LOCUS       sequence-333881-       13397 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  puromycin resistant lentiviral vector for expression of PAK5 M173I.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13397)
  AUTHORS   LaPak KM, Vroom DC, Garg AA, Guan X, Hays JL, Song JW, Burd CE
  TITLE     Melanoma-associated mutants within the serine-rich domain of PAK5 
            direct kinase activity to mitogenic pathways.
  JOURNAL   Oncotarget. 2018 May 22;9(39):25386-25401. doi: 
            10.18632/oncotarget.25356. eCollection 2018 May 22.
  PUBMED    29875996
REFERENCE   2  (bases 1 to 13397)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.18632/oncotarget.25356"; journalName: "Oncotarget"; date: 
            "2018-05-22- 22"; volume: "9"; issue: "39"; pages: "25386-25401"
COMMENT     Sequence Label: pLenti-3xFLAG-Puro PAK5 M173I
FEATURES             Location/Qualifiers
     source          1..13397
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     primer_bind     3704..3723
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             3883..3948
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    6174..6747
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6342..6359)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6569..6588
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6750..7352
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7249..7269
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7368..7956
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7421..7441)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7839..7850)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8163..8396
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8497..8514)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8503..8727
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8773..9201
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8860..8879)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9070..9091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9210..9230)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9215..9544
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9395..9530
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9457..9476
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10752..10873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10922..10938)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10922..10938)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10935..10957)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10946..10962
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10970..10976,10977..10994,10995..11000))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11015..11036
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11153..11170)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11324..11912)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11404..11423)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12083..12874,12875..12943))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12706..12725
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12944..13048)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    13096..13230
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta ggctgccacg tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc
     2761 ggacgctcag gacagcggcc cgctgctcat aagactcggc cttagaaccc cagtatcagc
     2821 agaaggacat tttaggacgg gacttgggtg actctagggc actggttttc tttccagaga
     2881 gcggaacagg cgaggaaaag tagtcccttc tcggcgattc tgcggaggga tctccgtggg
     2941 gcggtgaacg ccgatgatta tataaggacg cgccgggtgt ggcacagcta gttccgtcgc
     3001 agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta
     3061 gcgggctgct gggctggccg gggctttcgt ggccgccggg ccgctcggtg ggacggaagc
     3121 gtgtggagag accgccaagg gctgtagtct gggtccgcga gcaaggttgc cctgaactgg
     3181 gggttggggg gagcgcagca aaatggcggc tgttcccgag tcttgaatgg aagacgcttg
     3241 tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca tggtgggcgg caagaaccca
     3301 aggtcttgag gccttcgcta atgcgggaaa gctcttattc gggtgagatg ggctggggca
     3361 ccatctgggg accctgacgt gaagtttgtc actgactgga gaactcggtt tgtcgtctgt
     3421 tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
     3481 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
     3541 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
     3601 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     3661 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     3721 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     3781 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     3841 tggccgtttt tggctttttt gttagacgct agcggatcca tggactacaa agaccatgac
     3901 ggtgattata aagatcatga tatcgattac aaggatgacg atgacaagct agcgatgttt
     3961 gggaagaaaa agaaaaagat tgaaatatct ggcccgtcca actttgaaca cagggttcat
     4021 actgggtttg atccacaaga gcagaagttt accggccttc cccagcagtg gcacagcctg
     4081 ttagcagata cggccaacag gccaaagcct atggtggacc cttcatgcat cacacccatc
     4141 cagctggctc ctatgaagac aatcgttaga ggaaacaaac cctgcaagga aacctccatc
     4201 aacggcctgc tagaggattt tgacaacatc tcggtgactc gctccaactc cctaaggaaa
     4261 gaaagcccac ccaccccaga tcagggagcc tccagccacg gtccaggcca cgcggaagaa
     4321 aatggcttca tcaccttctc ccagtattcc agcgaatccg atactactgc tgactacacg
     4381 accgaaaagt acagggagaa gagtctctat ggagatgatc tggatccgta ttatagaggc
     4441 agccacgcag ccaagcaaaa tgggcacgta atcaaaatga agcacgggga ggcctactat
     4501 tctgaggtga agcctttgaa atccgatttt gccagatttt ctgccgatta tcactcacat
     4561 ttggactcac tgagcaaacc aagtgaatac agtgacctca agtgggagta tcagagagcc
     4621 tcgagtagct cccctctgga ttattcattc caattcacac cttctagaac tgcagggacc
     4681 agcgggtgct ccaaggagag cctggcgtac agtgaaagtg aatggggacc cagcctggat
     4741 gactatgaca ggaggccaaa gtcttcgtac ctgaatcaga caagccctca gcccaccatg
     4801 cggcagaggt ccaggtcagg ctcgggactc caggaaccga tgatgccatt tggagcaagt
     4861 gcatttaaaa cccatcccca aggacactcc tacaactcct acacctaccc tcgcttgtcc
     4921 gagcccacaa tgtgcattcc aaaggtggat tacgatcgag cacagatggt cctcagccct
     4981 ccactgtcag ggtctgacac ctaccccagg ggccctgcca aactacctca aagtcaaagc
     5041 aaatcgggct attcctcaag cagtcaccag tacccgtctg ggtaccacaa agccaccttg
     5101 taccatcacc cctccctgca gagcagttcg cagtacatct ccacggcttc ctacctgagc
     5161 tccctcagcc tctcatccag cacctacccg ccgcccagct ggggctcctc ctccgaccag
     5221 cagccctcca gggtgtccca tgaacagttt cgggcggccc tgcagctggt ggtcagccca
     5281 ggagacccca gggaatactt ggccaacttt atcaaaatcg gggaaggctc aaccggcatc
     5341 gtatgcatcg ccaccgagaa acacacaggg aaacaagttg cagtgaagaa aatggacctc
     5401 cggaagcaac agagacgaga actgcttttc aatgaggtcg tgatcatgcg ggattaccac
     5461 catgacaatg tggttgacat gtacagcagc taccttgtcg gcgatgagct ctgggtggtc
     5521 atggagtttc tagaaggtgg tgccttgaca gacattgtga ctcacaccag aatgaatgaa
     5581 gaacagatag ctactgtctg cctgtcagtt ctgagagctc tctcctacct tcataaccaa
     5641 ggagtgattc acagggacat aaaaagtgac tccatcctcc tgacaagcga tggccggata
     5701 aagttgtctg attttggttt ctgtgctcaa gtttccaaag aggtgccgaa gaggaaatca
     5761 ttggttggca ctccctactg gatggcccct gaggtgattt ctaggctacc ttatgggaca
     5821 gaggtggaca tctggtccct cgggatcatg gtgatagaaa tgattgatgg cgagcccccc
     5881 tacttcaatg agcctcccct ccaggcgatg cggaggatcc gggacagttt acctccaaga
     5941 gtgaaggacc tacacaaggt ttcttcagtg ctccggggat tcctagactt gatgttggtg
     6001 agggagccct ctcagagagc aacagcccag gaactcctcg gacatccatt cttaaaacta
