LOCUS       sequence-333869-       13285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RFP cDNA and UBC9 shRNA 3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13285)
  AUTHORS   Yu B, Swatkoski S, Holly A, Lee LC, Giroux V, Lee CS, Hsu D, Smith 
            JL, Yuen G, Yue J, Ann DK, Simpson RM, Creighton CJ, Figg WD, Gucek 
            M, Luo J
  TITLE     Oncogenesis driven by the Ras/Raf pathway requires the SUMO E2 
            ligase Ubc9.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1724-33. doi: 
            10.1073/pnas.1415569112. Epub 2015 Mar 24.
  PUBMED    25805818
REFERENCE   2  (bases 1 to 13285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1415569112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "E1724-33"
COMMENT     Sequence Label: pInducer10-UBC9-sh3
FEATURES             Location/Qualifiers
     source          1..13285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    488..721
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             906..950
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1248..1365
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1430..1804)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1801..1810
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1814..1832)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1824..1871)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1941..1959,1960..1982,1983..2001,2002..2024,2025..2043,
                     2044..2066,2067..2085,2086..2108,2109..2127,2128..2150,
                     2151..2169,2170..2192,2193..2211)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2244..2282
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2303
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2379..3074
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           3125..3246
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3273..3287
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3315..3441
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        3574..4785
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4622..4641
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4792..4825
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4832..5449,5450..5455,5456..5491,5492..5494,5495..5530,
                     5531..5533,5534..5569,5570..5578)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5627..6199
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5794..5811)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6021..6040
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6202..6804
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6701..6721
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6826..7414
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6879..6899)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7297..7308)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7621..7854
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7955..7972)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7961..8185
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8231..8659
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8318..8337)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8528..8549
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8668..8688)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8673..9002
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8853..8988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8915..8934
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9825..9946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9995..10011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9995..10011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10008..10030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10019..10035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10043..10049,10050..10067,10068..10073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10088..10109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10226..10243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10397..10985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10477..10496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11156..11947,11948..12016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11779..11798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12017..12121)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12169..12303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12471..13104
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13151..13276
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
       61 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
      121 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
      181 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
      241 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
      301 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
      361 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
      421 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
      481 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
      541 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
      601 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
      661 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
      721 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
      781 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
      841 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
      901 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
      961 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     1021 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     1081 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     1141 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg
     1201 cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa
     1261 ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata
     1321 caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg
     1381 gacagcagag atccagtttg gttagtaccg ggcccgctct agtccggaat cagtcctgct
     1441 cctcggccac gaagtgcacg cagttgccgg ccgggtcgcg cagggcgaac tcccgccccc
     1501 acggctgctc gccgatctcg gtcatggccg gcccggaggc gtcccggaag ttcgtggaca
     1561 cgacctccga ccactcggcg tacagctcgt ccaggccgcg cacccacacc caggccaggg
     1621 tgttgtccgg caccacctgg tcctggaccg cgctgatgaa cagggtcacg tcgtcccgga
     1681 ccacaccggc gaagtcgtcc tccacgaagt cccgggagaa cccgagccgg tcggtccaga
     1741 actcgaccgc tccggcgacg tcgcgcgcgg tgagcaccgg aacggcactg gtcaacttgg
     1801 ccatggtggc cctcctatag tgagtcgtat tatactatgc cgatatacta tgccgatgat
     1861 taattgtcaa cacgtgctgc aggtccgagg ttctaggcta gcttaaagga accaattcag
     1921 tcgactggat cctttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     1981 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2221 gtcgagctcg gtacccgggt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag
     2281 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     2341 gaagacaccg ggaccgatcc agcctaccgg tcgccaccat gagcgagctg atcaaggaga
     2401 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     2461 ccgagggcga aggcaagccc tacgagggca cccagaccat gaagatcaag gtggtcgagg
     2521 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaag
     2581 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     2641 tcacatggga gagaatcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     2701 ccagcttcca gaacggctgc atcatctaca acgtcaagat caacggggtg aacttcccat
     2761 ccaacggccc tgtgatgcag aagaaaacac gcggctggga ggccaacacc gagatgctgt
     2821 accccgctga cggcggcctg agaggccaca gccagatggc cctgaagctc gtgggcgggg
     2881 gctacctgca ctgctccttc aagaccacat acagatccaa gaaacccgct aagaacctca
     2941 agatgcccgg cttccacttc gtggaccaca gactggaaag aatcaaggag gccgacaaag
     3001 agacctacgt cgagcagcac gagatggctg tggccaagta ctgcgacctc cctagcaaac
     3061 tggggcacag atgatgcggc cgcaagcctt gttaagtgct cgcttcggca gcacatatac
     3121 tatgttgaat gaggcttcag tactttacag aatcgttgcc tgcacatctt ggaaacactt
     3181 gctgggatta cttcttcagg ttaacccaac agaaggctcg agaaggtata ttgctgttga
     3241 cagtgagcgg gaacttctaa atgaaccaaa tagtgaagcc acagatgtat ttggttcatt
     3301 tagaagttcc ttgcctactg cctcggaatt caaggggcta ctttaggagc aattatcttg
     3361 tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa
     3421 tggtataaat taaatcactt ttttcaattg gaagactaat gcggccggcc agggtcgtgc
     3481 tattttcata taaggcggga aaccttgttt taagggagtt tagggttaat agggccctat
     3541 agtaagtcgt attacctagg acgcgtaaga tctggcctcc gcgccgggtt ttggcgcctc
     3601 ccgcgggcgc ccccctcctc acggcgagcg ctgccacgtc agacgaaggg cgcagcgagc
     3661 gtcctgatcc ttccgcccgg acgctcagga cagcggcccg ctgctcataa gactcggcct
     3721 tagaacccca gtatcagcag aaggacattt taggacggga cttgggtgac tctagggcac
     3781 tggttttctt tccagagagc ggaacaggcg aggaaaagta gtcccttctc ggcgattctg
     3841 cggagggatc tccgtggggc ggtgaacgcc gatgattata taaggacgcg ccgggtgtgg
     3901 cacagctagt tccgtcgcag ccgggatttg ggtcgcggtt cttgtttgtg gatcgctgtg
     3961 atcgtcactt ggtgagtagc gggctgctgg gctggccggg gctttcgtgg ccgccgggcc
     4021 gctcggtggg acggaagcgt gtggagagac cgccaagggc tgtagtctgg gtccgcgagc
     4081 aaggttgccc tgaactgggg gttgggggga gcgcagcaaa atggcggctg ttcccgagtc
     4141 ttgaatggaa gacgcttgtg aggcgggctg tgaggtcgtt gaaacaaggt ggggggcatg
     4201 gtgggcggca agaacccaag gtcttgaggc cttcgctaat gcgggaaagc tcttattcgg
     4261 gtgagatggg ctggggcacc atctggggac cctgacgtga agtttgtcac tgactggaga
     4321 actcggtttg tcgtctgttg cgggggcggc agttatggcg gtgccgttgg gcagtgcacc
