LOCUS       sequence-333808-        4113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4113)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-HKU1 N_nostop
FEATURES             Location/Qualifiers
     source          1..4113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1456..1555)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1572..1591)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1573..1591)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1596..1612)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1596..1612)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2043..2834
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2927..3515
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3416..3435
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3669..3686
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3845..3872)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3964..4050)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4099..4113,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4113,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catgtcttat actcccggtc attatgctgg aagtagaagc tcctctggaa
      181 atcgttcagg aatcctcaag aaaacttctt gggctgacca atctgagcga aattaccaaa
      241 cctttaatag aggcagaaaa acccaaccta aattcactgt gtctactcaa ccacaaggaa
      301 atactatccc acattattcc tggttctccg ggatcactca atttcaaaaa ggtagagact
      361 ttaaattttc agatggtcaa ggagttccca ttgctttcgg agtaccccct tctgaagcaa
      421 aaggatattg gtatagacac agccggcgtt cttttaaaac agctgatggt caacaaaagc
      481 agttgttacc gagatggtat ttctactatc tcggtaccgg cccatatgcc aatgcatcct
      541 atggtgaatc cctcgaaggg gtcttctggg ttgctaatca ccaagctgac acttctactc
      601 cctccgatgt ttcgtcaagg gaccctacta ctcaagaagc tatccctact aggtttccgc
      661 ctggtacgat tttgcctcaa ggctattatg ttgaaggctc aggaaggtct gcttctaata
      721 gtcgaccagg ttcacgttct caatcacgtg gacccaataa tcgttcatta agtagaagta
      781 attctaattt tagacattca gattctatag taaaacctga tatggctgat gagatcgcta
      841 atcttgtttt agccaagctt ggtaaagatt ctaaacctca gcaagtcact aagcaaaatg
      901 ccaaggaaat caggcataaa attttaacaa aacctcgcca aaagcgaact cctaataaac
      961 attgtaatgt tcaacagtgt tttggtaaaa gaggaccttc tcaaaatttt ggtaatgctg
     1021 aaatgttaaa gcttggtact aatgatcctc agtttcctat tcttgcagaa ttagctccta
     1081 caccaggtgc ttttttcttt ggttctaaat tagacttggt taaaagagat tccgaggctg
     1141 actcacctgt taaagatgtt tttgaacttc attattctgg ttctattagg tttgatagta
     1201 ctttaccagg ctttgagaca attatgaaag ttcttgaaga gaatttaaat gcttacgtta
     1261 attctaatca gaacactgat tctgattcgt tgagttctaa acctcagcgt aaaagaggtg
     1321 ttaaacaatt accagaacag tttgactctc ttaatttaag tgctggtact cagcacattt
     1381 caaatgattt tactcctgag gatcatagtt tacttgctac tcttgatgat ccttatgtag
     1441 aagactctgt tgcttaccca actttcttgt acaaagttgg cattataaga aagcattgct
     1501 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1561 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1621 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1681 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1741 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1801 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1861 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1921 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1981 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     2041 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2101 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2161 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2221 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2281 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2341 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2401 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2461 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2521 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2581 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2641 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2701 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2761 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2821 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2881 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2941 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3001 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3061 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3121 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3181 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3241 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3301 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3361 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3421 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3481 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3541 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3601 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3661 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3721 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3781 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3841 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3901 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3961 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     4021 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     4081 cgctagcatg gatgttttcc cagtcacgac gtt
//
