LOCUS       sequence-33371-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Shida T, Cueva JG, Xu Z, Goodman MB, Nachury MV
  TITLE     The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes 
            rapid ciliogenesis and efficient mechanosensation.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21517-22. doi: 
            10.1073/pnas.1013728107. Epub 2010 Nov 10.
  PUBMED    21068373
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1013728107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-12-14- 14"; volume: "107"; issue: "50"; pages: 
            "21517-22"
COMMENT     Sequence Label: pEF5B-FRT-GFP-alpha-TAT1
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..42
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             55..99
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    136..160
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agctgtcagg gcgggggcgg ggagaatctt tattttcagg gcggcggggg cgggaaagaa
       61 accgctgctg ctaaattcga acgccagcac atggacagcg gactagtcca gtgtggtgga
      121 attctgcaga tatcaacaag tttgtacaaa aaagttgatg agttcccgtt cgatgtggac
      181 gcgctgttcc cggagcggat cacggtgctg gaccagcacc tgaggccccc agcccgccga
      241 cccggaacca caacgccggc ccgtgttgat ctacagcagc aaattatgac cattatagat
      301 gaactgggca aggcttctgc caaggcccag aatctttccg ctcctatcac tagtgcatca
      361 aggatgcaga gtaaccgcca tgttgtttat attctcaaag acagttcagc ccgaccggct
      421 ggaaaaggag ccattattgg tttcatcaaa gttggataca agaagctctt tgtactggat
      481 gatcgtgagg ctcataatga ggtagaacca ctttgcatcc tggactttta catccatgag
      541 tctgtgcaac gccatggcca tgggcgagaa ctcttccagt atatgttgca gaaggagcga
      601 gtggaaccgc accaactggc aattgaccga ccctcacaga agctgctgaa attcctgaat
      661 aagcactaca atctggagac cacagtccca caggtgaaca actttgtgat ctttgaaggc
      721 ttctttgccc atcaacatcg gccccctgct ccctctctga gggcaactcg acactctcgt
      781 gctgctgcag tcgatcccac gcccgctgct ccagcaagga agctgccacc caagagagca
      841 gagggagaca tcaag
//
