LOCUS       sequence-333679-       11969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plant binary expression vector containing mGFP5-GUS fusion reporter 
            under CaMV 35S promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11969)
  AUTHORS   Oberlin S, Sarazin A, Chevalier C, Voinnet O, Mari-Ordonez A
  TITLE     A genome-wide transcriptome and translatome analysis of Arabidopsis 
            transposons identifies a unique and conserved genome expression 
            strategy for Ty1/Copia retroelements.
  JOURNAL   Genome Res. 2017 Sep;27(9):1549-1562. doi: 10.1101/gr.220723.117. 
            Epub 2017 Aug 7.
  PUBMED    28784835
REFERENCE   2  (bases 1 to 11969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.220723.117"; journalName: "Genome Res"; date: "2017-09";
            volume: "27"; issue: "9"; pages: "1549-1562"
COMMENT     Sequence Label: p35S_GFP-GUS
FEATURES             Location/Qualifiers
     source          1..11969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1..18
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        679..1023
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     967..987
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1124..1148
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(1179..1207)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1802..1822
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             1899..3710
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     protein_bind    complement(3711..3735)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3748..3757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        4155..4334
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4376..4927
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     terminator      4947..5199
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    5292..5316
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5837..6628
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6874..7462
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7363..7382
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7616..7633
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7648..7788
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7714..7733)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7874..7896
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8132..8326
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8392..9465)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9894..10523)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11854..11878
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11947..11967
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 atttaggtga cactatagaa tactcaagct atgcatccaa cgcgttggga gctctcccat
       61 atggtcgact agagccaagc tgatctcctt tgccccggag atcaccatgg acgactttct
      121 ctatctctac gatctaggaa gaaagttcga cggagaaggt gacgatacca tgttcaccac
      181 cgataatgag aagattagcc tcttcaattt cagaaagaat gctgacccac agatggttag
      241 agaggcctac gcggcaggtc tcatcaagac gatctacccg agtaataatc tccaggagat
      301 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa
      361 gaacacagag aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca
      421 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaga aagtagttcc
      481 tactgaatca aaggccatgg agtcaaaaat tcagatcgag gatctaacag aactcgccgt
      541 gaagactggc gaacagttca tacagagtct tttacgactc aatgacaaga agaaaatctt
      601 cgtcaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
      661 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
      721 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
      781 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgccc ctgccgacag
      841 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
      901 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
      961 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
     1021 gactccggta tttttacaac aattaccaca acaaaacaaa caacaaacaa cattacaatt
     1081 tactattcta gtcgacctgc aggcggccgc actagtgata tcagcaagtt tgtacaaaaa
     1141 agcaggctat gagtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
     1201 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
     1261 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
     1321 caaccctggt caccaccctg acctacggcg tgcagtgctt ctcccgttac cctgatcata
     1381 tgaagcggca cgacttcttc aagagcgcca tgcctgaggg atacgtgcag gagaggacca
     1441 tcttcttcaa ggacgacggg aactacaaga cacgtgctga agtcaagttt gagggagaca
     1501 ccctcgtcaa caggatcgag cttaagggaa tcgatttcaa ggaggacgga aacatcctcg
     1561 gccacaagtt ggaatacaac tacaactccc acaacgtata catcatggcc gacaagcaaa
     1621 agaacggcat caaagccaac ttcaagaccc gccacaacat cgaagacggc ggcgtgcaac
     1681 tcgctgatca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1741 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca
     1801 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggta gaactataca
     1861 aactcgaggc agctgcagct gcagctgcag ctcctaggat gttacgtcct gtagaaaccc
     1921 caacccgtga aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact
     1981 gtggaattga tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc
     2041 caggcagttt taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct
     2101 ggtatcagcg cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt
     2161 tcgatgcggt cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc
     2221 agggcggcta tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg
     2281 tacgtatcac cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg
     2341 tgattaccga cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg
     2401 ccggaatcca tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca
     2461 ccgtggtgac gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg
     2521 ccaatggtga tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac
     2581 aaggcactag cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt
     2641 atctctatga actgtgcgtc acagccaaaa gccagacaga gtgtgatatc tacccgcttc
     2701 gcgtcggcat ccggtcagtg gcagtgaagg gcgaacagtt cctgattaac cacaaaccgt
     2761 tctactttac tggctttggt cgtcatgaag atgcggactt gcgtggcaaa ggattcgata
     2821 acgtgctgat ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta
     2881 cctcgcatta cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg
     2941 tgattgatga aactgctgct gtcggcttta acctctcttt aggcattggt ttcgaagcgg
     3001 gcaacaagcc gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc
     3061 acttacaggc gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt
     3121 ggagtattgc caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac
     3181 tggcggaagc aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt
     3241 tctgcgacgc tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt
     3301 attacggatg gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag
     3361 aacttctggc ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg
     3421 atacgttagc cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg
     3481 catggctgga tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg
     3541 tatggaattt cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga
     3601 aagggatctt cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct
     3661 ggactggcat gaacttcggt gaaaaaccgc agcagggagg caaacaatga acccagcttt
     3721 cttgtacaaa gtggtgatat cccgcggcca tggcggccgg gagcatgcga cgtcgatcta
     3781 actgactagc cgcggccatg ctagagtccg caaaaatcac cagtctctct ctacaaatct
     3841 atctctctct atttttctcc agaataatgt gtgagtagtt cccagataag ggaattaggg
     3901 ttcttatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     3961 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtgacctgc
     4021 aggcatgcga cgtcgggccc tctagaggat ccccgggtac cgcgaattat cgatcatgag
     4081 cggagaatta agggagtcac gttatgaccc ccgccgatga cgcgggacaa gccgttttac
     4141 gtttggaact gacagaaccg caacgttgaa ggagccactg agccgcgggt ttctggagtt
     4201 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
     4261 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
     4321 tcggtatcca attagagtct catattcact ctcaactcga tcgaggggat ctaccatgag
     4381 cccagaacga cgcccggccg acatccgccg tgccaccgag gcggacatgc cggcggtctg
     4441 caccatcgtc aaccactaca tcgagacaag cacggtcaac ttccgtaccg agccgcagga
