LOCUS       sequence-33389-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Gao S, Alarcon C, Sapkota G, Rahman S, Chen PY, Goerner N, Macias 
            MJ, Erdjument-Bromage H, Tempst P, Massague J
  TITLE     Ubiquitin ligase Nedd4L targets activated Smad2/3 to limit TGF-beta 
            signaling.
  JOURNAL   Mol Cell. 2009 Nov 13. 36(3):457-68.
  PUBMED    19917253
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2009 Nov 13. 36(3):457-68."
COMMENT     Sequence Label: pCS2 Flag Smad3 S204A
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..40
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        22..40
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    157..291
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(194..213)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     248..267
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tgggtaccgg
       61 gcccaatgca ttggcgccgc ggcgcgccgc gaattaaaaa acctcccaca cctccccctg
      121 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
      181 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
      241 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata
      301 cgactcacta tagttctaga ggctcgagag gccttgcggc cgcggccaga ttggcctgtc
      361 gacgaattcc tgcagcccga tgtctctaag acacactgga acagcggatg cttggggagc
      421 ccatctgggt gaggaccttg tcaagccact gcaaaggccc attcaggtgc agctcaatcc
      481 agcagggggt actggtcaca gtctgtctcc tgtactccgc tccccagcct ttgacgaagc
      541 tcatgcggat ggtgcacatt cgggtcaact ggtagacagc ctcaaagccc tggttgaccg
      601 actgggccag gagggcagcg aactcctggt tgttgaagat cttcaggttg catcctggtg
      661 ggatcttgca gacggtggcc gggtgccagc catagcgctg gttacagttg ggagactgga
      721 caaaaatagc gctgtcactg aggcactctg cgaagacctc ccctccgatg tagtagagcc
      781 gcacgcctct tccgatgtgt ctccgtgtca gctccactgc tgcattcct
//
