LOCUS       sequence-333884-        5007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses owl monkey retroCHMP3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Rheinemann L, Downhour DM, Davenport KA, McKeown AN, Sundquist WI, 
            Elde NC
  TITLE     Recurrent evolution of an inhibitor of ESCRT-dependent virus budding
            and LINE-1 retrotransposition in primates.
  JOURNAL   Curr Biol. 2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018.
  PUBMED    35245459
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol. 
            2022 Feb 25. pii: S0960-9822(22)00240-8. doi: 
            10.1016/j.cub.2022.02.018."
COMMENT     Sequence Label: pEF1-alpha-owl-monkey-retroCHMP3-HA
FEATURES             Location/Qualifiers
     source          1..5007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(208..224)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(208..224)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(221..243)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    232..248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(256..262,263..280,281..286))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    301..322
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        381..576
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      427..562
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    582..716
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(619..638)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     673..692
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(784..801)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(955..1543)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1035..1054)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1714..2505,2506..2574))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2337..2356
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2575..2679)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2741..3923
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2972..3914
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3871..3891
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4619..4645
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(4734..4753)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4799..4854
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4853..4872)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
       61 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
      121 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
      181 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
      241 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
      301 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      361 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
      421 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
      481 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
      541 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
      601 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      661 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
      721 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      781 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      841 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      901 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      961 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1021 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1081 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1141 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1201 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1261 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1381 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2701 acctgggtcg acattgatta ttgactagat catcgcgtga ggctccggtg cccgtcagtg
     2761 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2821 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2881 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2941 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     3001 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacgc ccctggctgc
     3061 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     3121 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     3181 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     3241 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     3301 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     3361 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     3421 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     3481 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     3541 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3601 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3661 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3721 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3781 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3841 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3901 ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgac ggtacccaat ttgccatggg
     3961 gcttttggtt aaaacccagg agaagccacc acaagaactg gtcaatgaat ggtcactgaa
     4021 gataagaaag gaaatgagag ctgttcacag gcaaataagg gatatccaaa gaggagaaga
     4081 aaaagtgaaa cgatctgtga aagatgcagt caagaagggc cagaaggatg tctgtgtagt
     4141 tctggccagg gagatgatca ggtcagggaa ggccatgagc aagctgtatg catccaaagc
     4201 gcccatgaac ttggtgctca tggggatgag gaaccagctc gtggtcttgc gagtggctgg
     4261 ttccttgcag aagagcacag aagtgatgag ggctgtgcaa agtcttgtga agattccaga
     4321 aattcaggcc accatgaggg agctgtccaa agaaatgatg aaggctggga tcatagagga
     4381 gacgttagag gacactttcg aaagcatgga tgatcaggaa gaaatggagg aagaagcaga
     4441 aatggaaatt gacaaaattc tatttgaaat tacagcaggg gccttgggca aagcacccag
     4501 taaagtgact gatgcccttc cagagccaga accttcagga gcgatggccg cctcagagga
     4561 cgaggaggag gaagaagctc tggaggccat gcagtcccag ctggccacac tccacagcta
     4621 cccatacgat gttccagatt acgcttgagc ggccgcactc ctcaggtgca ggctgcctat
     4681 cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt
     4741 ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa
     4801 taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga
     4861 aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg
     4921 gcaacatatg cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag
     4981 tatatgaaac agccccctgc tgtccat
//
