LOCUS       sequence-333688-        5882 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express TonB H20A protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Larsen RA, Deckert GE, Kastead KA, Devanathan S, Keller KL, Postle K
  TITLE     His(20) provides the sole functionally significant side chain in the
            essential TonB transmembrane domain.
  JOURNAL   J Bacteriol. 2007 Apr;189(7):2825-33. doi: 10.1128/JB.01925-06. Epub
            2007 Feb 2.
  PUBMED    17277053
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/JB.01925-06"; journalName: "J Bacteriol"; date: "2007-04"; 
            volume: "189"; issue: "7"; pages: "2825-33"
COMMENT     Sequence Label: pKP381
FEATURES             Location/Qualifiers
     source          1..5882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     208..227
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     terminator      1065..1096
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(2925..3584)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3499..3518
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(3585..3687)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(4079..4096)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4213..4758)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(4293..4312)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4978..5856)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg
      301 ctagacctgg tttttctact gaaatgatta tgacttcaat gacccttgat ttacctcgcc
      361 gcttcccctg gccgacgtta ctttcggtct gcatcgctgg tgctgttgtg gcgggtctgc
      421 tctatacctc ggtacatcag gttattgaac tacctgcgcc tgcgcagccg atttctgtca
      481 cgatggttac gcctgctgat ctcgaaccgc cacaagccgt tcagccgcca ccggagccgg
      541 tggtagagcc agaaccggaa cctgagccga tccccgaacc gccaaaagaa gcaccggtgg
      601 tcattgaaaa gccgaagccg aaacctaagc caaaaccgaa gccggtgaaa aaggtacagg
      661 agcagccaaa acgcgatgtc aaacccgtag agtcgcgtcc ggcatcaccg tttgaaaata
      721 cggcaccggc acgcctgaca tcaagtacag caacggctgc aaccagcaag ccggttacca
      781 gtgtggcttc aggaccacgc gcattaagcc gtaatcagcc gcagtatccg gcacgagcac
      841 aggcattgcg cattgaaggg caggttaaag ttaaatttga cgttacgccg gatggtcgcg
      901 tggataacgt acaaatcctc tcagccaagc ctgcgaacat gtttgagcgt gaggtgaaaa
      961 atgcgatgcg cagatggcgt tatgagccgg gtaagccagg cagtgggatt gtggtgaata
     1021 tcctgtttaa aattaacggc accaccgaaa ttcagtaagc agaaagtcaa aagcctccga
     1081 ccggaggctt ttgactatta ctcaacaggt aaggcgcgag gttttccttc aggatcaacc
     1141 gcgacatact ggggatcctc tagagtcgac ctgcaggcat gcaccattcc ttgcggcggc
     1201 ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt cgcataaggg
     1261 agagcgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg
     1321 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
     1381 ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat
     1441 gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac
     1501 tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca tggcggccga
     1561 cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct tccccattat
     1621 gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc tgtccaggca
     1681 ggtagatgac gaccatcagg gacagcttca aggatcgctc gcggctctta ccagcctaac
     1741 ttcgatcatt ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa
     1801 cgggttggca tggattgtag gcgccgccct ataccttgtc tgcctccccg cgttgcgtcg
     1861 cggtgcatgg agccgggcca cctcgacctg aatggaagcc ggcggcacct cgctaacgga
     1921 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     1981 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
     2041 tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     2101 cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     2161 acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     2221 ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc ctacgtgctg ctgaagttgc
     2281 ccgcaacaga gagtggaacc aaccggtgat accacgatac tatgactgag agtcaacgcc
     2341 atgagcggcc tcatttctta ttctgagtta caacagtccg caccgctgcc ggtagctcct
     2401 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     2461 aactcgtagg acaggtgccg gcagcgccca acagtccccc ggccacgggg cctgccacca
     2521 tacccacgcc gaaacaagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct
