LOCUS       sequence-333925-        2022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin 
            resistance, optimal growth at 20 degrees..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Arslan S, Khafizov R, Thomas CD, Chemla YR, Ha T
  TITLE     Protein structure. Engineering of a superhelicase through 
            conformational control.
  JOURNAL   Science. 2015 Apr 17;348(6232):344-7. doi: 10.1126/science.aaa0445.
  PUBMED    25883358
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2015-04-17- 17"; volume: "348"; 
            issue: "6232"; pages: "344-7"
COMMENT     Sequence Label: RepX_pET28a(+)
FEATURES             Location/Qualifiers
     source          1..2022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgcgtctaa accccggcca acaacaagct gtcgaattcg ttaccggccc cttgctggtg
       61 ctggcgggcg cgggttccgg taaaactcgt gttatcacca ataaaatcgc ccatctgatc
      121 cgcggtagcg ggtaccaggc gcggcacatt gcggcggtga cctttactaa taaagcagcg
      181 cgcgagatga aagagcgtgt agggcagacg ctggggcgca aagaggcgcg tgggctgatg
      241 atctccactt tccatacgtt ggggctggat atcatcaaac gcgagtatgc ggcgcttggg
      301 atgaaagcga acttctcgtt gtttgacgat accgatcagc ttgctttgct taaagagttg
      361 accgaggggc tgattgaaga tgacaaagtt ctcctgcaac aactgatttc gaccatctct
      421 aactggaaga atgatctcaa aacaccgtcc caggcggcag caagtgcgat tggcgagcgg
      481 gaccgtattt ttgcccatgt ttatgggctg tatgatgcac acctgaaagc ctgtaacgtt
      541 ctcgacttcg atgatctgat tttattgccg acgttgctgc tgcaacgcaa tgaagaagtc
      601 cgcaagcgct ggcagaacaa aattcgctat ctgctggtgg atgagtatca ggacaccaac
      661 accagccagt atgagctggt gaaactgctg gtgggcagcc gcgcgcgctt taccgtggtg
      721 ggtgacgatg accagtcgat ctactcctgg cgcggtgcac gtccgcaaaa cctggtgctg
      781 ctgagtcagg attttccggc gctgaaggtg attaagcttg agcagaacta tcgctcttcc
      841 gggcgtattc tgaaagcggc gaacatcctg atcgccaata acccgcacgt ctttgaaaag
      901 cgtctgttct ccgaactggg ttatggcgcg gagctaaaag tattaagcgc gaataacgaa
      961 gaacatgagg ctgagcgcgt tactggcgag ctgatcgccc atcacttcgt caataaaacg
     1021 cagtacaaag attacgccat tctttatcgc ggtaaccatc agtcgcgggt gtttgaaaaa
     1081 ttcctgatgc aaaaccgcat cccgtacaaa atatctggtg gtacgtcgtt tttctctcgt
     1141 cctgaaatca aggacttgct ggcttatctg cgcgtgctga ctaacccgga cgatgactgc
     1201 gcatttctgc gtatcgttaa cacgccgaag cgagagattg gcccggctac gctgaaaaag
     1261 ctgggtgagt gggcgatgac gcgcaataaa agcatgttta ccgccagctt tgatatgggc
     1321 ctgagtcaga cgcttagcgg acgtggttat gaagcattga cccgcttcac tcactggttg
     1381 gcagaaatcc agcgtctggc ggagcgggag ccgattgccg cggtgcgtga tctgatccat
     1441 ggcatggatt atgaatcctg gctgtacgaa acatcgccca gcccgaaagc cgccgaaatg
     1501 cgcatgaaga acgtcaacca actgtttagc tggatgacgg agatgctgga aggcagtgaa
     1561 ctggatgagc cgatgacgct cacccaggtg gtgacgcgct ttactttgcg cgacatgatg
     1621 gagcgtggtg agagtgaaga agagctggat caggtgcaac tgatgactct ccacgcgtcg
     1681 aaagggctgg agtttcctta tgtctacatg gtcggtatgg aagaagggtt tttgccgcac
     1741 cagagcagca tcgatgaaga taatatcgat gaggagcggc ggctggccta tgtcggcatt
     1801 acccgcgccc agaaggaatt gacctttacg ctggctaaag aacgccgtca gtacggcgaa
     1861 ctggtgcgcc cggagccgag ccgctttttg ctggagctgc cgcaggatga tctgatttgg
     1921 gaacaggagc gcaaagtggt cagcgccgaa gaacggatgc agaaagggca aagccatctg
     1981 gcgaatctga aagcgatgat ggcggcaaaa cgagggaaat aa
//
