LOCUS       sequence-333740-       10101 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of cRaf fused to mNeonGreen-eDHFR(69K6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10101)
  AUTHORS   Suzuki S, Nakamura A, Hatano Y, Yoshikawa M, Yoshii T, Sawada S, 
            Atsuta-Tsunoda K, Aoki K, Tsukiji S
  TITLE     A chemogenetic platform for controlling plasma membrane signaling 
            and synthetic signal oscillation.
  JOURNAL   Cell Chem Biol. 2022 Sep 15;29(9):1446-1464.e10. doi: 
            10.1016/j.chembiol.2022.06.005. Epub 2022 Jul 13.
  PUBMED    35835118
REFERENCE   2  (bases 1 to 10101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2022.06.005"; journalName: "Cell Chem Biol"; 
            date: "2022-09-15- 15"; volume: "29"; issue: "9"; pages: 
            "1446-1464.e10"
COMMENT     Sequence Label: pPBpuro-cRaf-mNG-eDHFR(69K6)
FEATURES             Location/Qualifiers
     source          1..10101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   674..708
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1011..1390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1393..1668
                     /label=chicken beta-actin promoter
     intron          1669..2677
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2601..2623
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      4693..4702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4699..5406
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      4954..4963
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6008..6556
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6152..6169)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6379..6398
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6559..7158
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(6559..6578)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7055..7075
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7185..7202)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7191..7415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7462..7478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7486..7502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7510..7516,7517..7534,7535..7540))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7555..7576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     repeat_region   7810..7872
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(7913..7933)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7913..7931)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7952..7968)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7976..7992
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8000..8006,8007..8024,8025..8030))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8045..8066
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8183..8200)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8354..8942)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8434..8453)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9113..9904,9905..9973))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9736..9755
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9974..10078)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggggcgcgc
      661 cattctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
      721 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      781 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      841 ataacgaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      901 actcatacga taattatatt gttatttcat gttctactta cgtgataact tattatatat
      961 atattttctt gttatagata tcaactagaa tgctagcatg ggcccatctc gacattgatt
     1021 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1081 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     1141 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     1201 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     1261 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     1321 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     1381 tattaccatg ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
     1441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
     1501 gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg
     1561 cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg
     1621 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg
     1681 ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc gcccgccccg gctctgactg
     1741 accgcgttac tcccacaggt gagcgggcgg gacggccctt ctcctccggg ctgtaattag
     1801 cgcttggttt aatgacggct cgtttctttt ctgtggctgc gtgaaagcct taaagggctc
     1861 cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     1921 ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg agcgctgcgg gcgcggcgcg
     1981 gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc cgggggcggt gccccgcggt
     2041 gcgggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg gggggtgagc
     2101 agggggtgtg ggcgcggcgg tcgggctgta acccccccct gcacccccct ccccgagttg
     2161 ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg gcgtggcgcg gggctcgccg
     2221 tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg cctcgggccg
     2281 gggagggctc gggggagggg cgcggcggcc ccggagcgcc ggcggctgtc gaggcgcggc
     2341 gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac ttcctttgtc
     2401 ccaaatctgg cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg
     2461 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     2521 gccgccgtcc ccttctccat ctccagcctc ggggctgccg cagggggacg gctgccttcg
     2581 ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct ctagagcctc
     2641 tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttgt
     2701 tgtgctgtct catcattttg gcaaagaatt gggatcatgg agcacataca gggagcttgg
     2761 aagacgatca gcaatggttt tggattcaaa gatgccgtgt ttgatggctc cagctgcatc
     2821 tctcctacaa tagttcagca gtttggctat cagcgccggg catcagatga tggcaaactc
     2881 acagatcctt ctaagacaag caacactatc cgtgttttct tgccgaacaa gcaaagaaca
     2941 gtggtcaatg tgcgaaatgg aatgagcttg catgactgcc ttatgaaagc actcaaggtg
     3001 aggggcctgc aaccagagtg ctgtgcagtg ttcagacttc tccacgaaca caaaggtaaa
     3061 aaagcacgct tagattggaa tactgatgct gcgtctttga ttggagaaga acttcaagta
     3121 gatttcctgg atcatgttcc cctcacaaca cacaactttg ctcggaagac gttcctgaag
     3181 cttgccttct gtgacatctg tcagaaattc ctgctcaatg gatttcgatg tcagacttgt
     3241 ggctacaaat ttcatgagca ctgtagcacc aaagtaccta ctatgtgtgt ggactggagt
     3301 aacatcagac aactcttatt gtttccaaat tccactattg gtgatagtgg agtcccagca
     3361 ctaccttctt tgactatgcg tcgtatgcga gagtctgttt ccaggatgcc tgttagttct
