LOCUS       sequence-33385-e         879 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
  TITLE     Constitutive Gs activation using a single-construct 
            tetracycline-inducible expression system in embryonic stem cells and
            mice.
  JOURNAL   Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
  PUBMED    21375737
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res Ther. 2011 Mar 4. 2(2):11."
COMMENT     Sequence Label: pEnt R3L2 TetO(fl)-3
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      216..302
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      394..421
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     429..452
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    complement(467..590)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    617..665
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 actgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
       61 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      121 gcgttatccc ctgattctgt ggataaccgt attaccgcta gcatggatct cggggacgtc
      181 taactactaa gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg
      241 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa
      301 atccgccggg agcggatttg aacgttgtga agcaacggcc cggagggtgg cgggcaggac
      361 gcccgccata aactgccagg catcaaacta agcagaaggc catcctgacg gatggccttt
      421 ttgcgtttct acaaactctt cctgttagtt agttacttaa gatctccata gtgactggat
      481 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
      541 gatatttata tcattttacg tttctcgttc aactttatta tacatagttg cttaagatct
      601 ctgaatgcgg ccgcttcaca caaaaaacca acacacagat gtaatgaaaa taaagatatt
      661 ttattgcggc catcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
      721 agtcccgcct gcaggccggc ctagtgcagg gcgtcgacga attctttgcc aaaatgatga
      781 gacagcacaa caaccagcac gttgcccagg agctgtagga aagagaagaa ggcatgaaca
      841 tggttagcag aggggccccg gtttggactc agagtattt
//
