LOCUS       sequence-33382-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt L1L3 rtTA-3
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(128..161)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagc aggctggcgc cggaaccaat tcagtcgacg
      121 tttaaacata acttcgtata gcatacatta tacgaagtta tctcgagcct caggaaacag
      181 ggtacccggg gatccacata tgttaattaa gactcctgca ggattaagcc cgggcgaatt
      241 caccatgtct agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg
      301 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
      361 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
      421 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
      481 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt
      541 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
      601 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
      661 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
      721 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
      781 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
      841 acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat
      901 gctcccagcc gatgccc
//
