LOCUS       sequence-33374-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt L1L3 tTA-3
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(56..289)
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 gggtccccaa actcaccctg aagttctcag ctctagagga tcctcgcgcc ccctacccac
       61 cgtactcgtc aattccaagg gcatcggtaa acatctgctc aaactcgaag tcggccatat
      121 ccagagcgcc gtagggggcg gagtcgtggg gggtaaatcc cggacccggg gaatccccgt
      181 cccccaacat gtccagatcg aaatcgtcta gcgcgtcggc atgcgccatc gccacgtcct
      241 cgccgtctaa gtggagctcg tcccccaggc tgacatcggt cgggggggcc gtcgacagtc
      301 tgcgcgtgtg tcccgcgggg agaaaggaca ggcgcggagc cgccagcccc gcctcttcgg
      361 gggcgtcgtc gtccgggaga tcgagcaggc cctcgatggt agacccgtaa ttgtttttcg
      421 tacgcgcgcg gctgtacgcg gacccacttt cacatttaag ttgtttttct aatccgcata
      481 tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga
      541 tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag
      601 cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc aca
//
