LOCUS       sequence-333867-       13216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RFP cDNA and SAE1 shRNA 3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13216)
  AUTHORS   Yu B, Swatkoski S, Holly A, Lee LC, Giroux V, Lee CS, Hsu D, Smith 
            JL, Yuen G, Yue J, Ann DK, Simpson RM, Creighton CJ, Figg WD, Gucek 
            M, Luo J
  TITLE     Oncogenesis driven by the Ras/Raf pathway requires the SUMO E2 
            ligase Ubc9.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1724-33. doi: 
            10.1073/pnas.1415569112. Epub 2015 Mar 24.
  PUBMED    25805818
REFERENCE   2  (bases 1 to 13216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1415569112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "E1724-33"
COMMENT     Sequence Label: pInducer10-SAE1_sh3
FEATURES             Location/Qualifiers
     source          1..13216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2379..3074
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           3125..3246
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3274..3288
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3316..3442
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        3505..4716
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4553..4572
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4723..4756
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4763..5380,5381..5386,5387..5422,5423..5425,5426..5461,
                     5462..5464,5465..5500,5501..5509)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5558..6130
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5725..5742)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5952..5971
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6133..6735
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6632..6652
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6757..7345
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6810..6830)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7228..7239)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7552..7785
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7886..7903)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7892..8116
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8162..8590
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8249..8268)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8459..8480
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8599..8619)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8604..8933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8784..8919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8846..8865
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9756..9877
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9926..9942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9926..9942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9939..9961)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9950..9966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9974..9980,9981..9998,9999..10004))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10019..10040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10157..10174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10328..10916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10408..10427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11087..11878,11879..11947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11710..11729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11948..12052)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12100..12234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12402..13035
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13082..13207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tcgccaccat gagcgagctg atcaaggaga
     2401 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     2461 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
     2521 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaag
     2581 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     2641 tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     2701 ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg aacttcccat
     2761 ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc gagatgctgt
     2821 accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc gtgggcgggg
     2881 gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct aagaacctca
     2941 agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag gccgacaaag
     3001 agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc cctagcaaac
     3061 tggggcacag atgatgcggc cgcaagcctt gttaagtgct cgcttcggca gcacatatac
     3121 tatgttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt
     3181 gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata ttgctgttga
     3241 cagtgagcga tccagggatg tcatagttaa atagtgaagc cacagatgta tttaactatg
     3301 acatccctgg agtgcctact gcctcggaat tcaaggggct actttaggag caattatctt
     3361 gtttactaaa actgaatacc ttgctatctc tttgatacat ttttacaaag ctgaattaaa
     3421 atggtataaa ttaaatcact tttttcaatt ggaagactaa tgcggccggc cgtttaaaca
     3481 tttatatatg gacgcgtaag atctggcctc cgcgccgggt tttggcgcct cccgcgggcg
     3541 cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag cgtcctgatc
     3601 cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc ttagaacccc
     3661 agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca ctggttttct
     3721 ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct gcggagggat
     3781 ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg gcacagctag
     3841 ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt gatcgtcact
     3901 tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc cgctcggtgg
     3961 gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag caaggttgcc
     4021 ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt cttgaatgga
     4081 agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat ggtgggcggc
     4141 aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg ggtgagatgg
     4201 gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag aactcggttt
     4261 gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac ccgtaccttt
     4321 gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta taatgcaggg
     4381 tggggccacc tgccggtagg tgtgtggtag gcttttctcc gtcgcaggac gcagggttcg
     4441 ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg ggagggataa
     4501 gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta gctgaagctc
     4561 cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt ttaggcacct
     4621 tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag taaattgtcc
     4681 gctaaattct ggccgttttt ggcttttttg ttagacggat ccataacttc gtataatgta
     4741 tgctatacga agttatgcca ccatgtctag actggacaag agcaaagtca taaacggagc
     4801 tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa
     4861 gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg ccctgctcga
     4921 tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc tggaaggcga
     4981 gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc tcctctcaca
     5041 tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt acgaaaccct
     5101 ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg cactgtacgc
     5161 tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg agcatcaagt
     5221 agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc tgagacaagc
     5281 aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc tggaactaat
     5341 catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg acgcccttga
     5401 cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc ttgatatgct
     5461 gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaaa taacttcgta
     5521 tagcatacat tatacgaagt atggcgcgcc aattccgccc ctctccctcc ccccccccta
     5581 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     5641 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     5701 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     5761 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     5821 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     5881 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     5941 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     6001 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     6061 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6121 cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     6181 gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     6241 accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     6301 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     6361 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg ctcgcgcatg
     6421 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     6481 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     6541 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgctggg
     6601 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     6661 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     6721 aagcccggtg cctgattaat taagcgcgtc tggaacaatc aacctctgga ttacaaaatt
     6781 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     6841 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     6901 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     6961 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7021 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7081 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7141 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     7201 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7261 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7321 atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac ctagaaaaac
     7381 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7441 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7501 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga ctggaagggc
     7561 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     7621 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     7681 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     7741 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     7801 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggcctt
     7861 gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     7921 gtttgcccct tccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     7981 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8041 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8101 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8161 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8221 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8281 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8341 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8401 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8461 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8521 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8581 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8641 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8701 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8761 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8821 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     8881 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     8941 cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag cctgaactca
     9001 ccgcgacgtg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     9061 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     9121 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     9181 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     9241 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     9301 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     9361 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     9421 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     9481 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     9541 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     9601 gcactcgtcc gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct
     9661 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     9721 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     9781 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     9841 ctagttgtgg tttgtccaaa ctaatcaatg tatcttatca tgtctgtata ccgtcgacct
     9901 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     9961 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    10021 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    10081 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    10141 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    10201 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    10261 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    10321 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    10381 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    10441 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    10501 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    10561 ttcgctccaa gctgggctgt gtgcactaac cccccgttca gcccgaccgc tgcgccttat
    10621 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    10681 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    10741 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    10801 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    10861 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    10921 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    10981 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    11041 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    11101 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    11161 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    11221 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    11281 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    11341 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    11401 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    11461 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    11521 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    11581 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    11641 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    11701 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    11761 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    11821 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    11881 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    11941 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    12001 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    12061 cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat tgcagcttat
    12121 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    12181 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg
    12241 ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc
    12301 aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
    12361 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
    12421 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
    12481 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
    12541 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
    12601 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
    12661 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
    12721 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
    12781 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
    12841 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    12901 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    12961 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    13021 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
    13081 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
    13141 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
    13201 gagcgtcagt attaag
//
