LOCUS       sequence-333812-        4116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pDONR223 HCoV-HKU1 N_stop
FEATURES             Location/Qualifiers
     source          1..4116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    33..132
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1459..1558)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1575..1594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1576..1594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1599..1615)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1599..1615)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2046..2837
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2930..3518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3419..3438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3672..3689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3848..3875)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3967..4053)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     join(4102..4116,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(4116,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtctt aagctcgggc cccaaataat gattttattt tgactgatag
       61 tgacctgttc gttgcaacaa attgatgagc aatgcttttt tataatgcca actttgtaca
      121 aaaaagttgg catgtcttat actcccggtc attatgctgg aagtagaagc tcctctggaa
      181 atcgttcagg aatcctcaag aaaacttctt gggctgacca atctgagcga aattaccaaa
      241 cctttaatag aggcagaaaa acccaaccta aattcactgt gtctactcaa ccacaaggaa
      301 atactatccc acattattcc tggttctccg ggatcactca atttcaaaaa ggtagagact
      361 ttaaattttc agatggtcaa ggagttccca ttgctttcgg agtaccccct tctgaagcaa
      421 aaggatattg gtatagacac agccggcgtt cttttaaaac agctgatggt caacaaaagc
      481 agttgttacc gagatggtat ttctactatc tcggtaccgg cccatatgcc aatgcatcct
      541 atggtgaatc cctcgaaggg gtcttctggg ttgctaatca ccaagctgac acttctactc
      601 cctccgatgt ttcgtcaagg gaccctacta ctcaagaagc tatccctact aggtttccgc
      661 ctggtacgat tttgcctcaa ggctattatg ttgaaggctc aggaaggtct gcttctaata
      721 gtcgaccagg ttcacgttct caatcacgtg gacccaataa tcgttcatta agtagaagta
      781 attctaattt tagacattca gattctatag taaaacctga tatggctgat gagatcgcta
      841 atcttgtttt agccaagctt ggtaaagatt ctaaacctca gcaagtcact aagcaaaatg
      901 ccaaggaaat caggcataaa attttaacaa aacctcgcca aaagcgaact cctaataaac
      961 attgtaatgt tcaacagtgt tttggtaaaa gaggaccttc tcaaaatttt ggtaatgctg
     1021 aaatgttaaa gcttggtact aatgatcctc agtttcctat tcttgcagaa ttagctccta
     1081 caccaggtgc ttttttcttt ggttctaaat tagacttggt taaaagagat tccgaggctg
     1141 actcacctgt taaagatgtt tttgaacttc attattctgg ttctattagg tttgatagta
     1201 ctttaccagg ctttgagaca attatgaaag ttcttgaaga gaatttaaat gcttacgtta
     1261 attctaatca gaacactgat tctgattcgt tgagttctaa acctcagcgt aaaagaggtg
     1321 ttaaacaatt accagaacag tttgactctc ttaatttaag tgctggtact cagcacattt
     1381 caaatgattt tactcctgag gatcatagtt tacttgctac tcttgatgat ccttatgtag
     1441 aagactctgt tgcttaatac ccaactttct tgtacaaagt tggcattata agaaagcatt
     1501 gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     1561 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     1621 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     1681 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     1741 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     1801 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     1861 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     1921 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     1981 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     2041 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     2101 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     2161 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     2221 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     2281 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     2341 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     2401 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     2461 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     2521 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     2581 gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac agcgcagtaa
     2641 ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc ctgccggccc
     2701 agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa gaagatcgct
     2761 tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc gagatcacca
     2821 aggtagtcgg caaataaccc tcgagccacc catgaccaaa atcccttaac gtgagttacg
     2881 cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3061 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     3301 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     3361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     3421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     3481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     3541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3721 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     3781 aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc tagccaggaa gagtttgtag
     3841 aaacgcaaaa aggccatccg tcaggatggc cttctgctta gtttgatgcc tggcagttta
     3901 tggcgggcgt cctgcccgcc accctccggg ccgttgcttc acaacgttca aatccgctcc
     3961 cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc
     4021 ccagtcttcc gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcgt
     4081 taacgctagc atggatgttt tcccagtcac gacgtt
//
