LOCUS       sequence-333752-        9325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid that encodes mEmerald-CD9 fusion protein with 
            microRNA 9 tandem cassette to restrict expression to microglia with 
            EF1-alpha promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9325)
  AUTHORS   Clayton K, Delpech JC, Herron S, Iwahara N, Ericsson M, Saito T, 
            Saido TC, Ikezu S, Ikezu T
  TITLE     Plaque associated microglia hyper-secrete extracellular vesicles and
            accelerate tau propagation in a humanized APP mouse model.
  JOURNAL   Mol Neurodegener. 2021 Mar 22;16(1):18. doi: 
            10.1186/s13024-021-00440-9.
  PUBMED    33752701
REFERENCE   2  (bases 1 to 9325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2021-03-22"; volume: "16(1):18. doi"; pages: 
            " 10.1186/s13024-021-00440-9"
COMMENT     Sequence Label: pLV.EF1-alpha.mEmerald.CD9.miR9T
FEATURES             Location/Qualifiers
     source          1..9325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    205..322
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        420..631
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          632..1570
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1527..1547
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1593..1602
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1599..1601,1602..1604,1605..2315)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1644..1665)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1905..1924)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2252..2273
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3111..3131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3529..3540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3803..4036
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4108..4229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4145..4164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4199..4218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4269..4404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4424..4443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4425..4443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4453..4470)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4453..4469)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4462..4484)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4611..5066
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4698..4717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4908..4929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5092..5196
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5197..5265,5266..6057)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5415..5434)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6228..6816
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6717..6736
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6970..6987
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7104..7125
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7140..7145,7146..7163,7164..7170)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7178..7194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7183..7205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7202..7218
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7202..7218
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7237..7257
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7239..7257
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        7358..7737
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7738..7941
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7892..7912
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7956..8136
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8183..8308
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8801..9034
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9219..9263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
       61 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
      121 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
      181 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
      241 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      301 aattacaaaa attcaaaatt ttatcgatca cgagactagc ctcgagaagc ttgatatcga
      361 attcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
      421 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
      481 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
      541 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
      601 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
      661 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
      721 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
      781 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
      841 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
      901 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
      961 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     1021 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     1081 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     1141 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     1201 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     1261 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     1321 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     1381 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     1441 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     1501 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     1561 tccatttcag gtgtcgtgag gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
     1621 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1681 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1741 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttgacctacg
     1801 gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1861 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1921 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     1981 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     2041 gccacaaggt ctatatcacc gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     2101 cccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2161 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccaagc
     2221 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2281 ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct cgagctcaag
     2341 cttccccggt caaaggaggc accaagtgca tcaaatacct gctgttcgga tttaacttca
     2401 tcttctggct tgccgggatt gctgtccttg ccattggact atggctccga ttcgactctc
     2461 agaccaagag catcttcgag caagaaacta ataataataa ttccagcttc tacacaggag
     2521 tctatattct gatcggagcc ggcgccctca tgatgctggt gggcttcctg ggctgctgcg
     2581 gggctgtgca ggagtcccag tgcatgctgg gactgttctt cggcttcctc ttggtgatat
     2641 tcgccattga aatagctgcg gccatctggg gatattccca caaggatgag gtgattaagg
     2701 aagtccagga gttttacaag gacacctaca acaagctgaa aaccaaggat gagccccagc
     2761 gggaaacgct gaaagccatc cactatgcgt tgaactgctg tggtttggct gggggcgtgg
     2821 aacagtttat ctcagacatc tgccccaaga aggacgtact cgaaaccttc accgtgaagt
     2881 cctgtcctga tgccatcaaa gaggtcttcg acaataaatt ccacatcatc ggcgcagtgg
     2941 gcatcggcat tgccgtggtc atgatatttg gcatgatctt cagtatgatc ttgtgctgtg
     3001 ctatccgcag gaaccgcgag atggtctagg gatccaaggg cgaattctgc agtcgacaat
     3061 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3121 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3181 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3241 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3301 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3361 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3421 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     3481 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3541 ccagcggacc ttccttcccg cggcctgctg ccggctctag agtcatacag ctagataacc
     3601 aaagacgtat catacagcta gataaccaaa gagctagagt catacagcta gataaccaaa
     3661 gacgtatcat acagctagat aaccaaagag ctagagcctc ttccgcgtct tcgccttccc
     3721 gggtcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3781 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     3841 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4021 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     4081 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     4141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4201 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     4261 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     4321 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     4381 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     4441 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4501 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4561 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4621 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4681 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4741 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4801 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4861 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4921 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4981 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5041 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     5101 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     5161 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     5221 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     5281 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     5341 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     5401 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     5461 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     5521 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     5581 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     5641 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     5701 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     5761 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     5821 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     5881 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     5941 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     6001 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     6061 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6121 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6181 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6241 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6301 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6361 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6421 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6481 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6541 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6601 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     6661 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     6721 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     6781 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     6841 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     6901 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     6961 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     7021 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     7081 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     7141 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     7201 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     7261 caaaagctgg agctgcaagc ttggccattg catacgttgt atccatatca taatatgtac
     7321 atttatattg gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat
     7381 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     7441 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     7501 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     7561 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     7621 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     7681 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg
     7741 atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca
     7801 agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt
     7861 ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg
     7921 gaggtctata taagcagagc tcgtttagtg aaccggggtc tctctggtta gaccagatct
     7981 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8041 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8101 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     8161 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     8221 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     8281 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     8341 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     8401 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     8461 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     8521 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8581 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8641 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8701 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8761 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8821 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8881 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8941 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     9001 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     9061 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     9121 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     9181 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     9241 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     9301 taaaattatt cataatgata gtagg
//
