LOCUS       sequence-33381-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt L1L3 EF1a-tTA-2
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     LTR             99..367
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(380..591)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tctatgcgct gttaatcact ttacttttat ctaatctaga catatgaatt ccccggatcc
       61 gtcgacctgc aggccttgta ggcgccggtc acagcttgga tctgtaacgg cgcagaacag
      121 aaaacgaaac aaagacgtag agttgagcaa gcagggtcag gcaaagcgtg gagagccggc
      181 tgagtctagg taggctccaa gggagcgccg gacaaaggcc cggtctcgac ctgagcttta
      241 aacttaccta gacggcggac gcagttcagg aggcaccaca ggcgggaggc ggcagaacgc
      301 gactcaaccg gcgtggatgg cggcctcagg tagggcggcg ggcgcgtgaa ggagagatgc
      361 gagcccctcg aagcttcagc tgtgttctgg cggcaaaccc gttgcgaaaa agaacgttca
      421 cggcgactac tgcacttata tacggttctc ccccaccctc gggaaaaagg cggagccagt
      481 acacgacatc actttcccag tttaccccgc gccaccttct ctaggcaccg gttcaattgc
      541 cgacccctcc ccccaacttc tcggggactg tgggcgatgt gcgctctgcc cactgacggg
      601 caccggagcg atcgcagatc ttaattaaca tatgtggatc cccgggtacc gagttggcgc
      661 gcctgggagc tcacggggac agcccccccc caaagccccc agggatgtaa ttacgtccct
      721 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
      781 gcatccccga gccggcagcg tgcggggaca gcccgggcac gggg
//
