LOCUS       sequence-333930-       11096 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. 
            pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11096)
  TITLE     Carr lab yeast expression plasmids
REFERENCE   2  (bases 1 to 11096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAW8-aid-spo5DSR-Turg1:kanMX6
FEATURES             Location/Qualifiers
     source          1..11096
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      52..640
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     541..560
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     794..811
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    928..949
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(964..969,970..987,988..994)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1002..1018
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1007..1029
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1026..1042
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1026..1042
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(1074..1107)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1146..1835
                     /codon_start=1
                     /gene="Arabidopsis thaliana AXR3"
                     /product="auxin-responsive IAA17 protein"
                     /label=auxin-inducible degron
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="MMGSVELNLRETELCLGLPGGDTVAPVTGNKRGFSETVDLKLNLN
                     NEPANKEGSTTHDVVTFDSKEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSS
                     GGPEAAAFVKVSMDGAPYLRKIDLRMYKSYDELSNALSNMFSSFTMGKHGGEEGMIDFM
                     NERKLMDLVNSWDYVPSYEDKDGDWMLVGDVPWPMFVDTCKRLRLMKGSDAIGLAPRAM
                     EKCKSRA"
     gene            2583..3939
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        2583..2926
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             2927..3736
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2994..3013)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      3742..3939
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(4034..4051)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4034..4050)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4043..4065)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6945..8039
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     rep_origin      8871..9652
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     primer_bind     complement(9747..9766)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9866..9888
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9926..9944)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10012..10116
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10117..10185,10186..10977)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10335..10354)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
       61 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      121 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      181 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      241 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      301 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      361 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      421 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      481 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      541 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      601 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      661 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      721 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      781 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
      841 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
      901 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
      961 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1021 tcacacagga aacagctatg accatgatta cgccaagctt gcatggcggc cgcataactt
     1081 cgtatagcat acattatacg aagttatgca tgctcggtgg gtcaggtgga agtggatctg
     1141 gtgctatgat gggcagtgtc gagctgaatc tgagggagac tgagctgtgt cttggtcttc
