LOCUS       sequence-333871-       13286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses RFP cDNA and UBC9 shRNA 4 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Yu B, Swatkoski S, Holly A, Lee LC, Giroux V, Lee CS, Hsu D, Smith 
            JL, Yuen G, Yue J, Ann DK, Simpson RM, Creighton CJ, Figg WD, Gucek 
            M, Luo J
  TITLE     Oncogenesis driven by the Ras/Raf pathway requires the SUMO E2 
            ligase Ubc9.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):E1724-33. doi: 
            10.1073/pnas.1415569112. Epub 2015 Mar 24.
  PUBMED    25805818
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1415569112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "E1724-33"
COMMENT     Sequence Label: pInducer10-UBC9-sh4
FEATURES             Location/Qualifiers
     source          1..13286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    462..695
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             880..924
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1222..1339
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1404..1778)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      1775..1784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1788..1806)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1798..1845)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    join(1915..1933,1934..1956,1957..1975,1976..1998,1999..2017,
                     2018..2040,2041..2059,2060..2082,2083..2101,2102..2124,
                     2125..2143,2144..2166,2167..2185)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2218..2256
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2253..2277
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2353..3048
                     /codon_start=1
                     /product="red fluorescent protein from Entacmaea 
                     quadricolor"
                     /label=TurboRFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
                     VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
                     TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
                     LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
                     DLPSKLGHR"
     ncRNA           3099..3220
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3248..3262
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           3290..3416
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(3511..3529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        3549..4760
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4597..4616
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    4767..4800
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             join(4807..5424,5425..5430,5431..5466,5467..5469,5470..5505,
                     5506..5508,5509..5544,5545..5553)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5602..6174
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5769..5786)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5996..6015
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6177..6779
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6676..6696
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6801..7389
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6854..6874)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7272..7283)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7596..7829
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7930..7947)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7936..8160
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8206..8634
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8293..8312)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8503..8524
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8643..8663)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8648..8977
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8828..8963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8890..8909
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9800..9921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9970..9986)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9970..9986)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9983..10005)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9994..10010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10018..10024,10025..10042,10043..10048))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10063..10084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10201..10218)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10372..10960)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10452..10471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11131..11922,11923..11991))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11754..11773
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11992..12096)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12144..12278
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             12446..13079
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    13126..13251
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
       61 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      121 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
      181 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
      241 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
      301 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
      361 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
      421 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
      481 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
      541 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
      601 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
      661 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
      721 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
      781 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
      841 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
      901 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
      961 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1021 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1081 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1141 agagacagat ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca
     1201 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
     1261 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
     1321 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggttagt
     1381 accgggcccg ctctagtccg gaatcagtcc tgctcctcgg ccacgaagtg cacgcagttg
     1441 ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat ctcggtcatg
     1501 gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc ggcgtacagc
     1561 tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac ctggtcctgg
     1621 accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc gtcctccacg
     1681 aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc gacgtcgcgc
     1741 gcggtgagca ccggaacggc actggtcaac ttggccatgg tggccctcct atagtgagtc
     1801 gtattatact atgccgatat actatgccga tgattaattg tcaacacgtg ctgcaggtcc
     1861 gaggttctag gctagcttaa aggaaccaat tcagtcgact ggatccttta ccactcccta
     1921 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1981 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2041 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2101 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2161 taccactccc tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc gggtcgaggt
     2221 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2281 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagccta
     2341 ccggtcgcca ccatgagcga gctgatcaag gagaacatgc acatgaagct gtacatggag
     2401 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     2461 ggcacccaga ccatgaagat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     2521 atcctggcta ccagcttcat gtacggcagc aaagccttca tcaaccacac ccagggcatc
     2581 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagaat caccacatac
     2641 gaagacgggg gcgtgctgac cgctacccag gacaccagct tccagaacgg ctgcatcatc
     2701 tacaacgtca agatcaacgg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
     2761 acacgcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctgagaggc
     2821 cacagccaga tggccctgaa gctcgtgggc gggggctacc tgcactgctc cttcaagacc
     2881 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcttcca cttcgtggac
     2941 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgagatg
     3001 gctgtggcca agtactgcga cctccctagc aaactggggc acagatgatg cggccgcaag
     3061 ccttgttaag tgctcgcttc ggcagcacat atactatgtt gaatgaggct tcagtacttt
     3121 acagaatcgt tgcctgcaca tcttggaaac acttgctggg attacttctt caggttaacc
     3181 caacagaagg ctcgagaagg tatattgctg ttgacagtga gcgcggaata caggaacttc
     3241 taaattagtg aagccacaga tgtaatttag aagttcctgt attccttgcc tactgcctcg
     3301 gaattcaagg ggctacttta ggagcaatta tcttgtttac taaaactgaa taccttgcta
     3361 tctctttgat acatttttac aaagctgaat taaaatggta taaattaaat cacttttttc
     3421 aattggaaga ctaatgcggc cggccattag tttgaatcat ttcggtactt tgtgacgtgg
     3481 cctttctgtg gtcgcgttgt atcgttgggc cctatagtga gtcgtattac ctaggacgcg
     3541 taagatctgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
     3601 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
     3661 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
     3721 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
     3781 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
     3841 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
     3901 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
     3961 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
     4021 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
