aaggctctat gtgcagaatt caagatgaaa atgaagccag atattgtgtg tattcctgat
      241 gatgctgaca tgggaactgc agattctttg cgctacatat atccaaaact taagacagat
      301 gtgctggtgc tgagctgtga tctgataaca gacgttgcct tacatgaggt tgtggacctg
      361 tttagagctt atgatgcatc acttgctatg ttgatgagaa aaggccaaga tagcatagaa
      421 cctgttcccg gtcaaaaggg gaaaaaaaaa gcagtggagc agcgtgactt cattggagtg
      481 gacagcacag gaaagaggct gctcttcatg gctaatgaag cagacttgga tgaagagctg
      541 gtcattaagg gatccatcct acagaagcat cctagaatac gtttccacac gggtcttgtg
      601 gatgcccacc tctactgttt gaaaaaatac atcgtggact tcctaatgga aaatgggtca
      661 ataacttcta tccagagtga actgattcca tatttagtga gaaaacagtt ttcctcagct
      721 tcctcacaac agggacaaga agaaaaagag gaggatctaa agaaaaagga gctgaagtcc
      781 ttagatatct acagttttat aaaagaagcc aatacactga acctggctcc ctatgatgcc
      841 tgctggaatg cctgtcgagg agacaggtgg gaagacttgt ccagatcaca ggtgcgctgc
      901 tatgtccaca tcatgaaaga ggggctctgc tctcgagtga gcacactggg actctacatg
      961 gaagcaaaca gacaggtgcc caaattgctg tctgctctct gtccagaaga accaccagtc
     1021 cattcgtcag cccagattgt cagcaaacac ctggttggag ttgacagcct cattgggcca
     1081 gagacacaga ttggagagaa gtcatccatt aagcgctcag tcattggctc atcctgtctc
     1141 ataaaagata gagtgactat taccaattgc cttctcatga actcagtcac tgtggaggaa
     1201 ggaagcaata tccaaggcag tgtcatctgc aacaatgctg tgatcgagaa gggtgcagac
     1261 atcaaggact gcttgattgg aagtggccag aggattgaag ccaaagctaa acgagtgaat
     1321 gaggtgatcg tggggaatga ccagctcatg gagatctga
//
