LOCUS       sequence-333868-       13286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RFP cDNA and UBC9 shRNA 1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Yu B, Swatkoski S, Holly A, Lee LC, Giroux V, Lee CS, Hsu D, Smith 
            JL, Yuen G, Yue J, Ann DK, Simpson RM, Creighton CJ, Figg WD, Gucek 
            M, Luo J
  TITLE     Oncogenesis driven by the Ras/Raf pathway requires the SUMO E2 
            ligase Ubc9.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1724-33. doi: 
            10.1073/pnas.1415569112. Epub 2015 Mar 24.
  PUBMED    25805818
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1415569112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "E1724-33"
COMMENT     Sequence Label: pInducer10-UBC9-sh1
FEATURES             Location/Qualifiers
     source          1..13286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2379..3074
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           3125..3246
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3274..3288
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3316..3442
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(3537..3555)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3575..4786
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4623..4642
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4793..4826
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4833..5450,5451..5456,5457..5492,5493..5495,5496..5531,
                     5532..5534,5535..5570,5571..5579)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5628..6200
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5795..5812)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6022..6041
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6203..6805
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6702..6722
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6827..7415
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6880..6900)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7298..7309)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7622..7855
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7956..7973)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7962..8186
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8232..8660
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8319..8338)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8529..8550
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8669..8689)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8674..9003
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8854..8989
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8916..8935
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9826..9947
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9996..10012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9996..10012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10009..10031)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10020..10036
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10044..10050,10051..10068,10069..10074))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10089..10110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10398..10986)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10478..10497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11157..11948,11949..12017))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11780..11799
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12018..12122)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12170..12304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12472..13105
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13152..13277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tcgccaccat gagcgagctg atcaaggaga
     2401 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     2461 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
     2521 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaag
     2581 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     2641 tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     2701 ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg aacttcccat
     2761 ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc gagatgctgt
     2821 accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc gtgggcgggg
     2881 gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct aagaacctca
     2941 agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag gccgacaaag
     3001 agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc cctagcaaac
     3061 tggggcacag atgatgcggc cgcaagcctt gttaagtgct cgcttcggca gcacatatac
     3121 tatgttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt
     3181 gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata ttgctgttga
     3241 cagtgagcga gcctacacga tttactgcca atagtgaagc cacagatgta ttggcagtaa
     3301 atcgtgtagg cctgcctact gcctcggaat tcaaggggct actttaggag caattatctt
     3361 gtttactaaa actgaatacc ttgctatctc tttgatacat ttttacaaag ctgaattaaa
     3421 atggtataaa ttaaatcact tttttcaatt ggaagactaa tgcggccggc cagttcctct
     3481 ggaaagttaa aattttccct cttctgtggt taagcctcag gtcgtatcgt ttgggcccta
     3541 tagtgagtcg tattacctag gacgcgtaag atctggcctc cgcgccgggt tttggcgcct
     3601 cccgcgggcg cccccctcct cacggcgagc gctgccacgt cagacgaagg gcgcagcgag
     3661 cgtcctgatc cttccgcccg gacgctcagg acagcggccc gctgctcata agactcggcc
     3721 ttagaacccc agtatcagca gaaggacatt ttaggacggg acttgggtga ctctagggca
     3781 ctggttttct ttccagagag cggaacaggc gaggaaaagt agtcccttct cggcgattct
     3841 gcggagggat ctccgtgggg cggtgaacgc cgatgattat ataaggacgc gccgggtgtg
     3901 gcacagctag ttccgtcgca gccgggattt gggtcgcggt tcttgtttgt ggatcgctgt
     3961 gatcgtcact tggtgagtag cgggctgctg ggctggccgg ggctttcgtg gccgccgggc
     4021 cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg ctgtagtctg ggtccgcgag
     4081 caaggttgcc ctgaactggg ggttgggggg agcgcagcaa aatggcggct gttcccgagt
     4141 cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt tgaaacaagg tggggggcat
     4201 ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa tgcgggaaag ctcttattcg
     4261 ggtgagatgg gctggggcac catctgggga ccctgacgtg aagtttgtca ctgactggag
     4321 aactcggttt gtcgtctgtt gcgggggcgg cagttatggc ggtgccgttg ggcagtgcac
     4381 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta
     4441 taatgcaggg tggggccacc tgccggtagg tgtgtggtag gcttttctcc gtcgcaggac
     4501 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg
     4561 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta
     4621 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt
     4681 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag
     4741 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagacggat ccataacttc
     4801 gtataatgta tgctatacga agttatgcca ccatgtctag actggacaag agcaaagtca
     4861 taaacggagc tctggaatta ctcaatggtg tcggtatcga aggcctgacg acaaggaaac
     4921 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     4981 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     5041 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac cgctgtgctc
     5101 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     5161 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     5221 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     5281 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     5341 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     5401 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccgaccg
     5461 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     5521 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaaa
     5581 taacttcgta tagcatacat tatacgaagt atggcgcgcc aattccgccc ctctccctcc
     5641 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     5701 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     5761 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     5821 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     5881 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     5941 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     6001 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     6061 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     6121 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     6181 ctttgaaaaa cacgataata ccatggccac cgagtacaag cccacggtgc gcctcgccac
     6241 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     6301 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     6361 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     6421 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     6481 ctcgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     6541 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     6601 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     6661 gcgcgctggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     6721 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     6781 catgacccgc aagcccggtg cctgattaat taagcgcgtc tggaacaatc aacctctgga
     6841 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     6901 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     6961 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7021 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7081 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7141 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7201 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7261 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7321 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     7381 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attaattctg cagtcgagac
     7441 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     7501 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     7561 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagagggga
     7621 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7681 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7741 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7801 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7861 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7921 gagaggcctt gacattgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     7981 gccatctgtt gtttgcccct tccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     8041 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     8101 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     8161 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     8221 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8641 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     8701 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     8761 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     8821 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     8881 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     8941 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9001 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gatgaaaaag
     9061 cctgaactca ccgcgacgtg accgcaagga atcggtcaat acactacatg gcgtgatttc
     9121 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     9181 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     9241 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     9301 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     9361 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     9421 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     9481 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     9541 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     9601 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     9661 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tgctacgaga tttcgattcc
     9721 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     9781 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     9841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     9901 tcactgcatt ctagttgtgg tttgtccaaa ctaatcaatg tatcttatca tgtctgtata
     9961 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
    10021 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
    10081 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
    10141 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    10201 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    10261 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    10321 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    10381 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    10441 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    10501 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    10561 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    10621 gtgtaggtcg ttcgctccaa gctgggctgt gtgcactaac cccccgttca gcccgaccgc
    10681 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    10741 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    10801 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    10861 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    10921 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    10981 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    11041 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    11101 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    11161 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    11221 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    11281 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    11341 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    11401 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    11461 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    11521 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    11581 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    11641 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    11701 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    11761 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    11821 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    11881 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    11941 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    12001 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    12061 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    12121 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
    12181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
    12241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
    12301 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
    12361 taccactgcc aattacctgt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12421 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc
    12481 taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    12541 acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg
    12601 gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag
    12661 agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    12721 tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc
    12781 cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact
    12841 ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat
    12901 aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    12961 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    13021 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    13081 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    13141 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    13201 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    13261 atgggtgcga gagcgtcagt attaag
//