     6061 gcaggtccac cgtcttgcat tgtccccctc atgagacaat acaggcatca ctgaattctg
     6121 cagatatcca gcacagtggc ggccgctcga gtctagagtt aacgcaaatt ccgcccctct
     6181 ccctcccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     6241 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     6301 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     6361 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     6421 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     6481 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     6541 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     6601 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg
     6661 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     6721 ggttttcctt tgaaaaacac gataatacca tggccaccga gtacaagccc acggtgcgcc
     6781 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
     6841 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
     6901 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     6961 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     7021 agatcggctc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     7081 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     7141 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     7201 cggccgagcg cgctggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     7261 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     7321 cctggtgcat gacccgcaag cccggtgcct gagttcgcgt ctggaacaat caacctctgg
     7381 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     7441 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     7501 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     7561 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     7621 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     7681 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     7741 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     7801 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     7861 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     7921 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct gcagtcgaga
     7981 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     8041 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     8101 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagagggg
     8161 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     8221 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8281 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8341 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     8401 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     8461 tgagaggcct tgacattgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     8521 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     8581 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     8641 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     8701 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     8761 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     8821 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     8881 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     8941 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     9001 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     9061 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     9121 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     9181 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     9241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     9301 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     9361 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     9421 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     9481 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     9541 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgatgaaaaa
     9601 gcctgaactc accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc
     9661 cgacctgatg cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg
     9721 gcgtggatat gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt
     9781 ttatcggcac tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt
     9841 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     9901 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     9961 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
    10021 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
    10081 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
    10141 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
    10201 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
    10261 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
    10321 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
    10381 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
    10441 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
    10501 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
    10561 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
    10621 agcacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
    10681 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
    10741 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
    10801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
    10861 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
    10921 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10981 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    11041 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    11101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    11161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    11221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    11281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    11341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    11401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    11461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    11581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    12001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    12061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    12121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    12181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    12241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    12301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    12361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    12421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    12481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    12541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    12601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    12661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    12721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    12781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    12841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    12901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    12961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    13021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    13081 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    13141 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13201 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    13261 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    13321 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    13381 actacaaact tagtagt
//
