LOCUS       sequence-333798-       13334 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Improved version to express RUNX1, P2A, ERG in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Sugimura R, Jha DK, Han A, Soria-Valles C, da Rocha EL, Lu YF, 
            Goettel JA, Serrao E, Rowe RG, Malleshaiah M, Wong I, Sousa P, Zhu 
            TN, Ditadi A, Keller G, Engelman AN, Snapper SB, Doulatov S, Daley 
            GQ
  TITLE     Haematopoietic stem and progenitor cells from human pluripotent stem
            cells.
  JOURNAL   Nature. 2017 May 17. doi: 10.1038/nature22370.
  PUBMED    28514439
REFERENCE   2  (bases 1 to 13334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2017 May 17. doi: 10.1038/nature22370."
COMMENT     Sequence Label: pINDUCER-21-RUNX1-P2A-ERG-IMPROVED
FEATURES             Location/Qualifiers
     source          1..13334
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2230..2248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2272..2290
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2314..2332
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2407..2445
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2442..2466
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2575..2599
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4072..4128
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(5578..5602)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5655..5681
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5901..7079
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6132..7070
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7027..7047
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    7149..7182
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(7189..7806,7807..7812,7813..7848,7849..7851,7852..7887,
                     7888..7890,7891..7926,7927..7935)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    7973..8546
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8140..8157)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8367..8386
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(8562..8564,8565..8567,8568..9281)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8562..8564,8565..8567,8568..9278)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8607..8628)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8868..8887)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9215..9236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9417..9436
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9724..10312
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9777..9797)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10195..10206)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10387..10620
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10739..10757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10825..10929
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10930..10998,10999..11790)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11148..11167)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11961..12549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12450..12469
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12703..12720
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12960..12981
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12996..13001,13002..13019,13020..13026)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13034..13050
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13039..13061
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13058..13074
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13058..13074
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13124..13143
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactaggatc
     2221 ctttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     2281 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     2341 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagc tcggtacccg
     2401 ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
     2461 cagatcgcct ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga
     2521 tccagcctcc gcggccccga actagtccag tgtggtggaa ttctgcagat atcaacaagt
     2581 ttgtacaaaa aagcaggctc cgcggccgcc cccttcacca tggcttcaga cagcatattt
     2641 gagtcatttc cttcgtaccc acagtgcttc atgagagaat gcatacttgg aatgaatcct
     2701 tctagagacg tccacgatgc cagcacgagc cgccgcttca cgccgccttc caccgcgctg
     2761 agcccaggca agatgagcga ggcgttgccg ctgggcgccc cggacgccgg cgctgccctg
     2821 gccggcaagc tgaggagcgg cgaccgcagc atggtggagg tgctggccga ccacccgggc
     2881 gagctggtgc gcaccgacag ccccaacttc ctctgctccg tgctgcctac gcactggcgc
     2941 tgcaacaaga ccctgcccat cgctttcaag gtggtggccc taggggatgt tccagatggc
     3001 actctggtca ctgtgatggc tggcaatgat gaaaactact cggctgagct gagaaatgct
     3061 accgcagcca tgaagaacca ggttgcaaga tttaatgacc tcaggtttgt cggtcgaagt
     3121 ggaagaggga aaagcttcac tctgaccatc actgtcttca caaacccacc gcaagtcgcc
     3181 acctaccaca gagccatcaa aatcacagtg gatgggcccc gagaacctcg aagacatcgg
     3241 cagaaactag atgatcagac caagcccggg agcttgtcct tttccgagcg gctcagtgaa
     3301 ctggagcagc tgcggcgcac agccatgagg gtcagcccac accacccagc ccccacgccc
     3361 aaccctcgtg cctccctgaa ccactccact gcctttaacc ctcagcctca gagtcagatg
     3421 caggatacaa ggcagatcca accatcccca ccgtggtcct acgatcagtc ctaccaatac
     3481 ctgggatcca ttgcctctcc ttctgtgcac ccagcaacgc ccatttcacc tggacgtgcc
     3541 agcggcatga caaccctctc tgcagaactt tccagtcgac tctcaacggc acccgacctg
     3601 acagcgttca gcgacccgcg ccagttcccc gcgctgccct ccatctccga cccccgcatg
     3661 cactatccag gcgccttcac ctactccccg acgccggtca cctcgggcat cggcatcggc
     3721 atgtcggcca tgggctcggc cacgcgctac cacacctacc tgccgccgcc ctaccccggc
     3781 tcgtcgcaag cgcagggagg cccgttccaa gccagctcgc cctcctacca cctgtactac
     3841 ggcgcctcgg ccggctccta ccagttctcc atggtgggcg gcgagcgctc gccgccgcgc
     3901 atcctgccgc cctgcaccaa cgcctccacc ggctccgcgc tgctcaaccc cagcctcccg
     3961 aaccagagcg acgtggtgga ggccgagggc agccacagca actcccccac caacatggcg
     4021 ccctccgcgc gcctggagga ggccgtgtgg aggccctact tgggatctgg agcaacaaac
     4081 ttctcactac tcaaacaagc aggtgacgtg gaggagaatc ccggccctat ggccagcact
     4141 attaaggaag ccttatcagt tgtgagtgag gaccagtcgt tgtttgagtg tgcctacgga
     4201 acgccacacc tggctaagac agagatgacc gcgtcctcct ccagcgacta tggacagact
     4261 tccaagatga gcccacgcgt ccctcagcag gattggctgt ctcaaccccc agccagggtc
     4321 accatcaaaa tggaatgtaa ccctagccag gtgaatggct caaggaactc tcctgatgaa
     4381 tgcagtgtgg ccaaaggcgg gaagatggtg ggcagcccag acaccgttgg gatgaactac
     4441 ggcagctaca tggaggagaa gcacatgcca cccccaaaca tgaccacgaa cgagcgcaga
     4501 gttatcgtgc cagcagatcc tacgctatgg agtacagacc atgtgcggca gtggctggag
     4561 tgggcggtga aagaatatgg ccttccagac gtcaacatct tgttattcca gaacatcgat
     4621 gggaaggaac tgtgcaagat gaccaaggac gacttccaga ggctcacccc cagctacaat
     4681 gccgacatcc ttctctcaca tctccactac ctcagagaga ctcctcttcc acatttgact
     4741 tcagatgatg ttgataaagc cttacaaaac tctccacggt taatgcatgc tagaaacaca
     4801 gggggtgcag cttttatttt cccaaatact tcagtatatc ctgaagctac gcaaagaatt
     4861 acaactaggc cagatttacc atatgagccc cccaggagat cagcctggac cggtcacggc
     4921 caccccacgc cccagtcgaa agctgctcaa ccatctcctt ccacagtgcc caaaactgaa
     4981 gaccagcgtc ctcagttaga tccttatcag attcttggac caacaagtag ccgccttgca
     5041 aatccaggca gtggccagat ccagctttgg cagttcctcc tggagctcct gtcggacagc
     5101 tccaactcca gctgcatcac ctgggaaggc accaacgggg agttcaagat gacggatccc
     5161 gacgaggtgg cccggcgctg gggagagcgg aagagcaaac ccaacatgaa ctacgataag
     5221 ctcagccgcg ccctccgtta ctactatgac aagaacatca tgaccaaggt ccatgggaag
     5281 cgctacgcct acaagttcga cttccacggg atcgcccagg ccctccagcc ccaccccccg
     5341 gagtcatctc tgtacaagta cccctcagac ctcccgtaca tgggctccta tcacgcccac
     5401 ccacagaaga tgaactttgt ggcgccccac cctccagccc tccccgtgac atcttccagt
     5461 ttttttgctg ccccaaaccc atactggaat tcaccaactg ggggtatata ccccaacact
     5521 aggctcccca ccagccatat gccttctcat ctgggcactt actactgagg cgcgccgacc
     5581 cagctttctt gtacaaagtg gttgatatcc agcacagtgg cggccgctcg agtctagagg
     5641 gcccgcggtt cgaataccca tacgacgtcc cagactacgc ttagtaatga ttaattaaac
     5701 tagaaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagt aacccaccca