     2581 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctaaccg tttttatcag
     2641 gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg ggagagcctg
     2701 agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg tagtcaataa
     2761 accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg aaccgacgac
     2821 cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact tattcaggcg
     2881 tagcaaccag gcgtttaagg gcaccaataa ctgccttaaa aaaattacgc cccgccctgc
     2941 cactcatcgc agtactgttg taattcatta agcattctgc cgacatggaa gccatcacaa
     3001 acggcatgat gaacctgaat cgccagcggc atcagcacct tgtcgccttg cgtataatat
     3061 ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa
     3121 ctggtgaaac tcacccaggg attggctgag acgaaaaaca tattctcaat aaacccttta
     3181 gggaaatagg ccaggttttc accgtaacac gccacatctt gcgaatatat gtgtagaaac
     3241 tgccggaaat cgtcgtggta ttcactccag agcgatgaaa acgtttcagt ttgctcatgg
     3301 aaaacggtgt aacaagggtg aacactatcc catatcacca gctcaccgtc tttcattgcc
     3361 atacggaatt ccggatgagc attcatcagg cgggcaagaa tgtgaataaa ggccggataa
     3421 aacttgtgct tatttttctt tacggtcttt aaaaaggccg taatatccag ctgaacggtc
     3481 tggttatagg tacattgagc aactgactga aatgcctcaa aatgttcttt acgatgccat
     3541 tgggatatat caacggtggt atatccagtg atttttttct ccattttagc ttccttagct
     3601 cctgaaaatc tcgataactc aaaaaatacg cccggtagtg atcttatttc attatggtga
     3661 aagttggaac ctcttacgtg ccgatcaacg tctcattttc gccaaaagtt ggcccagggc
     3721 ttcccggtat caacagggac accaggattt atttattctg cgaagtgatc ttccgtcaca
     3781 ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca acttactgat ttagtgtatg
     3841 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
     3901 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct agcggagtgt
     3961 atactggctt actatgttgg cactgatgag ggtgtcagtg aagtgcttca tgtggcagga
     4021 gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct
     4081 cgctcactga ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac
     4141 ggggcggaga tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc
     4201 ggcaaagccg tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc
     4261 aaatcagtgg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg
     4321 ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg
     4381 gccgcgtttg tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc
     4441 tggactgtat gcacgaaccc cccgttcagt ccgaccgctg cgccttatcc ggtaactatc
     4501 gtcttgagtc caacccggaa agacatgcaa aagcaccact ggcagcagcc actggtaatt
     4561 gatttagagg agttagtctt gaagtcatgc gccggttaag gctaaactga aaggacaagt
     4621 tttggtgact gcgctcctcc aagccagtta cctcggttca aagagttggt agctcagaga
     4681 accttcgaaa aaccgccctg caaggcggtt ttttcgtttt cagagcaaga gattacgcgc
     4741 agaccaaaac gatctcaaga agatcatctt attaatcaga taaaatattt ctagatttca
     4801 gtgcaattta tctcttcaaa tgtagcacct gaagtcagcc ccatacgata taagttgtaa
     4861 ttctcatgtt tgacagctta tcatcgatgc ataatgtgcc tgtcaaatgg acgaagcagg
     4921 gattctgcaa accctatgct actccgtcaa gccgtcaatt gtctgattcg ttaccaatta
     4981 tgacaacttg acggctacat cattcacttt ttcttcacaa ccggcacgga actcgctcgg
     5041 gctggccccg gtgcattttt taaatacccg cgagaaatag agttgatcgt caaaaccaac
     5101 attgcgaccg acggtggcga taggcatccg ggtggtgctc aaaagcagct tcgcctggct
     5161 gatacgttgg tcctcgcgcc agcttaagac gctaatccct aactgctggc ggaaaagatg
     5221 tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg ctggcgatat caaaattgct
     5281 gtctgccagg tgatcgctga tgtactgaca agcctcgcgt acccgattat ccatcggtgg
     5341 atggagcgac tcgttaatcg cttccatgcg ccgcagtaac aattgctcaa gcagatttat
     5401 cgccagcagc tccgaatagc gcccttcccc ttgcccggcg ttaatgattt gcccaaacag
     5461 gtcgctgaaa tgcggctggt gcgcttcatc cgggcgaaag aaccccgtat tggcaaatat
     5521 tgacggccag ttaagccatt catgccagta ggcgcgcgga cgaaagtaaa cccactggtg
     5581 ataccattcg cgagcctccg gatgacgacc gtagtgatga atctctcctg gcgggaacag
     5641 caaaatatca cccggtcggc aaacaaattc tcgtccctga tttttcacca ccccctgacc
     5701 gcgaatggtg agattgagaa tataaccttt cattcccagc ggtcggtcga taaaaaaatc
     5761 gagataaccg ttggcctcaa tcggcgttaa acccgccacc agatgggcat taaacgagta
     5821 tcccggcagc aggggatcat tttgcgcttc agccatactt ttcatactcc cgccattcag
     5881 ag
//
