LOCUS       sequence-33383-e         503 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 503)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt L1L3 rtTA-3
FEATURES             Location/Qualifiers
     source          1..503
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      222..308
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      401..428
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     436..459
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgctagcat ggatctcggg
      181 gacgtctaac tactaagcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
      241 agtcggaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
      301 ggacaaatcc gccgggagcg gatttgaacg ttgtgaagca acggcccgga ggggtggcgg
      361 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      421 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      481 ataatgattt tattttgact gat
//
