LOCUS       sequence-33388-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 S204A
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        52..70
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     52..69
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgctcgttt agggaaccgc cattctgcct ggggacgtcg gagcaagctt gatttaggtg
       61 acactataga atacaagcta cttgttcttt ttgcaggatc ccatcgattc gaattcacca
      121 tggactacaa ggacgacgat gacaagtcgt ccatcctgcc tttcactccc ccgatcgtga
      181 agcgcctgct gggctggaag aagggcgagc agaacgggca ggaggagaaa tggtgcgaga
      241 aggcggtcaa gagcctggtc aagaaactca agaagacggg gcagctggac gagctggaga
      301 aggccatcac cacgcagaac gtcaacacca agtgcatcac catccccagg tccctggatg
      361 gccggttgca ggtgtcccat cggaaggggc tccctcatgt catctactgc cgcctgtggc
      421 gatggccaga cctgcacagc caccacgagc tgcgggccat ggagctgtgt gagttcgcct
      481 tcaatatgaa gaaggacgag gtctgcgtga atccctacca ctaccagaga gtagagacac
      541 cagttctacc tcctgtgttg gtgccacgcc acacagagat cccggccgag ttccccccac
      601 tggacgacta cagccattcc atccccgaaa acactaactt ccccgcaggc atcgagcccc
      661 agagcaatat tccagagacc ccaccccctg gctacctgag tgaagatgga gaaaccagtg
      721 accaccagat gaaccacagc atggacgcag gtgctccaaa cctatccccg aatccgatgt
      781 ccccagcaca taataacttg gacctgcagc cagttaccta ctgcgagccg gccttctggt
      841 gctccatctc ctactacgag ctgaaccagc gcgtcgggga gacattccac gcctcgcagc
      901 catccatgac tgtggatggc ttcaccgacc cctccaattc ggagcgct
//