     4501 accgcaggag tggacggacg acctcgtccg tctgcgggag cgctatccct ggctcgtcgc
     4561 cgaggtggac ggcgaggtcg ccggcatcgc ctacgcgggt ccctggaagg cacgcaacgc
     4621 ctacgactgg acggccgagt cgaccgtgta cgtctccccc cgccaccagc ggacgggact
     4681 gggctccacg ctctacaccc acctgctgaa gtccctggag gcacagggct tcaagagcgt
     4741 ggtcgctgtc atcgggctgc ccaacgaccc gagcgtgcgc atgcacgagg cgctcggata
     4801 tgccccccgc ggcatgctgc gggcggccgg cttcaagcac gggaactggc atgacgtggg
     4861 tttctggcag ctggacttca gcctgccggt gccgccccgt ccggtcctgc ccgtcaccga
     4921 aatctgatga cccctagagt caagcagatc gttcaaacat ttggcaataa agtttcttaa
     4981 gattgaatcc tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta
     5041 agcatgtaat aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta
     5101 gagtcccgca attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg
     5161 ataaattatc gcgcgcggtg tcatctatgt tactagatcg accggcatgc aagctgataa
     5221 ttcaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     5281 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     5341 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     5401 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     5461 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     5521 tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata
     5581 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
     5641 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
     5701 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
     5761 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
     5821 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     5881 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     5941 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     6001 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     6061 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     6121 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     6181 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     6241 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     6301 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     6361 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     6421 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     6481 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     6541 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     6601 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     6661 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     6721 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa
     6781 attgggagat atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6841 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6901 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6961 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7021 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7081 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7141 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7201 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7261 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7321 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7381 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7441 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7501 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7561 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     7621 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg
     7681 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7741 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     7801 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     7861 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     7921 gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc
     7981 gcgccctggt agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat
     8041 aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg
     8101 cagctcttcg gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt
     8161 tttaataagt tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt
     8221 cacttacatg tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg
     8281 gttcccaatg tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt
     8341 tcgacctttt tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc
     8401 ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg
     8461 ccccgcctcc tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac
     8521 ggtgaaacag aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa
     8581 caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc
     8641 gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc
     8701 ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc
     8761 ggtttcgctc tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac
     8821 caggcggccg ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg
     8881 aatgcaggtt tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt
     8941 gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg
     9001 gtatcggttc atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca
     9061 cacactggcc atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta
     9121 gcggatcacc tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat
     9181 gatgctgcga ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg
     9241 ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga
     9301 ttctttgcgg attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc
     9361 gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag
     9421 cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc
     9481 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     9541 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     9601 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     9661 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     9721 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     9781 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
     9841 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
     9901 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
     9961 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    10021 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    10081 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    10141 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    10201 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    10261 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    10321 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    10381 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    10441 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    10501 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10561 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    10621 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    10681 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    10741 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    10801 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    10861 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    10921 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    10981 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    11041 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    11101 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    11161 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    11221 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    11281 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    11341 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    11401 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    11461 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    11521 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    11581 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    11641 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    11701 caaagtactt taaagtactt taaagtactt taaagtactt tgatcccgag gggaaccctg
    11761 tggttggcat gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat
    11821 ccgattattc taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa
    11881 cactgatagt ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctaagctt
    11941 gagctccaac tttgtataga aaagttgtc
//
