LOCUS       sequence-333880-        7776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral plasmid for H2B-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Mohr L, Toufektchan E, von Morgen P, Chu K, Kapoor A, Maciejowski J
  TITLE     ER-directed TREX1 limits cGAS activation at micronuclei.
  JOURNAL   Mol Cell. 2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037.
  PUBMED    33476576
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2021 Jan 12. pii: S1097-2765(20)30958-8. doi: 
            10.1016/j.molcel.2020.12.037."
COMMENT     Sequence Label: pQCXIB-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..7776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(1..792,793..861))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     624..643
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(862..966)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1034..1052
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        1152..1531
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1532..1735
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             1736..1911
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    1974..2173
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2374..2790
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2720..2742
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2758..2774
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        2827..3130
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3131..3334
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3331..3355
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3500..3877
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     CDS             3896..4606
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4041..4059)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4309..4328
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4713..5175
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4769..4786)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4996..5015
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5192..5590
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             5705..6130
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6233..6255)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6391..6411)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6396..6725
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6576..6711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6747..6766
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6847..6864)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7018..7606)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7098..7117)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     1021 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat tagcttggcc
     1081 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
     1141 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     1201 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     1261 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     1321 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     1381 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     1441 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     1501 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     1561 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     1621 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     1681 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata
     1741 aaagagccca caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt
     1801 acccgtattc ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct
     1861 tgggagggtc tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc
     1921 gtccgggatt tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg
     1981 ccagcaactt atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg
     2041 cgtctgtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag
     2101 ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt
     2161 ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg
     2221 gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg
     2281 aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt
     2341 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
     2401 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
     2461 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     2521 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     2581 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     2641 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
     2701 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
     2761 tatccagccc tcactccttc tctaggcgcc ggaattgaag atctggggga tcgatcctct
     2821 agagtccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2881 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2941 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     3001 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     3061 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     3121 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     3181 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     3241 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     3301 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     3361 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     3421 cggccgggaa cggtgcattg gaacggacct gcaggaattg atccgcggcc gcaccggtag
     3481 gcctcgtacg cttaattaaa tgccagagcc agcgaagtct gctcccgccc cgaaaaaggg
     3541 ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc aagaagcgca agcgcagccg
     3601 caaggagagc tattccatct atgtgtacaa ggttctgaag caggtccacc ctgacaccgg
     3661 catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg aacgacattt tcgagcgcat
     3721 cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc tcgaccatca cctccaggga
     3781 gatccagacg gccgtgcgcc tgctgctgcc tggggagttg gccaagcacg ccgtgtccga
     3841 gggtactaag gccatcacca agtacaccag cgctaaggat ccaccgggcg gccgcatggt
     3901 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3961 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     4021 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     4081 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     4141 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     4201 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     4261 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     4321 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     4381 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4441 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4501 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4561 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtgaggat ccggaatccc
     4621 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     4681 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     4741 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     4801 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     4861 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     4921 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     4981 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     5041 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     5101 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     5161 aaaacacgat gataagcttg atcacgcgtc catggccaag cctttgtctc aagaagaatc
     5221 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     5281 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     5341 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     5401 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     5461 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     5521 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     5581 ggagggctaa gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     5641 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     5701 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     5761 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     5821 gaacagggtc gaccctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat
     5881 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt
     5941 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga
     6001 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact
     6061 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg
     6121 gtctttcatt tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca
     6181 ccaccgggag gtaagctggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6241 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6301 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     6361 gtagcgatag cggagtgtag atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt
     6421 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6481 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6541 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     6601 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     6661 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     6721 gcaaaaagct tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     6781 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     6841 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6901 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6961 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7021 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7081 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7141 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7201 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7261 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7321 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7381 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7441 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7501 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7561 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7621 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7681 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7741 aatctaaagt atatatgagt aaacttggtc tgacag
//
