LOCUS       sequence-33376-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: Exp-pcDNA3.2delCMV(EF1-alpha-tTA/TetO-mCh-Rs1)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             269..325
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gtccccgaag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
       61 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      121 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      181 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      241 caagacctgg tttctcgagg gcagcggcgc gaccaacttt agcctgctga aacaggcggg
      301 cgatgtggaa gaaaacccag gaccgaccgg tatcaagacg atcatcgccc tgagctacat
      361 cttctgcctg gtattcgccg actacaagga cgatgatgac gccagcggcc gcgcccgggc
      421 ggatcccccg ggctgcagga tggacaaact tgatgctaat gtgagttctg aggagggttt
      481 cgggtcagtg gagaaggtgg tgctgctcac gtttctctcg acggttatcc tgatggccat
      541 cttggggaac ctgctggtga tggtggctgt gtgctgggac aggcagctca ggaaaataaa
      601 aacaaattat ttcattgtat ctcttgcttt tgcggatctg ctggtttcgg tgctggtgat
      661 gccctttggt gccattgagc tggttcaaga catctggatt tatggggagg tgttttgtct
      721 tgttcggaca tctctggccg tcctgctcac aacggcatcg atttttcacc tgtgctgcat
      781 ttctctggat aggtattacg ccatctgctg ccagcctttg gtctatagga acaagatgac
      841 ccttnctcct atgtgagtgt gttccctatc
//