     4381 cgtacctttg ggagcgcgcg ccctcgtcgt gtcgtgacgt cacccgttct gttggcttat
     4441 aatgcagggt ggggccacct gccggtaggt gtgtggtagg cttttctccg tcgcaggacg
     4501 cagggttcgg gcctagggta ggctctcctg aatcgacagg cgccggacct ctggtgaggg
     4561 gagggataag tgaggcgtca gtttctttgg tcggttttat gtacctatct tcttaagtag
     4621 ctgaagctcc ggttttgaac tatgcgctcg gggttggcga gtgtgttttg tgaagttttt
     4681 taggcacctt ttgaaatgta atcatttggg tcaatatgta attttcagtg ttagactagt
     4741 aaattgtccg ctaaattctg gccgtttttg gcttttttgt tagacggatc cataacttcg
     4801 tataatgtat gctatacgaa gttatgccac catgtctaga ctggacaaga gcaaagtcat
     4861 aaacggagct ctggaattac tcaatggtgt cggtatcgaa ggcctgacga caaggaaact
     4921 cgctcaaaag ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc
     4981 cctgctcgat gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct
     5041 ggaaggcgag tcatggcaag actttctgcg gaacaacgcc aagtcatacc gctgtgctct
     5101 cctctcacat cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta
     5161 cgaaaccctg gaaaatcagc tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc
     5221 actgtacgct ctgtccgccg tgggccactt tacactgggc tgcgtattgg aggaacagga
     5281 gcatcaagta gcaaaagagg aaagagagac acctaccacc gattctatgc ccccacttct
     5341 gagacaagca attgagctgt tcgaccggca gggagccgaa cctgccttcc ttttcggcct
     5401 ggaactaatc atatgtggcc tggagaaaca gctaaagtgc gaaagcggcg ggccgaccga
     5461 cgcccttgac gattttgact tagacatgct cccagccgat gcccttgacg actttgacct
     5521 tgatatgctg cctgctgacg ctcttgacga ttttgacctt gacatgctcc ccgggtaaat
     5581 aacttcgtat agcatacatt atacgaagta tggcgcgcca attccgcccc tctccctccc
     5641 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5701 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5761 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5821 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5881 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     5941 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     6001 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     6061 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6121 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6181 tttgaaaaac acgataatac catggccacc gagtacaagc ccacggtgcg cctcgccacc
     6241 cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     6301 acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     6361 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     6421 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     6481 tcgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     6541 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     6601 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     6661 cgcgctgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     6721 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     6781 atgacccgca agcccggtgc ctgattaatt aagcgcgtct ggaacaatca acctctggat
     6841 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6901 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6961 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     7021 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     7081 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7141 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7201 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     7261 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7321 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7381 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     7441 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     7501 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     7561 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     7621 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     7681 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     7741 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     7801 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     7861 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     7921 agaggccttg acattgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     7981 ccatctgttg tttgcccctt ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     8041 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     8101 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     8161 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     8221 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8281 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8341 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8401 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8461 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8521 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8581 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8641 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     8701 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     8761 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     8821 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     8881 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     8941 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     9001 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg atgaaaaagc
     9061 ctgaactcac cgcgacgtga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     9121 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     9181 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     9241 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     9301 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     9361 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     9421 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     9481 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     9541 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     9601 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     9661 gacgccccag cactcgtccg agggcaaagg aatagcacgt gctacgagat ttcgattcca
     9721 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     9781 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     9841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9901 cactgcattc tagttgtggt ttgtccaaac taatcaatgt atcttatcat gtctgtatac
     9961 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10021 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10081 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10141 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10201 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10261 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10321 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10381 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10441 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10501 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10561 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10621 tgtaggtcgt tcgctccaag ctgggctgtg tgcactaacc ccccgttcag cccgaccgct
    10681 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10741 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    10801 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    10861 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    10921 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10981 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11041 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11101 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11161 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11221 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11281 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11341 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11401 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11461 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11521 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11581 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11641 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11701 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11761 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11821 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11881 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11941 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12001 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12061 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12121 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
    12181 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    12241 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    12301 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    12361 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
    12421 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
    12481 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
    12541 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
    12601 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
    12661 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
    12721 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
    12781 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
    12841 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
    12901 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
    12961 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    13021 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    13081 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
    13141 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
    13201 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
    13261 tgggtgcgag agcgtcagta ttaag
//