     1201 ccggtggaga tacagtggct ccggtaaccg gaaacaagag agggttctca gagacggttg
     1261 atctgaagct aaatctgaat aatgagcctg caaacaagga aggatctacg actcatgacg
     1321 tcgtgacttt tgattccaag gagaagagtg cttgtcctaa agatccagcc aaacctccgg
     1381 ccaaggcaca agttgtggga tggccaccgg tgagatcata ccggaagaac gtgatggttt
     1441 cctgccaaaa atcaagcggt ggcccggagg cggcggcgtt cgtgaaggta tcaatggacg
     1501 gagcaccgta cttgaggaaa atcgatttga ggatgtataa aagctacgat gagctttcta
     1561 atgctttgtc caacatgttc agctctttta ccatgggcaa acatggagga gaagaaggaa
     1621 tgatagactt catgaatgag aggaaattga tggatttggt gaatagctgg gactatgttc
     1681 cctcttatga agacaaagac ggtgattgga tgctcgtcgg cgacgttcct tggccaatgt
     1741 tcgtcgatac atgcaagcgt ttacgtctca tgaaaggatc ggatgccatt ggtctcgctc
     1801 cgagggcgat ggagaagtgc aagagcagag cttgagctag cactacgcca tatcatgccc
     1861 atcccgctac ttctgctgct aattaaattt aaacatcttt ccagcattta cttgaacatc
     1921 aaactctaat atcgaaatct tttcaaacac ttatcacata ctcaaactta aaccaacata
     1981 aaacctgtta gacaaagccc cggggcttgc ccatctgttt tagacgtcac agaaggacca
     2041 tgtttatttt aaatttcaca tacatgtgtt taattacctt cattgtataa attctcgaaa
     2101 ctttctccaa ttcttcggtc agataaaaaa tttctctagt caccgtcttt tatagagctt
     2161 ttgtcagatg tttttctgga tggaatcatt gtccagttac tatgcttttt atttataagt
     2221 ttgaaagttt agttaacttt ttgttttctt taatacattt tttaatcatg tcaacatttg
     2281 ggatttgtag aagtagtaaa ttttttgtac atgttgtaga attggataag aacgaaaaca
     2341 gctagtatgt aaaaaaaacg aaaatcccaa attccgttgt ttgtttgata tagtaatatt
     2401 tatagcattt tagtttctaa agatttttga taatctttta catttgcttt cacatacatt
     2461 cgcgctacta ccatacatca tatcccgctt tctttaattg cttccccaca gactcaacca
     2521 gcggggcctt cttctcgaga tctgtttagc ttgcctcgtc cccgccgggt cacccggcca
     2581 gcgacatgga ggcccagaat accctccttg acagtcttga cgtgcgcagc tcaggggcat
     2641 gatgtgactg tcgcccgtac atttagccca tacatcccca tgtataatca tttgcatcca
     2701 tacattttga tggccgcacg gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg
     2761 agcagggaaa cgctcccctc acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga
     2821 gaaatataaa aggttaggat ttgccactga ggttcttctt tcatatactt ccttttaaaa
     2881 tcttgctagg atacagttct cacatcacat ccgaacataa acaaccatgg gtaaggaaaa
     2941 gactcacgtt tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa
     3001 atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc
     3061 cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga
     3121 tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt
     3181 tatccgtact cctgatgatg catggttact caccactgcg atccccggca aaacagcatt
     3241 ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt
     3301 cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt
     3361 tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga
     3421 tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc
     3481 attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga
     3541 cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca
     3601 ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct
     3661 ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct
     3721 cgatgagttt ttctaatcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca
     3781 tttgtatagt ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat
     3841 attttttttc gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata
     3901 tcatgcgtca atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc
     3961 cgccatccag tcgacgaact agtataactt cgtatatggt attatatacg aagttatgcg
     4021 gccgcgcgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     4081 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     4141 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
     4201 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataccatcg tccggatcaa
     4261 tgaagtagat gagtttacta cctgtatata cttttttttt tgtcaaagtc acttttatgg
     4321 catttccatg ttgaaggatt agccacagca ctaaattttc agcgtatgat tgcttttaaa
     4381 tatttaattt tcatcgtttt ttaattaata ttcaaaacga tttaatgcct tctacatctg
     4441 aagtcaagct catagactcg ggttgggtag ctaaaactac atctaacacg aaggggtata
