LOCUS       sequence-33380-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt R3L2-MCS
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    168..267
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    337..460
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(475..498)
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     terminator      complement(506..533)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(625..711)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtggatttat cgcggcctcg acgtttcccg ttgaatatgg ctcataacac cccttgtatt
       61 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
      121 gtaacatcag agattttgag acacgggcca gagctgcagc tggatggcaa ataatgattt
      181 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa
      241 tgccaacttt gtacaagaaa gctgggtcta gatatctcga gtcatatgaa agtcctgagg
      301 ttaattaaga tagcggccgc attcagagat cttaagcaac tatgtataat aaagttgaac
      361 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
      421 ctacataata ctgtaaaaca caacatatcc agtcactatg gagatcttaa gtaactaact
      481 aacaggaaga gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttagt
      541 ttgatgcctg gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcac
      601 aacgttcaaa tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca
      661 acagataaaa cgaaaggccc agtcttccga ctgagccttt cgttttattt gatgcctggc
      721 agttccctac tctcgcttag tagttagacg tccccgagat ccatgctagc ggtaatacgg
      781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
      841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      901 gagctccaaa c
//
