LOCUS       sequence-333789-        2310 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 0 transcription terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Gale GAR, Wang B, McCormick AJ
  TITLE     Evaluation and Comparison of the Efficiency of Transcription 
            Terminators in Different Cyanobacterial Species.
  JOURNAL   Front Microbiol. 2021 Jan 15;11:624011. doi: 
            10.3389/fmicb.2020.624011. eCollection 2020.
  PUBMED    33519785
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Microbiol."; date: "2021-01-15"; volume: "11:624011. doi"; pages: " 
            10.3389/fmicb.2020.624011. eCollection 2020"
COMMENT     Sequence Label: pC0.292 L3S2P11
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(17..34)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(188..776)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(268..287)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(947..1738)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1960..2064)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2132..2150
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
       61 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
      121 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
      181 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
      241 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
      301 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
      361 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
      421 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
      481 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
      541 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
      601 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
      661 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
      721 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
      781 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
      841 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
      901 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttat ttgccgacta
      961 ccttggtgat ctcgcctttt acgtaatgga caaattcttc caactgatct gcgcgcgagg
     1021 ccaagcgatc ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat
     1081 actgggccgg caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc
     1141 cggttactgc gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag
     1201 cccagtcggg cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct
     1261 gttcaggaac cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt
     1321 ctcttgcttt tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac
     1381 ctgcaagaat gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac
     1441 gccacggaat gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc
     1501 tctctccagg ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt
     1561 catcaagcct tacggtcacc gtaaccagca aatcaatatc gctgtgtggc ttcaggccgc
     1621 catccactgc ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga
     1681 tgacgccaac tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcatga
     1741 tgtttaactt tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca
     1801 tcaaacatcg acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta
     1861 ccccaaaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
     1921 gcgttcggtc aaggttctgg accagttgcg tgagcgcata ctcttccttt ttcaatatta
     1981 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2041 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2101 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtcc
     2161 actgaagagc cacttcgtgg tctcaagcgg gaccaaaacg aaaaaagacg ctcgaaagcg
     2221 tctcttttct ggaatttggt accgagaagc tgagaccaca gagtgattaa tgaatcggcc
     2281 aacgcgcggg gagaggcggt ttgcgtattg
//