     4501 gccttcttaa agtatactca tgtataaacg ttttgggttc atatttttgg gccacgtggt
     4561 ttataaaaat tcttaactac accactcggt atcccgcacc cgtctacgtt tctacgattt
     4621 cgagctaata ttgagtaaag tgattagcaa aaaaaataaa agtactcgtt gtcggagatc
     4681 aagaattttt ctattatctc atctaaacca ctttctaaaa gcgaaaaaca aaatcgtaat
     4741 atgcagcttg aatgggcttc catagtttga aagaaaaacc ctagcagtac tggcaaggga
     4801 gacattcctt ttacctggct aatcgccatc ttccagcagg cgcaccattg cccctgtttc
     4861 actatccagg ttacggatat agttcatgac aatatttaca ttggtccagc caccagcttg
     4921 catgatctcc ggtattgaaa ctccagcgcg ggccatatct cgcgcggctc cgacacgggc
     4981 actgtgtcca gaccaggcca ggtatctctg accagagtca tccttagcgc cgtaaatcaa
     5041 tcgatgagtt gcttcaaaaa tcccttccag ggcgcgagtt gatagctggc tggtggcaga
     5101 tggcgcggca acaccatttt ttctgacccg gcaaaacagg tagttattcg gatcatcagc
     5161 tacaccagag acggaaatcc atcgctcgac cagtttagtt acccccaggc taagtgcctt
     5221 ctctacacct gcggtgctaa ccagcgtttt cgttctgcca atatggatta acattctccc
     5281 accgtcagta cgtgagatat ctttaaccct gatcctggca atttcggcta tacgtaacag
     5341 ggtgttataa gcaatcccca gaaatgccag attacgtata tcctggcagc gatcgctatt
     5401 ttccatgagt gaacgaacct ggtcgaaatc agtgcgttcg aacgctagag cctgttttgc
     5461 acgttcaccg gcatcaacgt tttcttttcg gatccgccgc ataaccagtg aaacagcatt
     5521 gctgtcactt ggtcgtggca gcccggaccg acgatgaagc atgtttagct ggcccaaatg
     5581 ttgctggata gtttttactg ccagaccgcg cgcctgaaga tatagaagat aatcgcgaac
     5641 atcttcaggt tctgcgggaa accatttccg gttattcaac ttgcaccatg ccgcccacga
     5701 ccggcaaacg gacagaagca ttttccaggt atgctcagaa aacgcctggc gatccctgaa
     5761 catgtccatc aggttcttgc gaacctcatc actcgttgca tcgaccggta acttttagat
     5821 taacatttgt ttaaatatga aattaggcaa tgtaacatac tgcaggcaaa ttttggtgta
     5881 cggtaagtaa attggacata tgatttaaca aagcgactat aagtcagaaa gtgagaatga
     5941 gattgaaata attaattcaa cttattcaat tgatgatatg ccaggattcc tcttccttta
     6001 tcttcaatgt ttcgattaac aggtccatta tccggttttt agtttcgctg cacattgccg
     6061 aatgacaatc ggattccttt aagctttgct tttgccctgt tttctctccc cagtaatcgt
     6121 tgggtgactg aaccatccag tagccgagag ttacgttttt cctgatttaa taaatatcca
     6181 tatctatttg tctcgtggtg ttttacagca actaaccgaa attactattt tgcatcatcc
     6241 aaccataaag agacaacaat aattctagaa aacatataac aagtgttttt ttctatttac
     6301 gtctcatacg caaagtccac tttaccatgt tttggaaact tgtacccaac cattcatttt
     6361 tcgattttgt aatgaggctc ttttcttcta acaatcattg aggtcattat catgtgtcct
     6421 cacctattgt ttaaattgag gcttgcgcta ttcccaaaat tgtgggtatt attttcatat
     6481 atcagtatct gcaatttcgg ttcaactccg gaagctcggt acccaactgt gggaatactc
     6541 aggtatcgta agatgcaaga gttcgaatct cttagcaacc attatttttt ttctctcaac
     6601 ataacgagaa cacacagggg cgctatcgca cagaatcaaa ttcgatgact ggaaattttt
     6661 tgttaatttc agaggtcgcc tgacgcatat acctttttca actgaaaaat tgggagaaaa
     6721 aggaaaggtg agagcgccgg aaccggcttt tcatatagaa tagagaagcg ttcatgacta
     6781 aatgcttgca tcacaatact tgaagttgac aatattattt aaggacctat tgttttttcc
     6841 aataggtggt tagcaatcgt cttactttct aacttttctt accttttaca tttcagcaat
     6901 atatatatat atatatatat atatttcaag gatataccat tgtaatgtct gcccctaaga
     6961 agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat cacagccgaa gccattaagg
     7021 ttcttaaagc tatttctgat gttcgttcca atgtcaagtt cgatttcgaa aatcatttaa
     7081 ttggtggtgc tgctatcgat gctacaggtg ttccacttcc agatgaggcg ctggaagcct
     7141 ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg tcctaaatgg ggtaccggta
     7201 gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga acttcaattg tacgccaact
     7261 taagaccatg taactttgca tccgactctc ttttagactt atctccaatc aagccacaat
     7321 ttgctaaagg tactgacttc gttgttgtta gagaattagt gggaggtatt tactttggta
     7381 agagaaagga agacgatggt gatggtgtcg cttgggatag tgaacaatac accgttccag
     7441 aagtgcaaag aatcacaaga atggccgctt tcatggccct acaacatgag ccaccattgc
     7501 ctatttggtc cttggataaa gctaatgttt tggcctcttc aagattatgg agaaaaactg
     7561 tggaggaaac catcaagaac gaatttccta cattgaaagt tcaacatcaa ttgattgatt
     7621 ctgccgccat gatcctagtt aagaacccaa cccacctaaa tggtattata atcaccagca
