LOCUS       sequence-33384-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt R3L2 TetO(fl)-3
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    159..258
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    280..313
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(336..354,355..377,378..396,397..419,420..438,439..461,
                     462..480,481..503,504..522,523..545,546..564,565..587,
                     588..606)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        639..677
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     674..698
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tcgcggcctc gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
       61 gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca
      121 gagattttga gacacgggcc agagctgcag ctggatggca aataatgatt ttattttgac
      181 tgatagtgac ctgttcgttg caacaaattg ataagcaatg ctttcttata atgccaactt
      241 tgtacaagaa agctgggtct agatatctcg agtttaaaca taacttcgta taatgtatgc
      301 tatacgaagt tatcataggt tatcgagttt accactccct atcagtgata gagaaaagtg
      361 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
      421 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
      481 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
      541 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
      601 tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg
      661 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
      721 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcgagc
      781 tcggtacccg gggatcctga gaacttc
//
