LOCUS       sequence-33372-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Shida T, Cueva JG, Xu Z, Goodman MB, Nachury MV
  TITLE     The major alpha-tubulin K40 acetyltransferase alphaTAT1 promotes 
            rapid ciliogenesis and efficient mechanosensation.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21517-22. doi: 
            10.1073/pnas.1013728107. Epub 2010 Nov 10.
  PUBMED    21068373
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1013728107"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2010-12-14- 14"; volume: "107"; issue: "50"; pages: 
            "21517-22"
COMMENT     Sequence Label: pEF5B-FRT-GFP-alpha-TAT1[D157N]
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             59..103
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    140..164
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggagagctgt cagggcgggg gcggggagaa tctttatttt cagggcggcg ggggcgggaa
       61 agaaaccgct gctgctaaat tcgaacgcca gcacatggac agcggactag tccagtgtgg
      121 tggaattctg cagatatcaa caagtttgta caaaaaagtt gatgagttcc cgttcgatgt
      181 ggacgcgctg ttcccggagc ggatcacggt gctggaccag cacctgaggc ccccagcccg
      241 ccgacccgga accacaacgc cggcccgtgt tgatctacag cagcaaatta tgaccattat
      301 agatgaactg ggcaaggctt ctgccaaggc ccagaatctt tccgctccta tcactagtgc
      361 atcaaggatg cagagtaacc gccatgttgt ttatattctc aaagacagtt cagcccgacc
      421 ggctggaaaa ggagccatta ttggtttcat caaagttgga tacaagaagc tctttgtact
      481 ggatgatcgt gaggctcata atgaggtaga accactttgc atcctggact tttacatcca
      541 tgagtctgtg caacgccatg gccatgggcg agaactcttc cagtatatgt tgcagaagga
      601 gcgagtggaa ccgcaccaac tggcaattaa ccgaccctca cagaagctgc tgaaattcct
      661 gaataagcac tacaatctgg agaccacagt cccacaggtg aacaactttg tgatctttga
      721 aggcttcttt gcccatcaac atcggccccc tgctccctct ctgagggcaa ctcgacactc
      781 tcgtgctgct gcagtcgatc ccacgcccgc tgctccagca aggaagctgc cacccaagag
      841 agcagaggga gacatcaagc catactcctc tagtgaccga gaatttctga aggtagctgt
      901 ggagcctcct tggcccctaa caggcctgc
//