     4081 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
     4141 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
     4201 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     4261 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     4321 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     4381 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     4441 taggtgtgtg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     4501 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     4561 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     4621 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     4681 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     4741 ttttggcttt tttgttagac ggatccataa cttcgtataa tgtatgctat acgaagttat
     4801 gccaccatgt ctagactgga caagagcaaa gtcataaacg gagctctgga attactcaat
     4861 ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg agttgagcag
     4921 cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct gccaatcgag
     4981 atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg gcaagacttt
     5041 ctgcggaaca acgccaagtc ataccgctgt gctctcctct cacatcgcga cggggctaaa
     5101 gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg
     5161 ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc cgccgtgggc
     5221 cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa agaggaaaga
     5281 gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga gctgttcgac
     5341 cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg tggcctggag
     5401 aaacagctaa agtgcgaaag cggcgggccg accgacgccc ttgacgattt tgacttagac
     5461 atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc tgacgctctt
     5521 gacgattttg accttgacat gctccccggg taaataactt cgtatagcat acattatacg
     5581 aagtatggcg cgccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     5641 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     5701 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     5761 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     5821 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     5881 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     5941 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     6001 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     6061 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     6121 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
     6181 ccaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     6241 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     6301 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     6361 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     6421 gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt
     6481 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     6541 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     6601 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg
     6661 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     6721 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat
     6781 taattaagcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     6841 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6901 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6961 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     7021 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     7081 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     7141 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     7201 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     7261 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     7321 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     7381 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     7441 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     7501 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7561 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     7621 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     7681 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7741 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7801 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     7861 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc
     7921 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc cccttccccg
     7981 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     8041 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     8101 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     8161 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     8221 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8281 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8341 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8401 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8461 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8521 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8581 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     8641 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8701 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8761 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8821 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8881 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     8941 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     9001 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtgaccgca
     9061 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     9121 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     9181 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     9241 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     9301 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     9361 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     9421 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     9481 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     9541 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     9601 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     9661 aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     9721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     9781 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     9841 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     9901 caaactaatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     9961 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    10021 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10081 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10141 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10201 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10261 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10321 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10381 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10441 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10501 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10561 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10621 ctgtgtgcac taaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10681 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10741 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10801 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10861 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10921 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10981 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    11041 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    11101 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    11161 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11221 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11281 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11341 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11401 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11461 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11521 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11581 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11641 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11701 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11761 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11821 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11881 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11941 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    12001 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    12061 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    12121 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    12181 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    12241 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    12301 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    12361 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    12421 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    12481 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    12541 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    12601 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    12661 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    12721 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    12781 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    12841 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    12901 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    12961 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    13021 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    13081 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    13141 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
    13201 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
    13261 cgggggagaa ttagatcgcg atggga
//
