LOCUS       sequence-333815-        6561 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for insect cell expression of Tetrahymena thermophila Q22YU3
            C-terminally tagged with 2x Strep-tag II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, 
            Rappsilber J, Skehel M, Carter AP
  TITLE     Shulin packages axonemal outer dynein arms for ciliary targeting.
  JOURNAL   Science. 2021 Feb 26;371(6532):910-916. doi: 
            10.1126/science.abe0526.
  PUBMED    33632841
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2021-02-26- 26"; volume: "371"; 
            issue: "6532"; pages: "910-916"
COMMENT     Sequence Label: pACEBac1-Q22YU3-2xStrep
FEATURES             Location/Qualifiers
     source          1..6561
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..99)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        176..267
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     192..209
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             3916..3939
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3940..3963
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4000..4023
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     polyA_signal    4183..4317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4220..4239)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4274..4293
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  4438..4603
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4793..5381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5282..5301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  5516..5739
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(5806..6339)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(6528..6533,6534..6550,6551..6556))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 ttcgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
       61 tcgccataac ttcgtatagc atacattata cgaagttatc tgtaactata acggtcctaa
      121 ggtagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt aatagatcat
      181 ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg ttttcgtaac
      241 agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc caccatcggg
      301 cgcgatccct cgagcatgtt taacttcttc tcctctgcta acatcaatca gaacatccca
      361 aagtattccg taaatgactt cgtgttccgc ctgaagaaga tcgagaagat cgtggtcaag
      421 gaaggattgg acggtttctt gctgatcaat ggcgtagatt cacgcgagaa cacagagtac
      481 gttaaactca cgaattggct gttcctgggt aactctggcc tggagatcga ggagaatgag
      541 tacctgaacc aaatctacag cgacatgatt gtgttgatta agaagggaac cacccacatc
      601 ttcattgacc ctgaggctct caattccttg caaaccctga tctactcaat tcccaacgtg
      661 gatgtcttct gtcccaccga gaagcaatac gaggacaagg atgaaatgga actcttgaag
      721 atggccttct tcctgagagt aatgaagcca accaagaagg tgggcatcct gttgggccag
      781 aaggataagg gtaagatcaa cagcatcgag aagtggcctt tgattcaaag ctacggtctg
      841 gaagaactcg gtgtgggctt cttctcaatg aatcatgagg ttgtggatct gacactgcgt
      901 ctgaacgcgg tgtacaagaa ctatgacaaa ttctttgttt ctaaactcat ctacgtagtt
      961 gccaagagac tgactggtca ctttaactca gccgctggtc aactcggtga catgaagatg
     1021 cataaacgca acttggccac ggaatcacaa ctcacggaaa tctttcgtga cacttatgag
     1081 attgaggaga ttagcaaatg ggtccaaatt cgcggtgtga atgcagcgtt gcccaaaccc
     1141 agagtgttgt tcggcaagaa cacgtcagct gattgctcaa aggagccctc tgtagcacca
     1201 ctgaaggatc tcaagtattc tgaaacgttc cactctttcc acgctacatt cgagacattt
     1261 gatctcagga catgtctcag ggcagctcgc acctacttcc tggctaaagg tgttaaagag
     1321 gagagaaact tgatcacact gaatgacgac gagggagtcc ctcaaggtta tgagttgaac
     1381 attgatgaga accaacagta caaggatcag gacttcctcg ctaacttgta cttgagcatc
     1441 atcattggct ttaacgaggt tatgcagttg attaccaaag actacaagaa catgaccgag
     1501 gagttcatcc aggattacat cttccagaag gtctccaaag tgtatgctgg cttccaaatc
     1561 cctgaatcag aaatcacatt ggacaagatc caaatcattc tgaaagcgta caattcattc
     1621 ggtgaagagg tgaagattga cttcaaagac actatctcat tcaagttgac accatacttc
     1681 ttcatggttc gtatcgaaca gaagaacatc aagagccaaa tcctgaacaa tacggtattg
     1741 ggttctctcg tgttcgctga gtctttcatt ctccaggagg gatgctacct gctgctcact
     1801 aaagagatcc catacttcga tctgtggaac tgtcagaacg actactcaga gaagatcgag
     1861 aagatgaaga agcgtatctt gtgggaacca ctgggcaaac aaatctccga cgaactgccc
     1921 aagaacagga tctttgtcca gactggcaga aagagcaatt acggctttga cattcccatt
     1981 atgcaggctt cctattacat gcacgaactc ggtctgcgca tcgaaactca acgcctgggc
     2041 tggttcatct tgttcttcaa ggagatgaag gagatccaaa tcacacagaa gatgaatcac
     2101 acctggctga tcttcaaagt ggattccaac atcaccttta actcaattag caaggacaca
     2161 atcgcgttgg agttcactgg agatgccttg gagcagagct tctttaagat caagaattac
     2221 ttcgaggaga atcaaatcaa atacgagtac caagtagaca tcccagcaat cttccaggag
     2281 tctcagatcg ccaagaaaca aatcctcaat cagcaatcac aaggtcagaa actcattacc
     2341 atgaactcaa tccagaatga acagttcttc atctcttaca ttgaaagcaa acagctcatg