     5761 agatctggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga
     5821 gccgcgtaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg cccccctcct
     5881 cacggcgagc ctagtcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     5941 cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc
     6001 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     6061 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     6121 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     6181 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     6241 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     6301 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     6361 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     6421 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     6481 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     6541 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     6601 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     6661 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     6721 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     6781 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     6841 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     6901 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     6961 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     7021 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa
     7081 gcggccgcca tgctagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     7141 acggatccat aacttcgtat aatgtatgct atacgaagtt atgccaccat gtctagactg
     7201 gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg tatcgaaggc
     7261 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac
     7321 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat
     7381 acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag
     7441 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc
     7501 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc
     7561 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc
     7621 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat
     7681 tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg agccgaacct
     7741 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa
     7801 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc
     7861 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac
     7921 atgctccccg ggtaactaag taaggatccg cggccgcact agaggaattc cgcccctctc
     7981 cccccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     8041 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     8101 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     8161 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     8221 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     8281 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     8341 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     8401 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     8461 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     8521 ttttcctttg aaaaacacga tgataagctt accggtccac catggtgagc aagggcgagg
     8581 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     8641 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     8701 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     8761 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8821 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8881 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8941 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     9001 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     9061 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     9121 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     9181 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9241 ccgccgggat cactctcggc atggacgagc tgtacaagta agcatgcccg acggcgagga
     9301 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     9361 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     9421 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     9481 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     9541 cttctgatgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
     9601 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
     9661 tgaatgcaat tgttgttgtt tagtccctcc caattcgata tcaagcttat cgcgatagat
     9721 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     9781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     9841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     9901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     9961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
    10021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
    10081 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
    10141 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
    10201 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
    10261 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
    10321 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
    10381 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
    10441 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10501 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10561 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10621 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
    10681 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
    10741 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
    10801 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
    10861 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
    10921 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
    10981 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
    11041 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
    11101 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
    11161 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
    11221 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
    11281 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
    11341 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
    11401 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
    11461 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
    11521 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
    11581 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
    11641 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
    11701 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
    11761 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
    11821 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
    11881 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
    11941 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
    12001 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
    12061 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
    12121 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
    12181 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
    12241 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
    12301 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
    12361 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
    12421 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    12481 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    12541 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    12601 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    12661 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    12721 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    12781 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    12841 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
    12901 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
    12961 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
    13021 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
    13081 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
    13141 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
    13201 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
    13261 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
    13321 acaaacttag tagt
//