     3421 cagcacagat attctacacc tcacgccttc acctttaaca cctccagtcc ctcatctgaa
     3481 ggttccctct cccagaggca gaggtcgaca tccacaccta atgtccacat ggtcagcacc
     3541 accctgcctg tggacagcag gatgattgag gatgcaattc gaagtcacag cgaatcagcc
     3601 tcaccttcag ccctgtccag tagccccaac aatctgagcc caacaggctg gtcacagccg
     3661 aaaacccccg tgccagcaca aagagagcgg gcaccagtat ctgggaccca ggagaaaaac
     3721 aaaattaggc ctcgtggaca gagagattca agctattatt gggaaataga agccagtgaa
     3781 gtgatgctgt ccactcggat tgggtcaggc tcttttggaa ctgtttataa gggtaaatgg
     3841 cacggagatg ttgcagtaaa gatcctaaag gttgtcgacc caaccccaga gcaattccag
     3901 gccttcagga atgaggtggc tgttctgcgc aaaacacggc atgtgaacat tctgcttttc
     3961 atggggtaca tgacaaagga caacctggca attgtgaccc agtggtgcga gggcagcagc
     4021 ctctacaaac acctgcatgt ccaggagacc aagtttcaga tgttccagct aattgacatt
     4081 gcccggcaga cggctcaggg aatggactat ttgcatgcaa agaacatcat ccatagagac
     4141 atgaaatcca acaatatatt tctccatgaa ggcttaacag tgaaaattgg agattttggt
     4201 ttggcaacag taaagtcacg ctggagtggt tctcagcagg ttgaacaacc tactggctct
     4261 gtcctctgga tggccccaga ggtgatccga atgcaggata acaacccatt cagtttccag
     4321 tcggatgtct actcctatgg catcgtattg tatgaactga tgacggggga gcttccttat
     4381 tctcacatca acaaccgaga tcagatcatc ttcatggtgg gccgaggata tgcctcccca
     4441 gatcttagta agctatataa gaactgcccc aaagcaatga agaggctggt agctgactgt
     4501 gtgaagaaag taaaggaaga gaggcctctt tttccccaga tcctgtcttc cattgagctg
     4561 ctccaacact ctctaccgaa gatcaaccgg agcgcttccg agccatcctt gcatcgggca
     4621 gcccacactg aggatatcaa tgcttgcacg ctgaccacgt ccccgaggct gcctgtcttc
     4681 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc ctctctccca
     4741 gcgacacatg agttacacat ctttggctcc atcaacggtg tggactttga catggtgggt
     4801 cagggcaccg gcaatccaaa tgatggttat gaggagttaa acctgaagtc caccaagggt
     4861 gacctccagt tctccccctg gattctggtc cctcatatcg ggtatggctt ccatcagtac
     4921 ctgccctacc ctgacgggat gtcgcctttc caggccgcca tggtagatgg ctccggctac
     4981 caagtccatc gcacaatgca gtttgaagat ggtgcctccc ttactgttaa ctaccgctac
     5041 acctacgagg gaagccacat caaaggagag gcccaggtga aggggactgg tttccctgct
     5101 gacggtcctg tgatgaccaa ctcgctgacc gctgcggact ggtgcaggtc gaagaagact
     5161 taccccaacg acaaaaccat catcagtacc tttaagtgga gttacaccac tggaaatggc
     5221 aagcgctacc ggagcactgc gcggaccacc tacacctttg ccaagccaat ggcggctaac
     5281 tatctgaaga accagccgat gtacgtgttc cgtaagacgg agctcaagca ctccaagacc
     5341 gagctcaact tcaaggagtg gcaaaaggcc tttaccgatg tgatgggcat ggacgagctg
     5401 tacaagtctg ggtccgcagc cagtagcgcc ggaagtatga tcagtctgat tgcggcgtta
     5461 gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc
     5521 gcctggttta aacgcaacac cttaaataaa cccgtgatta tgggccgcca tacctgggaa
     5581 tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg
     5641 gacaaaaaaa agaaaaagaa agatcgcgta acgtgggtga agtcggtgga tgaagccatc
     5701 gcggcgtgtg gtgacgtacc agaaatcatg gtgattggcg gcggtcgcgt ttatgaacag
     5761 ttcttgccaa aagcgcaaaa actgtatctg acgcatatcg acgcagaagt ggaaggcgac
     5821 acccatttcc cggattacga gccggatgac tgggaatcgg tattcagcga attccacgat
     5881 gctgatgcgc agaactctca cagctattgc tttgagattc tggagcggcg gtaacccggg
     5941 ataagtcaac taacttaagc tagcaacggt ttccctctag cgggatcaat tccgcccccc
     6001 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     6061 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     6121 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     6181 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     6241 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     6301 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     6361 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     6421 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     6481 gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt
     6541 gaaaaacacg ataataccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6601 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6661 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6721 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6781 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6841 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6901 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6961 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     7021 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     7081 ggcttcaccg tcaccgccga cgtcgagtgc ccgaaggacc gcgcgacctg gtgcatgacc
     7141 cgcaagcccg gtgcctgatc gatgatctag agctcgctga tcagcctcga ctgtgccttc
     7201 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     7261 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     7321 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     7381 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaacctgcag
     7441 cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7501 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7561 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7621 gtgccagcgg atcttaaaag ttttgttact ttatagaaga aattttgagt ttttgttttt
     7681 ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta gtatgtaagt
     7741 gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga tatacagacc
     7801 gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca caatatgatt
     7861 atctttctag ggttaatcta gtatacgcgt ttaattaatc cagcttttgt tccctttagt
     7921 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7981 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8041 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8101 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8161 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8761 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9121 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9181 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9241 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9301 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9361 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9421 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9481 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9541 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9601 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9661 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9721 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9781 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9841 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9901 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9961 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10021 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10081 catttccccg aaaagtgcca c
//
