LOCUS       sequence-333807-        7545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Genetically encoded HomoFRET-based probe to monitor peroxiredoxin1 
            (Prx1) oligomerization (for stable cell line generation).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7545)
  AUTHORS   Pastor-Flores D, Talwar D, Pedre B, Dick TP
  TITLE     Real-time monitoring of peroxiredoxin oligomerization dynamics in 
            living cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 
            10.1073/pnas.1915275117.
  PUBMED    32601209
REFERENCE   2  (bases 1 to 7545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Jun 29. pii: 1915275117. doi: 
            10.1073/pnas.1915275117."
COMMENT     Sequence Label: pLPCX mCER-Prx1
FEATURES             Location/Qualifiers
     source          1..7545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             68..661
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(764..786)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     protein_bind    914..947
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1007..1040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     1060..1079
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1160..1177)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1331..1919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1411..1430)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2090..2881,2882..2950))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2713..2732
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2951..3055)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3123..3141
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             3743..3918
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    3981..4180
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4381..4797
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4727..4749
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4765..4781
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             4894..5493
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4894..4913)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5390..5410
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        5668..5971
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5972..6175
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6126..6146
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(6226..6228,6229..6231,6232..6942)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6271..6292)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6532..6551)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6879..6900
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taagcggccg cctcggccaa acatcgataa aataaaagat tttatttagt ctccagaaaa
       61 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
      121 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
      181 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
      241 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
      301 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
      361 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
      421 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
      481 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
      541 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
      601 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcat
      661 ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc accaccggga
      721 ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
      781 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
      841 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
      901 gcggagtgta ctgataactt cgtataatgt atgctatacg aagttattag gtctgaagag
      961 gagtttacgt ccagccaagc tagggccgcg atccggaacc cttaatataa cttcgtataa
     1021 tgtatgctat acgaagttat cagtactggc ttaactatgc ggcatcagag cagattgtac
     1081 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     1141 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1201 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1261 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1321 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     1381 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     1441 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     1501 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     1561 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1621 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     1681 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     1741 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     1801 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     1861 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     1921 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     1981 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2041 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2101 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2161 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2221 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2281 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     2341 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     2401 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     2461 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     2521 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     2581 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     2641 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     2701 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     2761 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     2821 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     2881 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     2941 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3001 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3061 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     3121 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt gctagcaatt gctagcaatt
     3181 gctagcaatt cataccagat caccgaaaac tgtcctccaa atgtgtcccc ctcacactcc
     3241 caaattcgcg ggcttctgcc tcttagacca ctctacccta ttccccacac tcaccggagc
     3301 caaagccgcg gcccttccgt ttctttgctt ttgaaagacc ccacccgtag gtggcaagct
     3361 agcttaagta acgccacttt gcaaggcatg gaaaaataca taactgagaa tagaaaagtt
     3421 cagatcaagg tcaggaacaa agaaacagct gaataccaaa caggatatct gtggtaagcg
     3481 gttcctgccc cggctcaggg ccaagaacag atgagacagc tgagtgatgg gccaaacagg
     3541 atatctgtgg taagcagttc ctgccccggc tcggggccaa gaacagatgg tccccagatg
     3601 cggtccagcc ctcagcagtt tctagtgaat catcagatgt ttccagggtg ccccaaggac
     3661 ctgaaaatga ccctgtacct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     3721 gcgcgcttcc gctctccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgccag
     3781 tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc ttgctgtttg
     3841 catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactaccca
     3901 cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc ccagggacca
     3961 ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc cgattgtcta
     4021 gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta gctctgtatc
     4081 tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc tgggagacgt
     4141 cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc gatgtggaat
     4201 ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct
     4261 ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct gctgcagcgc
     4321 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
     4381 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     4441 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     4501 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     4561 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     4621 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     4681 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     4741 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccgga
     4801 atgatccccc gggctgcagg tcggccgcca cgaccggtgc cgccaccatc ccctgaccca
     4861 cgcccctgac ccctcacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc
     4921 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac
     4981 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg
     5041 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     5101 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     5161 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     5221 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     5281 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     5341 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     5401 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     5461 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     5521 accgaaagga gcgcacgacc ccatggctcc gaccgaagcc acccggggcg gccccgccga
     5581 ccccgcaccc gcccccgagg cccaccgact ctagtaatag taatcaatta cggggtcatt
     5641 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     5701 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     5761 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     5821 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     5881 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     5941 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     6001 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     6061 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     6121 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
     6181 agtgaaccgt cagatccgct agcgctaccg gactcagatc tcgagatggt gagcaagggc
     6241 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     6301 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     6361 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     6421 acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     6481 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     6541 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     6601 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     6661 gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac
     6721 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     6781 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     6841 tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     6901 accgccgccg ggatcactct cggcatggac gagctgtaca agggtaccat gtcttcagga
     6961 aatgctaaaa ttgggcaccc tgcccccaac ttcaaagcca cagctgttat gccagatggt
     7021 cagtttaaag atatcagcct gtctgactac aaaggaaaat atgttgtgtt cttcttttac
     7081 cctcttgact tcacctttgt gtgccccacg gagatcattg ctttcagtga tagggcagaa
     7141 gaatttaaga aactcaactg ccaagtgatt ggtgcttctg tggattctca cttctgtcat
     7201 ctagcatggg tcaatacacc taagaaacaa ggaggactgg gacccatgaa cattcctttg
     7261 gtatcagacc cgaagcgcac cattgctcag gattatgggg tcttaaaggc tgatgaaggc
     7321 atctcgttca ggggcctttt tatcattgat gataagggta ttcttcggca gatcactgta
     7381 aatgacctcc ctgttggccg ctctgtggat gagactttga gactagttca ggccttccag
     7441 ttcactgaca aacatgggga agtgtgccca gctggctgga aacctggcag tgataccatc
     7501 aagcctgatg tccaaaagag caaagaatat ttctccaagc agaag
//
