LOCUS       sequence-333801-        7482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Olig001 encodes a novel AAV capsid that exhibits preferential 
            oligodendrocyte tropism in the CNS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Powell SK, Khan N, Parker CL, Samulski RJ, Matsushima G, Gray SJ, 
            McCown TJ
  TITLE     Characterization of a novel adeno-associated viral vector with 
            preferential oligodendrocyte tropism.
  JOURNAL   Gene Ther. 2016 Nov;23(11):807-814. doi: 10.1038/gt.2016.62. Epub 
            2016 Sep 15.
  PUBMED    27628693
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/gt.2016.62"; journalName: "Gene Ther"; date: "2016-11"; 
            volume: "23"; issue: "11"; pages: "807-814"
COMMENT     Sequence Label: Olig001
FEATURES             Location/Qualifiers
     source          1..7482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4114..4125
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(4367..4385)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4406..4422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4406..4422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4419..4441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4430..4446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4454..4460,4461..4478,4479..4484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4499..4520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4637..4654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4808..5396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4888..4907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5567..6358,6359..6427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6190..6209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6428..6532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6600..6618
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6656..6678)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6778..6797
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6864..7319)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7001..7022)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7213..7232
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7445..7467
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7459..7476
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7460..7476
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 taatacgact cactataggg cgaattcgag ctcggtaccc ctagaggtcc tgtattagag
       61 gtcacgtgag tgttttgcga cattttgcga caccatgtgg tcacgctggg tatttaagcc
      121 cgagtgagca cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccaccacgc
      181 cggggtttta cgagattgtg attaaggtcc ccagcgacct tgacgagcat ctgcccggca
      241 tttctgacag ctttgtgaac tgggtggccg agaaggaatg ggagttgccg ccagattctg
      301 acatggatct gaatctgatt gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg
      361 actttctgac ggaatggcgc cgtgtgagta aggccccgga ggcccttttc tttgtgcaat
      421 ttgagaaggg agagagctac ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat
      481 ccatggtttt gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag agaatttacc
      541 gcgggatcga gccgactttg ccaaactggt tcgcggtcac aaagaccaga aatggcgccg
      601 gaggcgggaa caaggtggtg gatgagtgct acatccccaa ttacttgctc cccaaaaccc
      661 agcctgagct ccagtgggcg tggactaata tggaacagta tttaagcgcc tgtttgaatc
      721 tcacggagcg taaacggttg gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc
      781 agaacaaaga gaatcagaat cccaattctg atgcgccggt gatcagatca aaaacttcag
      841 ccaggtacat ggagctggtc gggtggctcg tggacaaggg gattacctcg gagaagcagt
      901 ggatccagga ggaccaggcc tcatacatct ccttcaatgc ggcctccaac tcgcggtccc
      961 aaatcaaggc tgccttggac aatgcgggaa agattatgag cctgactaaa accgcccccg
     1021 actacctggt gggccagcag cccgtggagg acatttccag caatcggatt tataaaattt
     1081 tggaactaaa cgggtacgat ccccaatatg cggcttccgt ctttctggga tgggccacga
     1141 aaaagttcgg caagaggaac accatctggc tgtttgggcc tgcaactacc gggaagacca
     1201 acatcgcgga ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg
     1261 agaactttcc cttcaacgac tgtgtcgaca agatggtgat ctggtgggag gaggggaaga
     1321 tgaccgccaa ggtcgtggag tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg
     1381 accagaaatg caagtcctcg gcccagatag acccgactcc cgtgatcgtc acctccaaca
     1441 ccaacatgtg cgccgtgatt gacgggaact caacgacctt cgaacaccag cagccgttgc
     1501 aagaccggat gttcaaattt gaactcaccc gccgtctgga tcatgacttt gggaaggtca
     1561 ccaagcagga agtcaaagac tttttccggt gggcaaagga tcacgtggtt gaggtggagc
     1621 atgaattcta cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt gacgcagata
     1681 taagtgagcc caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag
     1741 cttcgatcaa ctacgcagac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc
     1801 tgatgctgtt tccctgcaga caatgcgaga gaatgaatca gaattcaaat atctgcttca
     1861 ctcacggaca gaaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg
     1921 tcgtcaaaaa ggcgtatcag aaactgtgct acattcatca tatcatggga aaggtgccag
     1981 acgcttgcac tgcctgcgat ctggtcaatg tggatttgga tgactgcatc tttgaacaat
     2041 aaatgattta aatcaggtat ggctgccgat ggttatcttc cagattggct cgaggacact
     2101 ctctctgaag gaataagaca gtggtggaag ctcaaacctg gcccaccacc accaaagccc
     2161 gcagagcggc ataaggacga cagcaggggt cttgtgcttc ctgggtacaa gtacctcgga
     2221 cccttcaacg gactcgacaa gggagagccg gtcaacgagg cagacgccgc ggccctcgag
     2281 cacgacaaag cctacgaccg gcagctcgac agcggagaca acccgtacct caagtacaac
     2341 cacgccgacg cggagtttca ggagcgcctt aaagaagata cgtcttttgg gggcaacctc
     2401 gggcgagcag tcttccaggc caaaaagagg cttcttgaac ctcttggtct ggttgaggaa