     2401 attctcaacc agatgaagga cttgaaattg tccgtctaca agaacctgta cgaacaaatg
     2461 cagatttctc aggctatcac tcccgttgag aaccacatcg gtgtcatcct ggttaacggt
     2521 agctactgct ccggtaagcg taagttcgct gagaacctca tccgcttcgg cagcgacaac
     2581 aatctccgcc tgcacctgta caaattcgat ctgaacgaga tgtcagaact cacagagaag
     2641 tcctacttga gcggactgct caaattcgcg agcgagaaga agatccagaa cacggacgtt
     2701 attgtggctt ccgtccctca ctttatcaac acgaagattt tgatcgacta tttcagcaag
     2761 tcagagaaga tttcaaatgc cttctacatt cgcacaatcg cgaccaagat caacatcaac
     2821 aacatctaca gcaactttaa caagaatccc gtcaacaacg tgttcactta tggtgtggaa
     2881 ggctactccc aattcctcct cctggatacc tacaacaact acgatgcaga cgtgaacgct
     2941 ctgaacaaga cgctctccgg agtcctccct ggtgccaaaa tttacaagat tatgaacaat
     3001 atcctgaatc ccgcattggc aaaggatatt ctgacgtcca ttaccttcat cagcgaacag
     3061 aacaatttga accgtctgaa gtactcagtg caatacgacc tgctcacttc caatggtcct
     3121 tcttcagtcg tgttcattcc ctttaagttg ccaattctga gggagaagat ccgtgacctc
     3181 atctataaga agattttgca gaacggaaac cagactttgg tggacacgat tgaagccgag
     3241 cagaagatcg ccgagttcaa ggagctgaat aagaactcta aagacccttt gatgatcgag
     3301 atcattaagc tgaaggagaa gatcgagatc cagaacgcac agacttccga ccaagccatc
     3361 aagatcgatt atgttaaagg tatcctgcgc tacgactcca agctgaagga aggtctcgaa
     3421 gagattacta tcacaccaaa ctacttcatt gaacgcactg tgaagggtgt tgacgcaaag
     3481 gagtttactg aagaactcaa cggagtctca ttcaagaatg tgaagtacac aggaatcact
     3541 aattccatca ttaacgatat gggattcgtg ttcgctggca agaacctgaa caaagagaag
     3601 ttgctggagc tgctgtacaa attggtcaag ccactgaaca aacagaagct gcgccagagg
     3661 aaggacttga ctgaagagga gattgtagac atccagtttc gtaaccgtgg tgaaggactg
     3721 gagaacggcg agttctacga tggacaattc tggcgcaata tccagggtct gattctccct
     3781 caccatccca agaaggacga gttcatcgag gaatacctca agcaagagga agtcaggatc
     3841 aaccagatta acgagcagct gcaacaagaa tgggaaactt ggaagcaagt gtacgacaag
     3901 attcacctgg acaagctgga agttctgttt cagggtccgt ggtcacatcc gcagtttgaa
     3961 aaaggtagcg caggtagtgc agcaggtagc ggtgcaggtt ggagccatcc tcagtttgag
     4021 aaataaggta cctctagagc ctgcagtctc gacaagcttg tcgagaagta ctagaggatc
     4081 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4141 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4201 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4261 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga
     4321 tcactgcttg agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     4381 tgctgccacc gctgagcaat aactatcata acccctaggg tatacccatc taattggaac
     4441 cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt attagcttac
     4501 gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc ttaaaaactc
     4561 catttccacc cctcccagtt cccaactatt ttgtccgccc acaaccggtt gacttgggtc
     4621 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     4681 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     4741 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     4801 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     4861 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     4921 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     4981 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     5041 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     5101 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     5161 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     5221 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     5281 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     5341 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     5401 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     5461 ctgattgact tgggtcgctc ttcctgtgga tgcgcagatg ccctgcgtaa gcgggtgtgg
     5521 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa
     5581 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg
     5641 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt
     5701 aaagaggggc gtggccaagg gcatgtaaag actatattcg cggcgttgtg acaatttacc
     5761 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     5821 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     5881 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     5941 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     6001 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     6061 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     6121 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     6181 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     6241 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     6301 actgccctgc tgcgtaacat cgttgctgct gcgtaacatc gttgctgctc cataacatca
     6361 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtacaa
     6421 aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg
     6481 tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt ttacgaaccg
     6541 aacaggctta tgtcaactgg g
//
