LOCUS       sequence-333719-       12720 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA tagged, codon Optimized and siRNA-resistant, 
            tetracycline-inducible KRASG12V sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12720)
  AUTHORS   Yuan TL, Fellmann C, Lee CS, Ritchie CD, Thapar V, Lee LC, Hsu DJ, 
            Grace D, Carver JO, Zuber J, Luo J, McCormick F, Lowe SW
  TITLE     Development of siRNA payloads to target KRAS-mutant cancer.
  JOURNAL   Cancer Discov. 2014 Oct;4(10):1182-1197. doi: 
            10.1158/2159-8290.CD-13-0900. Epub 2014 Aug 6.
  PUBMED    25100204
REFERENCE   2  (bases 1 to 12720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-13-0900"; journalName: "Cancer Discov"; date: 
            "2014-10"; volume: "4"; issue: "10"; pages: "1182-1197"
COMMENT     Sequence Label: pInducer10b-HA-KRAS G12V CO SR
FEATURES             Location/Qualifiers
     source          1..12720
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2384..2410
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        3009..4220
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4057..4076
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4227..4260
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4267..4884,4885..4890,4891..4926,4927..4929,4930..4965,
                     4966..4968,4969..5004,5005..5013)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5062..5634
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5229..5246)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5456..5475
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5637..6239
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6136..6156
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6261..6849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6314..6334)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6732..6743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7056..7289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7390..7407)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7396..7620
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7666..8094
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7753..7772)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7963..7984
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8103..8123)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8108..8437
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8288..8423
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8350..8369
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9260..9381
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9430..9446)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9430..9446)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9443..9465)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9454..9470
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9478..9484,9485..9502,9503..9508))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9523..9544
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9661..9678)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9832..10420)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9912..9931)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10591..11382,11383..11451))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11214..11233
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11452..11556)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    11604..11738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             11906..12539
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    12586..12711
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tagatctacc atgtacccat acgatgttcc
     2401 agattacgct ggaggatcag gaatgactga atataaactt gtggtggttg gagctgttgg
     2461 cgtgggcaag agtgccctaa caatccaact catccaaaac cattttgtgg acgaatatga
     2521 tccaacaata gaggattcct acaggaagca agtcgtgatt gatggagaaa cctgtctctt
     2581 ggatattctc gacacagccg ggcaagaaga atacagcgca atgagggacc agtacatgag
     2641 gactggggag ggctttcttt gtgtgtttgc cataaataat actaaatcat ttgaagatat
     2701 tcaccattat agagaacaaa ttaaaagagt taaggactct gaagatgtcc ccatggtgct
     2761 cgtgggcaat aaatgtgatt tgccttctag aacagtggac acaaaacaag cgcaagactt
     2821 agccagaagt tatggaattc cttttattga aacatcagca aagacaagac agggtgttga
     2881 tgatgccttc tatacattag ttcgagaaat tcgaaaacat aaagaaaaga tgagcaaaga
     2941 tggtaaaaag aagaaaaaga agtcaaagac aaagtgtgtc attatgtaac tcgagacgcg
     3001 taagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     3061 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     3121 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     3181 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     3241 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     3301 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     3361 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     3421 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     3481 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     3541 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     3601 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     3661 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     3721 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     3781 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     3841 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     3901 taggtgtgtg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     3961 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     4021 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     4081 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     4141 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     4201 ttttggcttt tttgttagac ggatccataa cttcgtataa tgtatgctat acgaagttat
     4261 gccaccatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     4321 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     4381 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     4441 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     4501 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     4561 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     4621 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     4681 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     4741 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     4801 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     4861 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     4921 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     4981 gacgattttg accttgacat gctccccggg taaataactt cgtatagcat acattatacg
     5041 aagtatggcg cgccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     5101 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5161 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5221 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     5281 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5341 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5401 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     5461 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     5521 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     5581 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
     5641 ccaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     5701 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     5761 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     5821 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     5881 gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt
     5941 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6001 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6061 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg
     6121 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6181 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat
     6241 taattaagcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6301 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6361 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6421 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6481 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6541 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6601 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     6661 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6721 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6781 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6841 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     6901 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6961 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7021 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     7081 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7141 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7201 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7261 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7321 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc
     7381 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc cccttccccg
     7441 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     7501 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     7561 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     7621 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     7681 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7741 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7801 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7861 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7921 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7981 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8041 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8101 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8161 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8221 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8281 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8341 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8401 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     8461 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtgaccgca
     8521 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     8581 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     8641 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     8701 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     8761 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     8821 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     8881 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     8941 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     9001 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     9061 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     9121 aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9181 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9241 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     9301 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9361 caaactaatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     9421 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9481 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     9541 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     9601 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     9661 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     9721 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     9781 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     9841 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     9901 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     9961 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10021 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10081 ctgtgtgcac taaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10141 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10201 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10261 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10321 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10381 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10441 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10501 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    10561 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    10621 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    10681 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    10741 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    10801 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    10861 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    10921 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    10981 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11041 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11101 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11161 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11221 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11281 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11341 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11401 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11461 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    11521 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    11581 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    11641 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    11701 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    11761 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    11821 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    11881 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    11941 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    12001 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12061 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    12121 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    12181 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    12241 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    12301 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    12361 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    12421 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    12481 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    12541 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    12601 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    12661 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
//
