LOCUS       sequence-333796-       13330 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13330)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 13330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED
FEATURES             Location/Qualifiers
     source          1..13330
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2314..2332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2398..2416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2440..2458
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2491..2529
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2526..2550
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2659..2683
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3931..3987
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4237..4254
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4804..4857
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(5680..5704)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5757..5783
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        6003..7181
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6234..7172
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7129..7149
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7251..7284
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7291..7908,7909..7914,7915..7950,7951..7953,7954..7989,
                     7990..7992,7993..8028,8029..8037)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    8080..8542
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8136..8153)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8363..8382
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8558..8560,8561..8563,8564..9277)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8558..8560,8561..8563,8564..9274)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8603..8624)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8864..8883)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9211..9232
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9413..9432
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9720..10308
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9773..9793)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10191..10202)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10383..10616
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10735..10753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10821..10925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10926..10994,10995..11786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11144..11163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11957..12545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12446..12465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12699..12716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12956..12977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12992..12997,12998..13015,13016..13022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13030..13046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13035..13057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13054..13070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13054..13070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13120..13139
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     2401 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2461 agtgaaagtc gagctcggta cccgggtcga ggtaggcgtg tacggtggga ggcctatata
     2521 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
     2581 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt ccagtgtggt
     2641 ggaattctgc agatatcaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc
     2701 accatgcagc gaatgatcca acaatttgct gctgaatata cctcaaaaaa tagctctact
     2761 caggacccca gccagcccaa tagcacaaag aaccaaagcc tgccgaaagc atctccagtc
     2821 accacctctc ccacggctgc aactactcag aaccctgtgc tcagcaaact tctcatggct
     2881 gaccaagact cacctctgga ccttactgtc agaaagtctc agtcagaacc tagcgaacaa
     2941 gacggtgtac ttgatctgtc cactaagaaa agtccatgtg ctggcagcac ttccctgagc
     3001 cactctccag gctgctccag tactcaaggg aacgggcgac ctgggagacc cagccagtac
     3061 cgcccagacg gacttcggag tggtgatggg gtacctccaa gaagcttaca ggatggaacc
     3121 agggaaggtt ttggacactc cacatcactc aaagttccac tggctcgatc cctgcagatt
     3181 agtgaagaac tactgagcag aaaccaattg tccacagctg ccagccttgg gccatctgga
     3241 ttacagaatc atggacaaca cttaatatta tccagggaag cctcttgggc aaaaccacat
     3301 tacgagttca acctcagccg tatgaagttc aggggaaatg gtgcactcag caacatcagt
     3361 gaccttcctt ttcttgcaga aaactctgcc tttccaaaaa tggcacttca agcaaaacaa
     3421 gatggaaaaa aggatgtgag ccattcatct cctgtagatt taaagatacc acaagttcga
     3481 ggaatggatc tttcttggga gtctcgcact ggtgatcagt acagctatag ctctttggta
     3541 atgggttcac aaacggagag cgcgcttagt aaaaaattaa gggctattct tccaaaacaa
     3601 agtagaaaaa gcatgttaga tgctggaccc gattcttggg gctcagatgc tgagcagtct
     3661 acctctggac agccatatcc cacatcggat caagaaggag accctggctc caagcagcct
     3721 cggaagaaaa gagggcgtta cagacagtac aacagtgaga tactggagga agcaatctca
     3781 gtggttatga gtggaaaaat gagtgtttcc aaagctcaga gtatttatgg gattccccac
     3841 agtacactgg agtacaaagt aaaggagagg ctgggcactt tgaaaaaccc tccaaagaaa
     3901 aagatgaaat taatgagtgg aggatctgga gcaacaaact tctcactact caaacaagca
     3961 ggtgacgtgg aggagaatcc cggccctatg gccaccactg gggccctggg caactactac
     4021 gtggactcgt tcctgctggg cgccgacgcc gcggatgagc tgagcgttgg ccgctatgcg
     4081 ccggggaccc tgggccagcc tccccggcag gcggcgacgc tggccgagca ccccgacttc
     4141 agcccgtgca gcttccagtc caaggcgacg gtgtttggcg cctcgtggaa cccagtgcac
     4201 gcggcgggcg ccaacgctgt acccgctgcg gtgtaccacc accatcacca ccacccctac
     4261 gtgcaccccc aggcgcccgt ggcggcggcg gcgccggacg gcaggtacat gcgctcctgg
     4321 ctggagccca cgcccggtgc gctctccttc gcgggcttgc cctccagccg gccttatggc
     4381 attaaacctg aaccgctgtc ggccagaagg ggtgactgtc ccacgcttga cactcacact
     4441 ttgtccctga ctgactatgc ttgtggttct cctccagttg atagagaaaa acaacccagc
     4501 gaaggcgcct tctctgaaaa caatgctgag aatgagagcg gcggagacaa gccccccatc
     4561 gatcccaata acccagcagc caactggctt catgcgcgct ccactcggaa aaagcggtgc
     4621 ccctatacaa aacaccagac cctggaactg gagaaagagt ttctgttcaa catgtacctc
     4681 accagggacc gcaggtacga ggtggctcga ctgctcaacc tcaccgagag gcaggtcaag
     4741 atctggttcc agaaccgcag gatgaaaatg aagaaaatca acaaagaccg agcaaaagac
     4801 gaggagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctatg
     4861 agctcttatt ttgtaaactc attttgcggt cgctatccaa atggcccgga ctaccagttg
     4921 cataattatg gagatcatag ttccgtgagc gagcaattca gggactcggc gagcatgcac
     4981 tccggcaggt acggctacgg ctacaatggc atggatctca gcgtcggccg ctcgggctcc
     5041 ggccactttg gctccggaga gcgcgcccgc agctacgctg ccagcgccag cgcggcgccc
     5101 gccgagccca ggtacagcca gccggccacg tccacgcact ctcctcagcc cgatccgctg
     5161 ccctgctccg ccgtggcccc ctcgcccggc agcgacagcc accacggcgg gaaaaactcc
     5221 ctaagcaact ccagcggcgc ctcggccgac gccggcagca cccacatcag cagcagagag
     5281 ggggttggca cggcgtccgg agccgaggag gacgcccctg ccagcagcga gcaggcgagt
     5341 gcgcagagcg agccgagccc ggcgccgccc gcccaacccc agatctaccc ctggatgcgc
     5401 aagctgcaca taagtcatga caacataggc ggcccggaag gcaaaagggc ccggacggcc
     5461 tacacgcgct accagaccct ggagctggag aaggagttcc acttcaaccg ttacctgacc
     5521 cgcagaagga ggattgaaat agcacatgct ctttgcctct ccgagagaca aattaaaatc
     5581 tggttccaaa accggagaat gaagtggaaa aaagataata agctgaaaag catgagcatg
     5641 gccgcggcag gaggggcctt ccgtccctga ggcgcgccga cccagctttc ttgtacaaag
     5701 tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgcgg ttcgaatacc
     5761 catacgacgt cccagactac gcttagtaat gattaattaa actagaaatt ctaccgggta
     5821 ggggaggcgc ttttcccaag gcagtctgga gtaacccacc caagatctgg cctccgcgcc
     5881 gggttttggc gcctcccgcg ggcgcccccc tcctcacggc gagccgcgta agatctggcc
     5941 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcctagtcgt
