LOCUS       sequence-33375-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: Exp-pcDNA3.2delCMV(EF1-alpha-tTA/TetO-mCh-Rs1)
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(39..419)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(101..122)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     313..332
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    621..669
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     694..713
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(753..794)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gtaatttttg ttaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
       61 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      121 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
      181 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
      241 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
      301 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
      361 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg
      421 gggatacccc ctagagcccc agctgcgcag atctgctatg gcagggcctg ccgccccgac
      481 gttggctgcg agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga
      541 aacgcgggcg tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg
      601 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
      661 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
      721 tcccccgttt aaactcatta ctaaccggta cgcgtagaat cgagaccgag gagagggtta
      781 gggataggct taccttcgaa ccgcgggccc tctagatcaa ccactttgta
//