     2461 gcggctaaga cggctcctgg aaagaagagg cctgtagagc agtctcctca ggaaccggac
     2521 tcctcctcgg gcatcggcaa gacaggccag cagcccgcta aaaagagact caatttcggt
     2581 cagactggcg acacagagtc agtcccagac cctcaaccaa tcggagaacc tcccgcagcc
     2641 ccctcaggtg tgggatctct tacaatggct tcaggtggtg gcgcaccagt ggcagacaat
     2701 aacgaaggtg ccgatggagt gggtagttcc tcgggaaatt ggcattgcga ttcccaatgg
     2761 ctgggggaca gagtcatcac caccagcacc cgaacctggg ccctgcccac ctacaacaat
     2821 cacctctaca agcaaatctc caacgggaca tcgggaggag ccaccaacga caacacctac
     2881 ttcggctaca gcaccccctg ggggtatttt gactttaaca gattccactg ccacttttca
     2941 ccacgtgact ggcagcgact catcaacaac aactggggat tccggcccaa gagactcagc
     3001 ttcaagctct tcaacatcca ggtcaaggag gtcacgcaga atgaaggcac caagaccatc
     3061 gccaataacc ttaccagcac ggtccaggtc ttcacggact cggagtacca gctgccgtac
     3121 gttctcggct ctgcccacca gggctgcctg cctccgttcc cggcggacgt gttcatgatt
     3181 ccccagtacg gctacctaac actcaacaac ggtagtcagg ccgtgggacg ctcctccttc
     3241 tactgcctgg aatactttcc ttcgcagatg ctgagaaccg gcaacaactt ccagtttact
     3301 tacaccttcg aggacgtgcc tttccacagc agctacgccc acagccagag cttggaccgg
     3361 ctgatgaatc ctctgattga ccagtacctg tactacttgt ctcggactca aacaacagga
     3421 ggcacggcaa atacgcagac tctgggcttc agccaaggtg ggcctaatac aatggccaat
     3481 caggcaaaga actggctgcc aggaccctgt taccgccaac aacgcgtctc aacgacaacc
     3541 gggcaaaaca acaatagcaa ctttgcctgg actgctggga ccaaatacca tctgaatgga
     3601 agaaattcat tggctaatcc tggcatcgct atggcaacac acaaagacga caaggagcgt
     3661 ttttttccca gtaacgggat cctgattttt ggcaaacaaa atgctgccag agacaatgcg
     3721 gattacagcg atgtcatgct caccagcgag gaagaaatca aaaccactaa ccctgtggct
     3781 acagaggaat acggtatcgt ggcagataac ttgcagcagc aaaacacggc tcctcaaatt
     3841 ggaactgtca acagccaggg ggccttaccc ggtatggttt ggcagaaccg ggacgtgtac
     3901 ctgcagggtc ccatctgggc caagattcct cacacggacg gcaacttcca cccgtctccg
     3961 ctgatgggcg gctttggcct gaaacatcct ccgcctcaga tcctgatcaa gaacacgcct
     4021 gtacctgcgg atcctccgac caccttcaac cagtcaaagc tgaactcttt catcacgcaa
     4081 tacagcaccg gacaggtcag cgtggaaatt gaatgggagc tgcagaagga aaacagcaag
     4141 cgctggaacc ccgagatcca gtacacctcc aactactaca aatctacaag tgtggacttt
     4201 gctgttaata cagaaggcgt gtactctgaa ccccacccca ttggcacccg ttacctcacc
     4261 cgtcccctgt aattgtctgt taatcaataa accggttgat tcgtttcagt tgacttggtg
     4321 tctagagggc cgctcgatgc atcgatgcat cgataagctt ttgttccctt tagtgagggt
     4381 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4441 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     4501 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4561 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4621 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     4681 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     4741 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4801 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4861 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4921 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4981 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     5041 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     5101 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     5161 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     5221 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     5281 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     5341 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     5401 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     5461 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     5521 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5581 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5641 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5701 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5761 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5821 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5881 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5941 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     6001 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     6061 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     6121 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     6181 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     6241 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     6301 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6361 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6421 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6481 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     6541 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     6601 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     6661 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     6721 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     6781 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6841 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     6901 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     6961 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     7021 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     7081 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     7141 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     7201 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     7261 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     7321 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     7381 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     7441 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tg
//