     7681 acatgtttgg tgatatcatc tccgatgaag cctccgttat cccaggctcc ttgggtttgt
     7741 tgccatctgc gtccttggcc tctttgccag acaagaacac cgcatttggt ttgtacgaac
     7801 catgccatgg ttccgctcca gatttgccaa agaataaggt caaccctatc gccactatct
     7861 tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc tgaagaaggt aaagccattg
     7921 aagatgcagt taaaaaggtt ttggatgcag gtatcagaac tggtgattta ggtggttcca
     7981 acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt taagaaaatc cttgcttaaa
     8041 aagattctct ttttttgtga tatttgtaca taaactttat aaatgaaatt cataatagaa
     8101 acgacacgaa attacaaaat ggaatatgtt catagggtag acgaaactat atacgcaatc
     8161 tacatacatt tatcaagaag gagaaaaagg aggatgtaaa ggaatacagg taagcaaatt
     8221 gatactaatg gctcaacgtg ataaggaaaa agaattgcac tttaacatta atattgacaa
     8281 ggaggagggc atcacacaaa aagttaggtg taacagaaaa tcatgaaact atgatttcta
     8341 atttatatat tggaggattt tctctaaaaa aaaaaataca acaaataaaa aacactcaat
     8401 gacctgacca tttgatggag tttaagtcaa taccttcttg aaccatttcc cataatggtg
     8461 aaagttccct caagaatttt actctgtcag aaacggcctt aacgacgtag tcgatccgga
     8521 cgatggccaa ccttccaatt cattaaatcg agtcctccat cagatgattt acaaggtttt
     8581 gcatagaatc cctaagtaag agaagttgtt agtgatttct tcaagtttac taaaatttac
     8641 atacaaatgg atgatctctt ctccattgtt tcctttcttc ttgcaaacgc gttttacaaa
     8701 gagatgacat gctattttct gattattttt ttctatagtt ttctattttg tacgtccttg
     8761 ggagtccgaa atgtaaaatc ggggtatggt tagtagcggc agcgttcatt atggtatata
     8821 aaagataaaa agtaggggca agcgagacag aatgggatac aagggcatcg tctataatta
     8881 tagctaaaaa ttgtatttta atttgtattt tttgtaattt tatttctcat tgttttactt
     8941 aaaatgaatg cgaattagag aaaacttcaa cgaaatgtca aaataagctc agcaaaatat
     9001 acaattttag ggaaagcgat cagcaactta ttctccgttg tagattcttt ttcttcataa
     9061 attccaacaa tgtaactgcc aattgatttt tctctagatg ctaataaatt gaaactaatg
     9121 aatataataa cacaggtttt gataaattgt aaactcttac tcaaaaaaag aatgttaaaa
     9181 ctaatttaac ctttttataa aggctcatgg cgttaaagag gatagtaaaa attctacaat
     9241 tttttaattt aatgtcacaa ttgtcttgcg aatataattc tattaatttt cgctaactgc
     9301 tgttttagta ctattacttt caatatgcct tatatgcaac tttaattcat gaagaatgca
     9361 atatttttgc aatctttctt gtactgagcg ctttttatca tttagtactc attatttaat
     9421 ttttcagtaa agggcaataa ataaatttgt agaagatgca atgtaatctc ctctatcctt
     9481 ttgctcatat gtttatgagt atacctagtc tagaaaggct tgtatttaaa atattattca
     9541 ataaaaattc acaattttta cgacatgtgc taccattcac ttaacttcct gattataaaa
     9601 ttggttcgtt tatactaatt acttaagtac ctttaactaa acaaaatgcc tatatatata
     9661 ttaatttaca atgagtgtca gataagtcac tatgtccgag tggttaagga gttagactcg
     9721 ccatcgatgg tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
     9781 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9841 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9901 tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc
     9961 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
    10021 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
    10081 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
    10141 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
    10201 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
    10261 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
    10321 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
    10381 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
    10441 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
    10501 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
    10561 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
    10621 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
    10681 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
    10741 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
    10801 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
    10861 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
    10921 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
    10981 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
    11041 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtga
//