     6001 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     6061 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     6121 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     6181 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     6241 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     6301 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     6361 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     6421 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     6481 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     6541 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     6601 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     6661 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     6721 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     6781 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     6841 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     6901 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     6961 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     7021 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     7081 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     7141 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc catgctagta
     7201 aattgtccgc taaattctgg ccgtttttgg cttttttgtt agacggatcc ataacttcgt
     7261 ataatgtatg ctatacgaag ttatgccacc atgtctagac tggacaagag caaagtcata
     7321 aacggagctc tggaattact caatggtgtc ggtatcgaag gcctgacgac aaggaaactc
     7381 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     7441 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactt ctgccccctg
     7501 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     7561 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     7621 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     7681 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     7741 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     7801 agacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     7861 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     7921 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     7981 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaacta
     8041 agtaaggatc cgcggccgca ctagaggaat tccgcccctg agggcccgga aacctggccc
     8101 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     8161 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     8221 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     8281 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     8341 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     8401 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac
     8461 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     8521 cctttgaaaa acacgatgat aagcttaccg gtccaccatg gtgagcaagg gcgaggagct
     8581 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     8641 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     8701 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     8761 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     8821 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     8881 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     8941 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     9001 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     9061 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     9121 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     9181 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     9241 cgggatcact ctcggcatgg acgagctgta caagtaagca tgcccgacgg cgaggatctc
     9301 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
     9361 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
     9421 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
     9481 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
     9541 tgatgtacaa gtaaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     9601 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     9661 tgcaattgtt gttgtttagt ccctcccaat tcgatatcaa gcttatcgcg atagatccta
     9721 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     9781 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     9841 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     9901 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     9961 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
    10021 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
    10081 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
    10141 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
    10201 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
    10261 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct gagatccttt
    10321 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
    10381 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
    10441 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    10501 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    10561 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
    10621 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
    10681 tgagaggccc gggttaatta aggaaagggc tagatcattc ttgaagacga aagggcctcg
    10741 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
    10801 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10861 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10921 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10981 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    11041 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    11101 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    11161 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11221 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11281 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11341 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11401 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    11461 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11521 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    11581 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    11641 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11701 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11761 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11821 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11881 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11941 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    12001 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    12061 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    12121 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12181 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12241 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12301 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12361 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12421 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12481 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12541 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    12601 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    12661 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    12721 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    12781 gcaagctcat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    12841 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
    12901 ccccgtggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    12961 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    13021 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac atgattacga
    13081 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    13141 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
    13201 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
    13261 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
    13321 acttagtagt
//
